CR00409

CRIMIC CR00409

Gene Mtpbeta

CG4581

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:23868373..23868373	60A5	FBti0200552	83173

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	GCGGAGCTTTGGACATTTCCCGACAAAA	GACCTTCTCGTTCAGGCCCAAGTAAGT

Images

Construct Design Sequences

sgRNA sequence	TGATAATTCATAACGATTTGTGG
sgRNA2 sequence

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CCAGATCGGTGAAGTAGCCC	CAGCAGCAGCCGATTGAA	TGCTGCCACAAATCGTTATGA	GAGCGCAGATGGACATCGTA

Amplicon 5'	Amplicon 3'
CCAGATCGGTGAAGTAGCCCTTCTCCTGGGCCTCCTTGGCCAGTGTGTGGGATCGCAGGGCGTACTCGTCCTGCTCGCTCCGGGATACGTTGAAGGCGGATGCCAAGCGATCGGCCGAGTGTCCCATGGTCTCGCCGGAGGAGAACTCCGCAACGGCGGGCAGCTCGGGGGCCAGGAAGTCTGGCCTGAAGGTGGACAGCAGGGCCAGCCGCTGGCCCAGGGTCTTTGCCTTGTTGGCTTTCAGGAGCAGGGAGCGCATCTTGCGGGAGTGGCGAATGGGCACATCGGACATGAACTCCACGCCGCCGGCAACGATGACATCGTAGGTGTTGGTGGCTATCAGACCCATGCCCGTGGTGATGGCCTGTAATACATGGGCTATAGATTCAAGCTCCGAGGAACTTTGGGCGAGGCTCTTACCGCATTGGAACTGATGCAGGCCATGGTGACGGTGTGGGCAGGTGTCTTGTTGCTGAATCCAGCTGCCAAGGCTGCCTCCCTGGCAATGTTGGAGGTCTTCACCTCCTGGATGACGCTGCCGTACACGATGTAGTCGATGAGCTCCTTGTCCAGGCGGTTCTTTTGCAGCAATGACCTGTTGAATGGGGCGGGGTAAGCTAATACTCCAAGGATATTGGGGATTGTAAACCTACAAAAGCGAGTGCCTGGCCAGTTCATGGGGCATCAGCTTGGAGTAGGTGGTTCCGGAGGTCAGAAAGGGTGTGCGGACTCCATCCACGAGCACGATATTCTGGCTGTTCTTCTGCTTCGGTTGTGGATTCGCCACAGCGGCCGATAGAGCGCGGCATTGAACTGAAAAAGGGTTCCGGATTCGGGATTATCCTTAGCACTCTCGTTTCGCGTTATTTTTAATCATGATGTACCCATACATTATCTTTGTCCCGCAAGACGTCACTGCGAATTCGAGTTTGGACTTTGGCCCTTTCAGATTCAATCGGCTGCTGCTG	TGCTGCCACAAATCGTTATGAATTATCAGAGCCAAAGTCCAACTGGTGCAGCCCGTGTTTGGAATGGGGCATACTGCAACACCCGCACTATTAACTCACCTTTCCTGGCGACATTCAACAGGTTGAAGGCGTTACCCTTGCGGCAGACATTTTGAAGGCTCATCTTGCGAAAGTTTTCTGATTTCTTTTCTCGCTTTTCTCGTTGTTGCGACTGCAGCTGTTTGGCTAGTGATGAACATGAACGCCGGATCGCGGCGAACTATCGGTTATCGAAAGCTGATGCATGAAAAGCTTTCGAACTGTAAATACCCTTTCTGAGGATCTTACTCGTAGATCATAAAGCATGACAGCTTGTAAAATTCGGTAGATCTTCATTTCCAACTTTAGATATATTTCTTGGTTATATTTGAATATACAGTTCTAAATTTTTTAGCTTCTGAGAATCGTTTACTATTCCTACACCATTCACCTTCATTCAAATGTATGCAACGCAATCTGGGAGTCCCATAAAGGAATAGATTTCTCCAATGACCTTGATCTTGATTATCCTATTAAGCGTGCTGTTTACGATGTCCATCTGCGCTC

Amplicon 5'

Amplicon 3'

CCAGATCGGTGAAGTAGCCCTTCTCCTGGGCCTCCTTGGCCAGTGTGTGGGATCGCAGGGCGTACTCGTCCTGCTCGCTCCGGGATACGTTGAAGGCGGATGCCAAGCGATCGGCCGAGTGTCCCATGGTCTCGCCGGAGGAGAACTCCGCAACGGCGGGCAGCTCGGGGGCCAGGAAGTCTGGCCTGAAGGTGGACAGCAGGGCCAGCCGCTGGCCCAGGGTCTTTGCCTTGTTGGCTTTCAGGAGCAGGGAGCGCATCTTGCGGGAGTGGCGAATGGGCACATCGGACATGAACTCCACGCCGCCGGCAACGATGACATCGTAGGTGTTGGTGGCTATCAGACCCATGCCCGTGGTGATGGCCTGTAATACATGGGCTATAGATTCAAGCTCCGAGGAACTTTGGGCGAGGCTCTTACCGCATTGGAACTGATGCAGGCCATGGTGACGGTGTGGGCAGGTGTCTTGTTGCTGAATCCAGCTGCCAAGGCTGCCTCCCTGGCAATGTTGGAGGTCTTCACCTCCTGGATGACGCTGCCGTACACGATGTAGTCGATGAGCTCCTTGTCCAGGCGGTTCTTTTGCAGCAATGACCTGTTGAATGGGGCGGGGTAAGCTAATACTCCAAGGATATTGGGGATTGTAAACCTACAAAAGCGAGTGCCTGGCCAGTTCATGGGGCATCAGCTTGGAGTAGGTGGTTCCGGAGGTCAGAAAGGGTGTGCGGACTCCATCCACGAGCACGATATTCTGGCTGTTCTTCTGCTTCGGTTGTGGATTCGCCACAGCGGCCGATAGAGCGCGGCATTGAACTGAAAAAGGGTTCCGGATTCGGGATTATCCTTAGCACTCTCGTTTCGCGTTATTTTTAATCATGATGTACCCATACATTATCTTTGTCCCGCAAGACGTCACTGCGAATTCGAGTTTGGACTTTGGCCCTTTCAGATTCAATCGGCTGCTGCTG

TGCTGCCACAAATCGTTATGAATTATCAGAGCCAAAGTCCAACTGGTGCAGCCCGTGTTTGGAATGGGGCATACTGCAACACCCGCACTATTAACTCACCTTTCCTGGCGACATTCAACAGGTTGAAGGCGTTACCCTTGCGGCAGACATTTTGAAGGCTCATCTTGCGAAAGTTTTCTGATTTCTTTTCTCGCTTTTCTCGTTGTTGCGACTGCAGCTGTTTGGCTAGTGATGAACATGAACGCCGGATCGCGGCGAACTATCGGTTATCGAAAGCTGATGCATGAAAAGCTTTCGAACTGTAAATACCCTTTCTGAGGATCTTACTCGTAGATCATAAAGCATGACAGCTTGTAAAATTCGGTAGATCTTCATTTCCAACTTTAGATATATTTCTTGGTTATATTTGAATATACAGTTCTAAATTTTTTAGCTTCTGAGAATCGTTTACTATTCCTACACCATTCACCTTCATTCAAATGTATGCAACGCAATCTGGGAGTCCCATAAAGGAATAGATTTCTCCAATGACCTTGATCTTGATTATCCTATTAAGCGTGCTGTTTACGATGTCCATCTGCGCTC

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