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CRIMIC CR00415

Gene Nep1

CG5905

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	X:6100886..6100905	5D5	FBti0199583	79257

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTTGCATGACCCAGGAGTGCGTGAGAA	ACATTCACTAGCTGCGTCTCGAAGAGC

Images

Construct Design Sequences

sgRNA sequence	ACCAAGGAGGAGCCATTTCCCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TGCTTGGCATGCCGTTAGAT	GCATTGTTCACTGGTCGTGC	GGCTCCTCCTTGGTAGTTCA	GTTGTCGCGTTCATCGATGG

 

Amplicon 5'

Amplicon 3'

TGCTTGGCATGCCGTTAGATACACGGACACAGAAATGTATAGCTACATAGATAGTTCGTTGGATAGATAGTTGGTTGGTTGGAGGGATTGGCATATAGATACACCTGTCCAGTTGCAGGGCATATCCTGAAATATACATGGGCACAATTACCAGGCGATGCCATCGATTCGAGTGCTCCACTTAGCATACTTTTCGGGGGTTCACCCTAATTGGTAACCCAAATCAGTTGCTTAATTGAACTAAGCAAAAAAGGGGTTTGGCTATTTGTCATATTCTCTAGATAATAACACCTTCGAGGAATCGAGTAATGAAATGACAGATTGTTGGCCTTCAAGTTATTGAATTAGGGAAATTGCTTAAGTGGATGACAAATGAGTGTTAAGTCCGACAGGTAACTTCAAATTTCCAGAATGCAATTTTTGCTGGCAAGCTAGTATCCTTTGGCTTTGTAGCTACAGCATAAATCAAGGACGTTTCTAGAAGTAAGAAAAATCTTGAAGGTGGGGGTGGGGGGGGGGGGCACACTAATGAGTTTCGCACTTAGTTTTCCGAGATAATGAAATTCAGCTTAATGACGTTGATTTATGGATTGGCAGCACGACCAGTGAACAATGC

GGCTCCTCCTTGGTAGTTCAGTTCCGCATTCATTCATAACTTTTGTGTGACCGAAAAGTTGCATCGGGGAAAAAAGTGTGTGAATTTTGCGGTGGTGAAAACTACTTTGCTTATCGCTTATCGCAAAAGTTCCGGTGATGATTTGTGGTCCAAGTTTATGGCCGGTGCGATCAAAAGTAGGCATAAATTATGACCTCAACGGGTTGAAGCACTAATTGCACTCCAGCTGAAGAGTTTCCCACCGGGTTGCGTTTGATTTACTGCACTGAATACAGTATTCAGTTTAACACACAAAAAAACCGGGTTTAACACACCAAAAATTATCTAAATCCAGCCGCCTCCCTGCTCTCGGCCATGGACCTCAACTCCGATCCCTGCGAGGACTTTTTCCAGTACGCGTGCGGCACGTGGAACAAGATGCATCCCATTCCCGAGGACCGCAGCTCCATCAGCACGTTTGAGGTATTTATTTCGAGATTGGGATATGCCTCCGTAAGATTCGGTTTTCTAAGTGCTTATCCTAATTTCACGCAGGTCCTGTCCGATCAGCAGCAGGTTATCCTGCGCGCAGTTCTGGAGGAACCCATCGATGAACGCGACAAC

 

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