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CRIMIC CR00424

Gene kraken

CG3943

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:853088..853121	21E2	FBti0199369	78908

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACACAATTCCGACGCAAATTCCGACC	TTTCACCACGAAACTCGCTTAGCACTC

Images

Construct Design Sequences

sgRNA sequence	GGTGAAATCGCCCGCGGGCCAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CTGCGTCGCTTGTTACCTTG	CAACCAGCGGAAACTTCGGA	ACGCAGACCATTTCGTCACA	GCATATGCCGTCCCAGAAGA

 

Amplicon 5'

Amplicon 3'

CTGCGTCGCTTGTTACCTTGCCGGGAGCCACCCGCCTCTGGTCTTCTTCTTCGTCGGACAATCCTTCCAGGCGCATGGGGTCCACTTGGCGTGATCTTCCGGCGAAGGAGTGAGCTCCGCCTGAGGCGGCAGCAGTGGCGGTGGCCCTCATTACCGTGTTAATGGACCTGGCGGTCATGTCACCGCGCGAATGGACATCCTGTGCGTACGACTTCATGGCCGCCTCCTCCATCTTGCGGATCTCCGCGTCCATTTTCTGGCGCTCCCGCTCCGATTTCTCGCTGTTGCGACTGATGTCCGTGATCCGCTTGGCCACATTCATCTTGTGCCGCTTGCCACTCTCGTGGAAGGCGACGCTCTGCGGGATTAGGGGTCATTAGAGTGCCGAAAAGCGAGTTTATTCTATGGACTCACCGCCTTGTTGTCGCTCAGCCAGCATTTGCAGAAGTCGCAGAACTTGCGCTCGTTTGATTTCCAGTACTCCGTCCTGTTGGAATCGAGAGTTAGCTATGGCAAAGCCGGAGGATAATAACGCAGACTTACATGATGATTTCTCAGCTTAATTGTTTCGAATACAAGTAATATTAAATTTTGTTTGCAATCGCAAATGAAAACAATGTGCGGGAGTTGCCACCCAGTCGCGAATGGAGCTGCACAATAGCTCCGTATCTGACTTTCTAGCCATTCCACCGCCAAACCGACATATCTGTCAATAGCTATTGACAATCGAATTCCGTTGATCGGCAAAACTTTGCGACTTTAGTTAGTCGCTTTTTGAGATTCCCCGAGGCAGGAGCTCGCCCGATCCGCGTCCAGCATTACGGAGCACCATTTGCGAGCAGCAATTAGCCATGGGCCAGACACGCGTGGCAGCAACAACAGCGGCGCAATCTCCCGCGGCGGAACTTTCGCCAGAGACAAGTGAGTAAACCGGAGGAGGAGGTCGTGGTCAGGTCACCGACTCGCACGCTCTGCGCACCTGAGTCCGAAGTTTCCGCTGGTTG

ACGCAGACCATTTCGTCACAGCTTGCTTTGGTTTTAGTTGAATTACTTATGTGGATTCCCCCTGCTTTCAGATGGCCAGACGGAGGAGCCCCTGCAGCTGCTGGGCGAGGACAGCTGGGAGGAGTTCTCTATCGCGGTGCCCTGGGGAACCGTTGAGGGTGAGTCACCTGCCAGCCGTTTCCTAATCAACAGCATCGACCATTCATGGCAATCCGACGTGATTTTCGATGTGACGCGGCTAAAATATTTTGTTAGTTTCGACGCACTACAAGTGTTCTACTAACTGAGCCCAATAACGCTATCTCTTTGCTTGTTTTTTGAGTAATATTTTTTGGAAACATCACGCGATTCCCCTGAACGATTAACATACACGTGCTGAAACCAAGTTACATACATATGTACTAATCGATTTTCCCGCTTGCAGCCAAGTGGTGGGGCTCGAAGGAGAGGCAGCCCATCATCGCCCTGCACGGCTGGCAGGACAACTGCGGCAGCTTCGACAGGCTGTGCCCCCTGCTACCGGCGGACACCTCCATCCTGGCCATCGATCTGCCCGGGCACGGCAAGTCGTCGCACTATCCGATGGGCATGCAGTACTTCATCTTCTGGGACGGCATATGC

 

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