CR00431

CRIMIC CR00431

Gene sicily

CG15738

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	X:11892481..11892481	10F4	FBti0199584	79258

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	GGACAAATTTTCGCATTTTTTGTAAGGGGTACGTACC	CGAAGCGAGATAGCGATATTCCTGCAAC

Images

Construct Design Sequences

sgRNA sequence	GCGGAGTAACAGAGTCTGAACGG
sgRNA2 sequence

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TTTCTGACAGACCGAGTGCG	TGAATTTAAGGCGCCACGTT	CAGACTCCGTTCAGACTCTGT	AAAGAAGGGTCCGCTCCATG

Amplicon 5'	Amplicon 3'
TTTCTGACAGACCGAGTGCGATCTGGCAGCACCGCTCAGAACATATGTTCAGTTGTCTGGTGGAAAGAAGAAGCACAGATGCGGCGCCTGGTGCGAAATTGGAATTGCCGATTGTTATTTAACGCCAAATCCGGCCAAGAAATTGTCAGCCATGCCGGAATTCACCAGGAGGCCAAGACCAATGCGATTCCCAGGGAAAAGAAAGTGATTGAGGCGGACAAGAATGGATACGGGGCCAAACATTGCATGAGTCTAGTGGAGTAAGTAGCGCAAACTTGTCGATTGAAGTTATCCGTATAATTGAACATGGATCTATTCCGGATAACCAGGAAATACGACTACGAGAACTACCTGTGCACCTTGCTCCTACCACGGGAACTGCGACGTGCTGCCTTCGCCCTACGGGCATTCAATGTGGAGGTCTCCAGATCGGTTAGCGGCCATGTGAGTGGAGCATCGAAATTCCTTGGGGCTTATTTTTTAAGTTAGAATAACGTGGCGCCTTAAATTCA	CAGACTCCGTTCAGACTCTGTTACTCCGCATCAATATTATTGTAAGCATAAGTGGCTTATGTCCGCATCTCATGAATATTATTACCATTTCGAACGGTTTCACAAATCTCTTAATTTCTTATGTACACTGCAAGAGTATAATTTCTCTCCTTCCTTTAAAACGGATAGAGAAATTCGTAGAATAAGCAACACTTCGTAGTTTTTAAACTTCTTATTTCTGTCCAGTAAAATCGTTTATATAATGACAAATATCCTTGCAGCAAATTGAGCCGCAAATAGCCAAAATGCGCCTTAAGTTCTGGCACGACTCCATAGACAAGTGCTTTGAGCCGGATTCCCAAAGATCGTACGTGGAGGACCAGCCTGTCCTGCGCGAACTGAAGCACACGGTGGGCAGTCGCAAGCTAAATAAGGTATATTTGCGCCGTCTGGTCACAGCACGGGAACGTCCGCCCACGCATGCATTTGAGTCGATACGCGAGCTGGAGGAGTACACGGAACAGACCTTCTCTTCGCTATTGCTCCTGCTGTTGGAGGTGGGCGGAGTGCGTGACCTAAATGCTGATCATGCCGCCTCCCACTTGGGCAAGGCCCAAGGCATCGCCACCCTGCTGCGATCGATACCGCTGGCAGGACGTCAGCAGGCGCCCTGCATTCCGCTCGAGGTGCTGGTGCTGCATGGCGTCAGCCAGGAGCGCATCATTCGCTCTAAGAGCGACGACAAGGGTGTCGAGGACTGTATATTCGATGTGGCCAGTGCCGCCAATACGCACCTAAAGCTCGCCAGGCAGCTGCACGATAAGGTGCCGCCGCAGGTGCGCAAGATCTTCCTCTCCGCCGTGGCCACGGATGCATATTTGGAGCGCCTCCGCCGTGCCAACTTCCTGTGAGTTCCTTTGTTTTATATTGCTTTGTTTTATATTACGACAAACACAGGAGAGAACCAGCTTCTTTTTGTAGTTAAGATGTTATTTTTATCCAGTTATAAGTACGATTTGCCTTTATTGAGACTTCTTTATTTCAAAACCTAGCTATAGTTTGTTGGTTGTTCAGTATTCATGGAGCGGACCCTTCTTT

Amplicon 5'

Amplicon 3'

TTTCTGACAGACCGAGTGCGATCTGGCAGCACCGCTCAGAACATATGTTCAGTTGTCTGGTGGAAAGAAGAAGCACAGATGCGGCGCCTGGTGCGAAATTGGAATTGCCGATTGTTATTTAACGCCAAATCCGGCCAAGAAATTGTCAGCCATGCCGGAATTCACCAGGAGGCCAAGACCAATGCGATTCCCAGGGAAAAGAAAGTGATTGAGGCGGACAAGAATGGATACGGGGCCAAACATTGCATGAGTCTAGTGGAGTAAGTAGCGCAAACTTGTCGATTGAAGTTATCCGTATAATTGAACATGGATCTATTCCGGATAACCAGGAAATACGACTACGAGAACTACCTGTGCACCTTGCTCCTACCACGGGAACTGCGACGTGCTGCCTTCGCCCTACGGGCATTCAATGTGGAGGTCTCCAGATCGGTTAGCGGCCATGTGAGTGGAGCATCGAAATTCCTTGGGGCTTATTTTTTAAGTTAGAATAACGTGGCGCCTTAAATTCA

CAGACTCCGTTCAGACTCTGTTACTCCGCATCAATATTATTGTAAGCATAAGTGGCTTATGTCCGCATCTCATGAATATTATTACCATTTCGAACGGTTTCACAAATCTCTTAATTTCTTATGTACACTGCAAGAGTATAATTTCTCTCCTTCCTTTAAAACGGATAGAGAAATTCGTAGAATAAGCAACACTTCGTAGTTTTTAAACTTCTTATTTCTGTCCAGTAAAATCGTTTATATAATGACAAATATCCTTGCAGCAAATTGAGCCGCAAATAGCCAAAATGCGCCTTAAGTTCTGGCACGACTCCATAGACAAGTGCTTTGAGCCGGATTCCCAAAGATCGTACGTGGAGGACCAGCCTGTCCTGCGCGAACTGAAGCACACGGTGGGCAGTCGCAAGCTAAATAAGGTATATTTGCGCCGTCTGGTCACAGCACGGGAACGTCCGCCCACGCATGCATTTGAGTCGATACGCGAGCTGGAGGAGTACACGGAACAGACCTTCTCTTCGCTATTGCTCCTGCTGTTGGAGGTGGGCGGAGTGCGTGACCTAAATGCTGATCATGCCGCCTCCCACTTGGGCAAGGCCCAAGGCATCGCCACCCTGCTGCGATCGATACCGCTGGCAGGACGTCAGCAGGCGCCCTGCATTCCGCTCGAGGTGCTGGTGCTGCATGGCGTCAGCCAGGAGCGCATCATTCGCTCTAAGAGCGACGACAAGGGTGTCGAGGACTGTATATTCGATGTGGCCAGTGCCGCCAATACGCACCTAAAGCTCGCCAGGCAGCTGCACGATAAGGTGCCGCCGCAGGTGCGCAAGATCTTCCTCTCCGCCGTGGCCACGGATGCATATTTGGAGCGCCTCCGCCGTGCCAACTTCCTGTGAGTTCCTTTGTTTTATATTGCTTTGTTTTATATTACGACAAACACAGGAGAGAACCAGCTTCTTTTTGTAGTTAAGATGTTATTTTTATCCAGTTATAAGTACGATTTGCCTTTATTGAGACTTCTTTATTTCAAAACCTAGCTATAGTTTGTTGGTTGTTCAGTATTCATGGAGCGGACCCTTCTTT

Search Again