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CRIMIC CR00413

Gene prom

CG42310

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:24425159..24425185	60C8	FBti0199366	78905

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGGGACGCCACTTAAATGGAACAGAACC	CGCTCATCAGAGCAATGAAGCCTGACT

Images

Construct Design Sequences

sgRNA sequence	TAGGCATTAAAAATGCATGTTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TGGGTGAGATTGAAGCGTCC	GGCGAGAGTCTGTGGCTG	GGTCAGGGACGAGGCTAAGA	CGATACGGCTCAGCTCCATT

 

Amplicon 5'

Amplicon 3'

TGGGTGAGATTGAAGCGTCCTGCCACACGACTCCCATCCGCCATCCTCAACTGCACGGTGGTCGTGTCGGCCCGTTCGTTGAGCTGAAGGCCTTGGGCTTCAACGGGCATTGGGCCTACGACGAGTATCTGGGGCGAGGGGCTGTTCAGCGGTCGACCTGGTCCCATGAACTGTTTCCGGGACAAGTGACGATAGGATTCGTTAGTGTGATCCTGCACGCTCAGCTGGACTCTGGGCACCCGGAGCATTTCCTCGGGGAAATCCCCCCTCAAAATAGCGCGGAGAAATCGCTCGTTTTCGGGCAGTGCATATAATCTCAACGAGCCGTCGTCCAGGGAGAATCCCTCAGACCAGAGGTGAAGGACGACTATGGTGTGCTCGTCGTCGGCCGGCTCCGTATCCGAATCCTCGGTCGCGGATCGAGGAGGCGGATTGATGGGATGGGCGCTTCCCAACCGATTGCCATGACCCCAGACCCGCAGACTGCGGTCGCTGTCGTTGTTGGTTATAGCCGGCGTGGAGCTGTTGATGTTCACCCGCTTGCCCGCCGGAGTGTCATGATCGGTGGAGCTGTCCGATGCACATGCCTGGTAGCCATCGTGGTCGCCCTCCTTCCGCCGGGATATCTCACTCAGCAGGGAATGGAGATCCCGATTGCTCTCATTCGCTTGCGAGTGCTCGGAGGACTTCTCGGGCTCTTGCTTTTTGGAAACCGCCTCCGATTCGTAGGTGCTGCTGGCTACCTCCAGGGACCAGTTGTTGGAGCTCAGATACTGACGGGCCACCTCCTCACGCACCCCATGCCGTTTCATGAAGGTGCTCAGCTTCTGCTCATCGGTCATCTGGGCAGGGACGAACAACCCTGGGGCTGTTTCGTCGGATTGAATTTAGAAACACATATCGTCCGAAGGGGGATCACTGTAGCTAGGCCGACTGGGGCTTTGGCTCGTTCCGAAAGTCCTTGTGGCAGCCACAGACTCTCGCC

GGTCAGGGACGAGGCTAAGACTACTAAGGGATTAGTGCCATTGAAACTATTTAATTCAAGCTTGTTTTAAACGTAATACCAAGCCCTGTAGCACTTCGAAAAAATATTTTGTATAGAAAACTATCAGAAAGAATTTTATTAAAGGTCCTTTGCTAAAATAAAATATTAAAGTAGTTTTGGGCGAATGTAATAGCTATCATAAGGACACCACTAATAAAACATTTAAAGAAAATCCGAATACGAATCGACATAATTTATGCGGACACAAGTTTTTCCCTGTGCACAATTTATATGAGGTCTTCCATCTGGGTTGAATAACTTGCCCACCTATCAAAGGCTCTCCTGAATTTACATCTCTAATGCCTTCATTGTTGGCAAGCAGACGTTCTGAATACCAGCCCACACACCTGGAAACCCTTTCCCCGATATCATTCGATCCTACACGGGCACAAGGATGCAGGACCACGCCCTCTGCGAGGGGCGGACATGGAGGGACTTACCCAGCGGCAATTCCTCGGGCGTGATAAGCGATAAAAATTCACTAACGTAGGTGAATATAAATGGTCCTGGCGCAATGTCCATGCCTGGTATGCTGTAGGTAAAAAATGTGGCTGGCGTCGTTGTTGGCGGCGCCGTTGTTGTTGTTGTGGCTAATGCTATTGTGGATCCGGTCAGGGCGGACGTGGTGGGTCCTCCAAGGGATGCGAAGCCCGCCGACATCGCTGCTGATGACGAGGACATAATTGTTGTTGCGGCTGGCGTTGGCAGTGTTAGATTGCGCTGGTCCATCTTCGGCCGGATGGCTTGGTTCTCCGCCCTGCGAATGAATTAATGCGGAACAGGCATGGCCATGAATAGTCGGGTAGAGCGCGGGAGCGCGGAGAGGCGGAGGGGCAGAATGGAGCTGAGCCGTATCG

 

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