• About
• Search
• MiMIC
  • Technique
  • Constructs
  • RMCE Lines
• Transposons
  • GDP
  • Others
• CRIMIC
  • Technique
  • Constructs
• Order
• Downloads

CRIMIC CR00407

Gene Tg

CG7356

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:8024325..8024348	28D3	FBti0199581	79255

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no/short band	OK	GCAAGGAACCTCCACTGGCTCATAAATG	GACCACACTGAAGCTCTGCCCAATCTTAA

Images

Construct Design Sequences

sgRNA sequence	ATGAGATCATCGGCTGCCACAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GAAACGTGCCGAACAAGGTG	TATTCCCCAAAGCTGCAGCA	ATGAGATCATCGGCTGCCAC	GAGTCCGTGGTCTCTGCATC

 

Amplicon 5'

Amplicon 3'

GAAACGTGCCGAACAAGGTGATCAGATCAGATCAGCCATGTGGAAACAAATGCCCCAATAAATCATAATTGCAAGTAAAATCGATTTATTTTCGATGCTTTACCACTTAAAGAGCTTACTCTAAAAATGCTTTTATTTATTGCTCAAAAAAAAAATCCGTAGATGCTCATGAGGAAGAAATCGAAGAAATCTATTTTTATTTCAAGCAATGCGACCCTCAGTGACCTCAGTAAATTGAATTTCATTCATTAAATACTTTATTCTCCCTTTCAAAAAAGCAAACTGTTACCAAAAAAAAAAAACTAAAAGTCATTTTTATGAAAAGTATTGTTTTCTAATCTGATCTAATTAACCAGTGAGCCCCCATTTTCACTTTCATCCATAACTTTTTAACTTTGCAACTTTTTATTTTGTTACCCTGCGCTGAAAGTATTTTTTCTTTATTTAGTTTTCATATATTATTTAAATTTTCTGTTGGTTCTAGTGGTGCGTTGGTGTCACTTTTATTGTCCACTACGAAAATTAATTGGCTTGGCATTGATCTTTTTGCCATTGTGGCACAAGTTTGTTATGTTTATGGCTAAAGGCGATTAGTCAATTTGATGCTGCAGCTTTGGGGAATA

ATGAGATCATCGGCTGCCACAGGGAAAAAATCAACGCAAAAATATCTCTTGAAATTGGGAACTAATTGATGACAGATTTTATTCAGACCCTGCCGAAAATACTTATTACTTGTATTACTCACAAAGATGAAATACTTGGACTAACTCAAGTTTTGATTTCAACCAACAGATGGCGCCAGCTCAGCAGTATTGGGCGTTTTGAAGGTCGATCTGTGCCTGGAGGACAACCATGAGGAGCACCATACGAGTCACTTCTATGCGGCAGCTGCCAAGGAAGCGTTGGTCGTGCGACGAGGAGAACCCTTCCGGCTGAAGATCCACTTCAATCGAGACTACAGTCCCAGCAAGGATGCCATCAGTTTCATTTTCACAGTAGCGGATGACACCAAGCCGAGTCCCGGTCATGGAACACTCAATGCCCTGGTGCCGCACGATGGCATCGATTATCTGGGCGACACTTTGGAATGGGGAGCCGGCATCGAATCGCATGAGGGTCAAACGCTTACGGTGCTAATCAAGCCACCATCCACCTGTCCCGTAACGGAATGGAAACTGGACATCGACACGAAGCTGCTGGGCGATGGATCGCGAAGCTATCCCCTGCCGTTGCCCATCTATGTACTCTTTAATCCCTGGTGTCCGGATGATCAGGTGTATCTGGAGGATCGTGATCAGCGCAAGGAGTACGTGATGCACGATACCACGCTCATCTGGAGGGGATCGTACAACAGATTGCGTCCATCGGTTTGGAAAATCGGGCAATTCGAACGACATGTGCTCGAGTGCAGCCTAAAGGTATTGGGTACGGTGGGCAGGATTCCGCCAGCCTACAGAGGCGATCCCGTGAGAGTTGCTCGCGCCTTGTCCGCACTGGTGAATTCGGTGGATGATGACGGTGTTTTGCTGGGCAACTGGTCAGAGGACTTCTCCGGCGGCGTGGCACCCACCAAGTGGACTGGGTCCGTGGAGATTCTTCAGCAATTCTACAAGACCCAGAAGTCGGTGAAGTTTGCACAGTGCTGGAATTTCAGCGGTGTTCTGACCACCATTGCCAGGAGTCTGGGAATACCGTCTAGGATCATCACTTGCTACTCCTCCGCCCACGATACACAGGCCTCACTCACCGTGGACGTGTTCATCGACGCGAACAACAAGAAGTTGGATGCAGAGACCACGGACTC

 

---

Search Again