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CRIMIC CR00420

Gene Rtnl1

CG33113

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:4995263..4995291	25B10	FBti0200554	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	short band	ATTTATGGAGCGGAGCAGTGATTGCTGC	CCTTCAGCATTAGGCGCGAGTTTAATGAC

Images

Construct Design Sequences

sgRNA sequence	GCTAACGATAATTACCACCTCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GTCCTTCTCAGACTCGGCAG	GATGGTGCCACAGTTCTCG	TTGTTCAACGGCATGCAACT	CCAAGCTAAGGGCCTCAGAC

 

Amplicon 5'

Amplicon 3'

GTCCTTCTCAGACTCGGCAGCGGCCTCGGGCTTCTTGTTGCCAATGGGGATGGCCACTCGGATCCTGGAACACAAAAGCGATTTAGAATATCTTTCTTACGGAGATTTTAAATGGTTAAACTCACTTGTCGGTGATTTCTGTCAATTTGCTGCGCACCAGATCCAAGTGAGTGTCGATCGATTGCTTGTTGTTCTCGTAGACCTTGGGCAAGGTAAACAGCGAGACAAAGGCTGCACAAATATAGAAGATAATCAGTTCTGTTTTCGATTGGTTTAAGTTAATCCTCCACTTACCCAAGATGACCAGAGTCATGCCATTGAACCAGGCACCCACGTAGGTGAAGACCCACAGAATGACGCCGAACTTGATCGAATCGATGATATCCTCAACAAGAAACAGACGCCTCAGCTCGGAGATGAAGCCATTGATATGTGCCACAGCCACGCCGGCAATGTTCTGTACCTTTTCGTGCGACAGCGTCAGATCCAGCTCCAGGTAATCCCTGATTTCGTAAGGAGTTACGTTAGTTAAACTTTTTTTTTTGTTGGGAGACATCAGTCTACAATACTCACTTAAAGGGGTGACCCTCGTTTGTCTTTTGCACGGCCTGTGTCACAGATTTGTAGATTCTGAAGGCGACGGTGCCGAAGAGGGTTAGGAGCGACAAGTAGGCGAACACGCTGATCACCGAGAAGCTGGAGATGGCCGCCAGTGTGATCAGGCCAGCGCCGAAGACAATGCCGGATTTCTTCACATCGCGCCAGTAGATAAGGGATTCCACTGGAAAGAAGAGCAAATGGATCAATTAGAATCTGCTTTTGTCCACCATACACATTTGATTTTTCATTCCCAAATTATAACCCTCATAAACTGTGAAAAGAGACGAGATACGAGTGGAAAATGTGGACTGAGAATCCCCAAATATACGAAGCACACATACGTTCAGCGTCTCGCACGCATCTATGTACATATATCTTTTCAGCTGGCGGCAATTATGCAATCTATGAGATAAATAGATGAGATACAGATTCGAGAACTGTGGCACCATC

TTGTTCAACGGCATGCAACTAAGCTCTGATACTATAATATCTGAACCCAAGCACCCCCTTTTCTCAGCCCGGTTGGTTCACTCACACAATCTGTTCGACATTTTTCGGGTATTCAAGTTTTCGGGTTTTCCAACGGATCGTTCGCGGTGCTCCTACGTTTGACGTTCGCTGCCAAGTTCTACACACTAGAGAAGTATTATTTGGCGTCTGCTGGTCGGGGGCAGAACGTATATGAAAATGTCTAGAAGAAGAGGGCCAAATTGTCCGGACCCAGCCGGAAAACAAAAGAGAGCCGTAAAGAGCATAGCTACCAAGTGGAAAGAGCGATGGCAAAAGAGTGTTTGAGTGGCGAACGGATATGGAAATGGCAGTCGCAAACGCTTATAAATCGATTGTCCAGCTCGTGCCGGAATGTATCTCTTGGATTCGGATTCAGTCGCTGACGTAAACACCTCTCCATCTGCTATAACTTGGCCATCGGATCGGGCTTCATTCGCTTTCGTCGGATTTGCCGATAAGAAGTCACTGCCCCGCGGCGTCTGGGTTAACCCAATTAAGATATGTACTAAAACAAACAAATCGTCTATAAATACACATCTCAAGTGTTGGAAATAGGGGATTTGCCAACTCATTAAGTGGTCATTAGTTTGAACTGATTTGTTAGGTTGCTCAGGTAGCAAGCTACTTAGGGCAATCCAGTAAGGTGCTACCCGCAAGGAAATGATATATGACCAATAATTAACGACAAGCATTTATTATTTTATAGTACCATTGGGTTTGGCCAACACATTAATATGCTTATGATAGTATGCTTGCTTGGAGATTAATTGAAATCTATATATTAATCCTCAATAAAAATGGTATATAAATAGTATAAATGGTATATCACTTTAGAATACACCTAAACAAATAGCATATAGATAGATATACGAAAAGTCTGCAAGCTGGGTAAACTAGTTTCAGATTCGAATTCGTCTAAGTTGCCAGGCCAGACTCGATCTGAGGGGTATATTATGTAAATATGGGTGAAATAAATTAGAAAGGAGACGCACAATAGTGATGAGATGGCAGCGTCTGAGGCCCTTAGCTTGG

 

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