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CRIMIC CR00421

Gene CG32751

CG32751

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	X:6197780..6197802	5D8	FBti0199368	78907

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTCACGTGTCATCGATGATAAGTGGGTT	CTGTTCCCACTCCAAAAGTGCACTCAT

Images

Construct Design Sequences

sgRNA sequence	GTCGAGTGATTGCTAATTGATGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CCCTCATAGATGCCCACTCG	ATGGCTCTCACGAAACCCT	AGTTAACGGGCCCATGTCAG	GGTGGAGTTTGAGCCATCCA

 

Amplicon 5'

Amplicon 3'

CCCTCATAGATGCCCACTCGATAGCTGTAGTGACTGGTGTTCCTCGAGGGTTTCCCCAAGGATCTCCAGGCCAGATCGAAGTGACAACAGAAGCCACCGTGACACAGATTCTGGCTAAGATTACCGATCTTTCCAGCGTCGATTTTCAGCTCCTCGCTGGTGAAGTTTTCCAGATAATCCCGTTTGATGTTGAAACTTGAACTGGTGGCCACTTGACGTTTGAGATCTCGCTTCATCCGCCTTTTGGCCCTGTATTTGGGTACCCGGGCCACATATATACTTCTCTCACCGGACTCCTGCCGCATCACACTTCTTAGTGAGCCACTGCGTCCGTGATAAATGCCCGATCCACTATTTCCAACAATGGGATCACTTGCTCCAGCAGCCAGCAAATTCACATTGTTGGCATAGGACCAACCCAGCTGAATTTGAACAGCTTTCGAAGAGAAAATACCATTAACTATTAAGTACAAATTATCACCATTGAATTGGCCACAGGTTTAAATTTTCCAAGAGCAGATTGCAAGCATTAACCACTTGAACATTCCGAATGGAGGACACTTTATCTCAAGGGTTTCGTGAGAGCCAT

AGTTAACGGGCCCATGTCAGTGAACTGACCCACATTTTTGATTCCTTTCTCGACTTACCTGTGAGGAAGGGCAGTTGGGAGAACCACATGGCCGGATACACGAAGTCCGTAATACCCTGCTCCACGATGAGTTGATGGGCGGGAGTATAGAACAGGATGTCGAAACAGATAAAATGACCGAAGGTGACCCCAAAGTCTGTCTCGAAGGTGCTCAGTTCCGGCAGAAAAGTGCTGTTCTTGGGCTCACCATAGAGATGAACCTTTCGGTACCTGGACACCACCACTCCCTGACGATCGAACACTACATTGGTGTTGAAAACATTCAGTCCGTTAGATGCACATGGCCTCGTATCCTCGGGAATATCCTCGCACTTCTGCTTCTCGGTCAGATTGATCACAATGTATTTACTGGCATTGCGGGCCGCACACGACAGGGTCACCAGAAACTCCTCATAGTAGGTGGCATTGGGATCACTCAAACAGGGGTTTATCTGATCCTCGGGATTGGGCACAAAGGTCGTGGAACCCGCGGAGTTCAGTGTGCTCTCGGGGAACACAATGATGTCCGTAGAAGTGGCATTCTGCGACTGGATTATCTCCAAATAGCCAGCTAGATTGTCGGACAATTCATTGGATGGCTCAAACTCCACC

 

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