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CRIMIC CR00412

Gene Gyf

CG11148

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	4:865026..865044	102E5	FBti0199582	79256

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	short band	GTGTCTGTATGCGTCCAGATACTTACG	TCGTTTCCACTATCTGGATCAGACAGG

Images

Construct Design Sequences

sgRNA sequence	TAGACCAGCTGAAATCCCTATGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AGGATCTTCATCACGCCAGC	CAATGCATCAGGCCTTGTTT	GGATTTCAGCTGGTCTATTAGCA	ATATGTCAGCCGAGCCTTCG

 

Amplicon 5'

Amplicon 3'

AGGATCTTCATCACGCCAGCTTGTAGATCTTCCTAAAAAAAATTTTTTTTTAAGTTCTATACTGTTGTTCAATAACTTGGACTATTTAAATTTTAACTTGGTTCATCTTCTTAGCAAATATAGCAAACTTACTAAACAGAAAACCGCTATAGTCGAGCTATTGCAAAACTGTTACCCATTTAGTTAGATACTAGAAATGCAAGCGATATTCAGATATACAAGTAGGAAAGCAAATATGTCAGTGATTGTACACCTGATCCACATAGCGGCAGAACACTAACTCTTAATCACTAAGCACGACAAAAATTTTATAAATACATTATATATACAATTTATTATAGTATCCAAACTTCGATAGAAGATTACCGGGTGAAGAAGTACATTTTAATAAAGCGTAAAGCGTATCAAAGTGTAAAAAGTATATTTGGTTACGTTGTTATGTATAGGCAAGCCCATATTTGAATAGTTTGAACTGTTAGTTAGTTGGACTTTGATACATAACACTTAAAATGGGCTCGCGTTTTTTTATTTTTCAATTTTGATAAATCAAGTCTATTCTGGAAAAAAAAAGATCATTATTTGAAAACGTACTAAAGCTACCAGCGATGTACTTTAGGAATGAAATAAAAACAAGGCCTGATGCATTG

GGATTTCAGCTGGTCTATTAGCATTTTTGTTTAAATATTCTCATATAAAATACTTCATTAACTAAAACATTTAATCTTAATTTTAATATATTTATGAAAAGGTTTTTTAGAATACGTTTTAAAGAAACACAAACTTTACAACAGATTGTAGCATGAATGGTATCGAATGAATAGCCAACTATATAGGCTGGGGAAAGTTTTGGAAAGTCAAATTTACATGATAGAGATAGCAGTACATGTATTATTGTGCTCATATGTGTAGGAGTAAAAATGAAAAATAATAATATAAAGGTAATTGACTGACCCCACCGATGGCTATTGGTACCAAAACCTGCGTTTGTACTTCCACCGACGACACCGCTCCGCCAACCAGCAATATCGGGTCCGCTCATGGAACCGCTGGAGGATGGACCATCACCGGATCCATCTTCGTTACGTGTTCGTCGCCAGTTTTCCAAGTTGCGATGATTATTAGAATAATCTTTTCGAGGACTTGAGCTTGGAGTTCCATTCCAATCTAGAGCACCAGAGCCACTAGTGGAGGTGGTGGTTGCCGCAGAATCTCCAGTTCCGTTGCGGTCGCTCCATGTGCGTTCGGCCTGGCAGAAAAAATCAACAACTAATCGTACTTCCTTATTTAGTTCGTATTTGCAATTACCTTTGATATAACCGATAAGCTTTCATCATATAGACCGCTTGGGCGTTGGTATATCGGATGATAGGCCGATTTTCCGCGCATCCGTCCCCGGTCAACGGTTCCACCACGTCCAGAGCCTCGGGAGGCACTACCAAATCCACTGGGCGAGCGCTGCAACCAGGCAGGACGAGACTAGTTTCAAAGAAAAGTAAATTATGTTCTAAAACTCATGTCAAATTACTTCACATACGTTATTGCCAAGTGAGTTTTGGTTGATGTCCTCCTCGGAGCTCGGTGTCAGTGCAAGAGGGCACTGAACCTTTTCCACGAAGAGCTTTTTGAACGATGGCAGGATCTGCGGCAGTAGGCAGTTCCGATCGAACAAGGACAGCATTTCCTCGCGTCCGTACCGGTATTCTGGAAATAGATTACGGGAAGCGGATGCCGCCGTGTTGTTTCCCAGGTTGTGCCCTCCAATCGAATTGTTTTGAGCTGCATTACCAACGTTGTAGGTACTGGGAGAGCCCGAAGGCTCGGCTGACATAT

 

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