CR00419

CRIMIC CR00419

Gene lz

CG1689

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	X:9293271..9293284	8D5	FBti0199367	78906

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGAGATTGAGATTTCGAGAGATTTCGAGGGG	AACCGGCTGCACTTTTCAAACACCTTC

Images

Construct Design Sequences

sgRNA sequence	AAGGGGGCCAGTGAAATGAGGGG
sgRNA2 sequence

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ACAAGTAGGACTCGCTCCCA	ATTTCACTGGCCCCCTTTCC	CGAGCGAAAGACAGGTTGCA	TACGGTTACGATGTCAGGCG

Amplicon 5'	Amplicon 3'
ACAAGTAGGACTCGCTCCCAATGTCTGGTCACAAGCCCAGCCACCCACCTGGAGGTGAAACCCTCACTCATCCGGAGCGAAAAGATAGAAATGGACTTTATCGATGGGGTTTGGGGTCTCCGGTTCGAAGTTCTGGGTTTGGGGTGATGCGACTGAGGTTTCTGCGGTCAGATTGGATGGGTCTCTGTCTCCTTCGGGCTTTTGGATCCCGTCCTGGTCCTGCAACAACAATGTCGCTTTGTTGGTGTTTTATTATATGTATGTACATACATACATATATGTCGATGTATTTGCATTGCCAGTTTTACTGACCAGACAGACAGACCGGACCGATGGACAGACGGAATGCTAAAATATCGAAGTAAAAAACCGAAATTCATTTCACATTTTCCCAATCGATAAAAGCTGCCGATGGCTCGCAGTTGCAGTTTGCAGCCGCTTCAAACGGTGTCTAGTTTAATGGCCATAAATAGTGTAAACATTCGGTCAAGCCCAGAAGAAAAAAAAAAACAAAAAAAAGGTTGGTATAACTCTTTTTGGCCACAATGTTGCCCGTTCCTCGGTATGCAAATGTGGAAAGGGGGCCAGTGAAAT	CGAGCGAAAGACAGGTTGCAACATTTCCATATTTTATGTGCACTTTAAAGACTTTGGCGGTAGTCATTAAGGCGGGATAAACCAAATTTATGTCAGTGGTTCGAACAAACTGCACCGCCGTGGCAGTTTTGGGGCTTTCAAGGGTTAATCGAACTGCGCGATGCCCTAAATGCTATTTTATGTTCATTCGAGCATTTAAGATCGCAAACTAACACTTTTCACCCAATTTAGTTTGGCAAATAAACTAATTTCCCTTGCCATATCTTAACCGTATCGATTGGTACCCTATATATGAAACATCTATATGCATCGTATCGACCACATACCAAAATTCATAATAATAACTCAAGGGGTTACCGAGTTCACTCCAGACCACAAGCGCTCACACTTTCGCCCAAAAACCCAAAAGAAGTGCAGCCATTAAAGTTGAACGCGGTGACGCGTGTTGCACGGACTGGACTGGGCTTTTGGTAATTTTGGAATCGCAAATTGATTCGGTAGCCCAGTGAGGTCGTCGCATAAATCCTTGCCAGACATTTACGACTAAATTCTCGAATTTGCACCGCTCTCTAATGACGTGCCGCTTCTGATGCTTTCTTATTTTGTTTTTTTTTTTTTGCAGGGAAGAGCTTCACGCTGACCATCACGGTGGCCACGAGTCCGCCACAGGTGGCAACCTACGCCAAAGCCATCAAGGTGACCGTCGATGGACCCCGCGAGCCCCGCTCCAAGACAAGTAAGTGTTGCAGATCGTATAATCCTGAAAAATATGGTCTTAGAATTAGCCATTAACTATGTGAGATGTGCTTGCGATTTCCTGTTGTTTGGCATCGAATGGAATGCCGTGTAGGATAGTAGTAGTTTTTTTCTTTCTGTGCAGCAGCAGCAGCAGCACCCTTCTCAAAGGTGTAAAATATTACAAGCAGTCATATGTAAGTGGCTGCATTGTTTTGCCAATTAGCAATTATCGCTTGCCACAACAACAAAAGGAAAAGCGGGGAAAAACTGATGGAAAGCCGTCCCGCTGCGTTTTGGGTTACCGATTGCAACATGTGGCCATGTCCGCTCTTCCCGCCTGACATCGTAACCGTA

Amplicon 5'

Amplicon 3'

ACAAGTAGGACTCGCTCCCAATGTCTGGTCACAAGCCCAGCCACCCACCTGGAGGTGAAACCCTCACTCATCCGGAGCGAAAAGATAGAAATGGACTTTATCGATGGGGTTTGGGGTCTCCGGTTCGAAGTTCTGGGTTTGGGGTGATGCGACTGAGGTTTCTGCGGTCAGATTGGATGGGTCTCTGTCTCCTTCGGGCTTTTGGATCCCGTCCTGGTCCTGCAACAACAATGTCGCTTTGTTGGTGTTTTATTATATGTATGTACATACATACATATATGTCGATGTATTTGCATTGCCAGTTTTACTGACCAGACAGACAGACCGGACCGATGGACAGACGGAATGCTAAAATATCGAAGTAAAAAACCGAAATTCATTTCACATTTTCCCAATCGATAAAAGCTGCCGATGGCTCGCAGTTGCAGTTTGCAGCCGCTTCAAACGGTGTCTAGTTTAATGGCCATAAATAGTGTAAACATTCGGTCAAGCCCAGAAGAAAAAAAAAAACAAAAAAAAGGTTGGTATAACTCTTTTTGGCCACAATGTTGCCCGTTCCTCGGTATGCAAATGTGGAAAGGGGGCCAGTGAAAT

CGAGCGAAAGACAGGTTGCAACATTTCCATATTTTATGTGCACTTTAAAGACTTTGGCGGTAGTCATTAAGGCGGGATAAACCAAATTTATGTCAGTGGTTCGAACAAACTGCACCGCCGTGGCAGTTTTGGGGCTTTCAAGGGTTAATCGAACTGCGCGATGCCCTAAATGCTATTTTATGTTCATTCGAGCATTTAAGATCGCAAACTAACACTTTTCACCCAATTTAGTTTGGCAAATAAACTAATTTCCCTTGCCATATCTTAACCGTATCGATTGGTACCCTATATATGAAACATCTATATGCATCGTATCGACCACATACCAAAATTCATAATAATAACTCAAGGGGTTACCGAGTTCACTCCAGACCACAAGCGCTCACACTTTCGCCCAAAAACCCAAAAGAAGTGCAGCCATTAAAGTTGAACGCGGTGACGCGTGTTGCACGGACTGGACTGGGCTTTTGGTAATTTTGGAATCGCAAATTGATTCGGTAGCCCAGTGAGGTCGTCGCATAAATCCTTGCCAGACATTTACGACTAAATTCTCGAATTTGCACCGCTCTCTAATGACGTGCCGCTTCTGATGCTTTCTTATTTTGTTTTTTTTTTTTTGCAGGGAAGAGCTTCACGCTGACCATCACGGTGGCCACGAGTCCGCCACAGGTGGCAACCTACGCCAAAGCCATCAAGGTGACCGTCGATGGACCCCGCGAGCCCCGCTCCAAGACAAGTAAGTGTTGCAGATCGTATAATCCTGAAAAATATGGTCTTAGAATTAGCCATTAACTATGTGAGATGTGCTTGCGATTTCCTGTTGTTTGGCATCGAATGGAATGCCGTGTAGGATAGTAGTAGTTTTTTTCTTTCTGTGCAGCAGCAGCAGCAGCACCCTTCTCAAAGGTGTAAAATATTACAAGCAGTCATATGTAAGTGGCTGCATTGTTTTGCCAATTAGCAATTATCGCTTGCCACAACAACAAAAGGAAAAGCGGGGAAAAACTGATGGAAAGCCGTCCCGCTGCGTTTTGGGTTACCGATTGCAACATGTGGCCATGTCCGCTCTTCCCGCCTGACATCGTAACCGTA

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