CR00434

CRIMIC CR00434

Gene koi

CG44154

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:6813834..6813834	42D4	FBti0200556	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	TATGTTTGTATGCCGTGTGCTGCCG	GTATTCCTCGGCAGTCAAGGCTTTCT

Images

Construct Design Sequences

sgRNA sequence	TGTAGTATGAATTCTCGAACCGG
sgRNA2 sequence

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GGAGACCACACAAAACACGC	GGTACAAGGTCACGGCACA	TTGTACCGGTTCGAGAATTCA	CCTTCTCCCATCAGCCTGTG

Amplicon 5'	Amplicon 3'
GGAGACCACACAAAACACGCTGAATTCCGCCCAGGAAAAGCTAAACCAATCGAACGGTAACCTCAGCTCGGGAAATGTCAGCGATTATCTGGCCTACATCGAGTACAGGGATGCTGGTGAATACTGGAAGTGAGTGAATCATTTCTACACATTTTAAACAGTATACTAATGATGCTATTTTGCAGCAAAACTCCCAAGACGGACTATACCTACTCAGAACTGTCCCCTCACCGACGCCAGTTGGCGCCAGGAATTGTGGCCATGCCCAATATGTCCAGAAAGAGCCTAGAGAACCATAACGATCGAGTCAACTACATGGTCCAGCAGAACCCTGCGCAAGAGGAGTTCATCCGCCGCCGCTACCAGTCCAAGTATACCCAGCAGGTTAACTATGACTCCGCAGATGAACTGGACGCCACATTTGGGCAGCAAAAGCAGAGCTGGTGGCTTATCCGCCTCATCCAACTGGTTGTTAGCAGCATTACCACCGTGTGGAGTCGGGTGACCAATCTCTCGGCCACGGAGACGACTGCCTATCAAAACTACCACGCTAAGCGCCAGCAGAGTCAACAAGTTGGACTTTGGTGGAAAATAGTACAGACCATCGGAGGAGGACTTGCAAGTTTGCTGCGCTACCTGTATGTTTTCATCGGATCGGTGCTGAGTTTGGACACGTGGTTGCTGCGATCCTCGGATGCGGAAAACAAGTCGAAAAAGCGCTTCCTCATATTTCTGCTGATTTTGTTGCCCTTGCTGCTGCTGTCGGGTGAGTAGGATTTAAAGCACCGTCCGATTTTTGATGTAAAGTAAAATATGTTAGAGCGTGTGCCGTGACCTTGTACC	TTGTACCGGTTCGAGAATTCATACTACATGCATATAGATCACCTCCAGTCGTTGTATTTATAAACTCGTCTGGTCGTTGGCAGTCGCGTGACGCCATCGATAATTTGTTAGTAAAATCCCGCGGATCAGATTTAAGTGGCCATGTGATGACATATTTTATTTTCCATTCACCGTCTGCCAAGGTTAAACGCCTAATTTGCTTTGCCTTTGCAGGTCTGTTCTATTACATACACCCCAATGAAACTTTCCCACCCAAATCCCTGTCCGAATACACATTCACCCTACCTGAGTTGCCCAAAATCGATGTGAAAGACTACCTGAACCAGGAGCAGTTCGAATCGCTACGCTCTCAGGCCGCCGAGCATGCGGTCCGCGTTCGCGATTGGGCCGACGACTACGTTTTGTACTTGAGAACCATCGGTCAGAACGTGGTCAACAAGGGTCGTCAGTTATTTCAGGGCGATGATAAAGTCTATTATGAGCGTGTGTAGGGAACATCTGCAATGGGCAGGCTACAGCTACCAGATTTAGTGATCTGGTTGGGTGCAACGCCCCTTGCACTTAGACAAATATGAGAAACTTTCTTTATGAACAATATGAGTACAAAATAAAGCATTTGATCCGTTTTTTGTCCGTCTGCTTGACCCGCAGTTTTGCGCACTTACTTTATAGTTTTTCGTCCCGTCATTTACCCACTTCATAGGCAAACCCACCTCTCATACCCCACCATACTAACCGACACAATTCCCAACAAAATGCAACATCACAGGCTGATGGGAGAAGG

Amplicon 5'

Amplicon 3'

GGAGACCACACAAAACACGCTGAATTCCGCCCAGGAAAAGCTAAACCAATCGAACGGTAACCTCAGCTCGGGAAATGTCAGCGATTATCTGGCCTACATCGAGTACAGGGATGCTGGTGAATACTGGAAGTGAGTGAATCATTTCTACACATTTTAAACAGTATACTAATGATGCTATTTTGCAGCAAAACTCCCAAGACGGACTATACCTACTCAGAACTGTCCCCTCACCGACGCCAGTTGGCGCCAGGAATTGTGGCCATGCCCAATATGTCCAGAAAGAGCCTAGAGAACCATAACGATCGAGTCAACTACATGGTCCAGCAGAACCCTGCGCAAGAGGAGTTCATCCGCCGCCGCTACCAGTCCAAGTATACCCAGCAGGTTAACTATGACTCCGCAGATGAACTGGACGCCACATTTGGGCAGCAAAAGCAGAGCTGGTGGCTTATCCGCCTCATCCAACTGGTTGTTAGCAGCATTACCACCGTGTGGAGTCGGGTGACCAATCTCTCGGCCACGGAGACGACTGCCTATCAAAACTACCACGCTAAGCGCCAGCAGAGTCAACAAGTTGGACTTTGGTGGAAAATAGTACAGACCATCGGAGGAGGACTTGCAAGTTTGCTGCGCTACCTGTATGTTTTCATCGGATCGGTGCTGAGTTTGGACACGTGGTTGCTGCGATCCTCGGATGCGGAAAACAAGTCGAAAAAGCGCTTCCTCATATTTCTGCTGATTTTGTTGCCCTTGCTGCTGCTGTCGGGTGAGTAGGATTTAAAGCACCGTCCGATTTTTGATGTAAAGTAAAATATGTTAGAGCGTGTGCCGTGACCTTGTACC

TTGTACCGGTTCGAGAATTCATACTACATGCATATAGATCACCTCCAGTCGTTGTATTTATAAACTCGTCTGGTCGTTGGCAGTCGCGTGACGCCATCGATAATTTGTTAGTAAAATCCCGCGGATCAGATTTAAGTGGCCATGTGATGACATATTTTATTTTCCATTCACCGTCTGCCAAGGTTAAACGCCTAATTTGCTTTGCCTTTGCAGGTCTGTTCTATTACATACACCCCAATGAAACTTTCCCACCCAAATCCCTGTCCGAATACACATTCACCCTACCTGAGTTGCCCAAAATCGATGTGAAAGACTACCTGAACCAGGAGCAGTTCGAATCGCTACGCTCTCAGGCCGCCGAGCATGCGGTCCGCGTTCGCGATTGGGCCGACGACTACGTTTTGTACTTGAGAACCATCGGTCAGAACGTGGTCAACAAGGGTCGTCAGTTATTTCAGGGCGATGATAAAGTCTATTATGAGCGTGTGTAGGGAACATCTGCAATGGGCAGGCTACAGCTACCAGATTTAGTGATCTGGTTGGGTGCAACGCCCCTTGCACTTAGACAAATATGAGAAACTTTCTTTATGAACAATATGAGTACAAAATAAAGCATTTGATCCGTTTTTTGTCCGTCTGCTTGACCCGCAGTTTTGCGCACTTACTTTATAGTTTTTCGTCCCGTCATTTACCCACTTCATAGGCAAACCCACCTCTCATACCCCACCATACTAACCGACACAATTCCCAACAAAATGCAACATCACAGGCTGATGGGAGAAGG

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