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CRIMIC CR00432

Gene CG30020

CG30020

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:10889293..10889305	47C5	FBti0199372	78911

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGCGTGGTCATCGAGTATATAGACGA	ATGTTGGTGCGGGCTACGGAAACCTTA

Images

Construct Design Sequences

sgRNA sequence	ATTAACTATCGGGTAGGATTGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TCGTGCGATATGTGCCTGTT	CCTACCCGATAGTTAATGCCAA	TCTCTTTAGCTATCGCGCTGA	CGGCCACAGTATCATCCTCC

 

Amplicon 5'

Amplicon 3'

TCGTGCGATATGTGCCTGTTCGAGACGTCCACCGTTAGCCAGTTGCGAACACACTACAAGGAACGGCACACGGTCCAGCCCACGGAGGTGCAGCTGCGTCCCAGTTGGACGGGCGAGCCAACGACAACGAGAGGCGTTGCCGCCAAGCAGCAGACTCCGGCCAAGATATTAAATCACACCGACATCCCGTTGGGCATTAAATGTGAGTCATGAGATCGTTTTAACCAGGGCATTTAAACTGGTATCGACTTTCGCCGGTTTTTTGGACAGTTATGAAATGATTCGCAAACAAAAATTCGTATTAATCTTTTCGTTGGTTGAATGAGTTAATGTTGATATTTATTTATTTATTATTTTCTTATTATTCATTAGGTATTTTACCATACTTTTTGTTGAGCTCTCAACTTACTTCGTGCAATACGTTTTTTTTTATCATATTTTTTGCGGTAATTTTGTGACAAACCATGAACCGAAATTCTACACAACAGTTAAATTAAACGTTTACACTCGAAAATATGTAGATCACAGCAATGAAATTTTAAATTGTAAAATTAGTTGTACATACAAATTTAGCATAACACCGTGTATATCCAGGCTATGTCCTCTTTCTAGTAAATTGATGCTCATAATGATCATAATGGCAATTAGTTTTTTGGCATTAACTATCGGGTAGG

TCTCTTTAGCTATCGCGCTGATGAAACTACAAAAACGTTGAGGCCTTTATTCCATGTAAATAAAAGCAAATCGAATACAAGTGGAGGGATTGTGGACTTTCGGTAAGGACTTCAAAGGAGCACTTTAACTCGTTTTTGATAATTAATTGTTTATAATTACCGTGTTTATCTTTGAGTTTCTCCAGCCTGCACTCTAACCAAATTTTATTTAAGTCCAACAGTTTAAATTTGTATACTCATTTGAATTCCCTGGCTTTCCGTTTTATATGTTTCATGTTGGCATGTTTGTAATGCAATGTCTTCCAGATCCGCCTGGTGTTTTGGAAACGCAAGCGCCATTGCCGCAACCGGTGGTACATCAACCAATGCCTGATCCAATGCAGCAAACGGAGCCGATGGCCACCACATCGACGACCATCACCGAATCGGGCGACATCAACGTAGTGGTGGATGCGACGCCGCTCTTCTATGCGGCAACCTCCAACTCCCTGTTAGTGACGAACCCGGCACCGGTGACAACGGTGGAGGAAACCACCGGTACGGCCAGCAACCATGCATTTGGAATCTTTCCCGAAATCTCAGAGGGCAACCTGCCCGTGAACGAAGCCGTACAGCAGGCACCAGCCAATTCATCTAGCGTTTCGGTGTTTGGCGACATGCAGGACTTTATTGACAACACCGATGTGGCTGCCATATGCACAATTCCCGCGGATGGCATGCCCGTTGTGGATGGTGATGACATTGTGATTGATAATAACAATATCAGTCTGGACTTTGATGCCGAGAATTTGTTCGAGGACTTCGAGGAGGAGGAGGATGATACTGTGGCCG

 

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