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CRIMIC CR00440

Gene Alg3

CG4084

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:23708455..23708457	59F6	FBti0199374	78913

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCCAGTTGGACTGCTATCGATGAAGGTA	TTTCACGCCGGTTGGTATTTTCGCTC

Images

Construct Design Sequences

sgRNA sequence	GATTCCGTGGATCTGCCCGGGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ACACGAACAAGGAGGATCCG	GATTCCGTGGATCTGCCCG	CGAATCGCAAAGACGGCAG	AAGTACCTCAACGTGCGCTT

 

Amplicon 5'

Amplicon 3'

ACACGAACAAGGAGGATCCGGATGCGGGCCGCAAGTTCCAGGAGGTCTCCGAGGCCTACGAAGTGCTCAGCGACGAGCAGAAGCGCCGTGAGTACGACACCTATGGCCAGACGGCGGAAAACATTGGTCGCCAGGGCGGCGGATTCCCTGGCGGAGGAGCCGGTGGCTTTGGCCCCGAGGGTTTCTCGCAGAGCTGGCAGTTCCGCTCGAGCATCGACCCCGAGGAGCTCTTCCGCAAGATCTTCGGCGAGGGCAACTTTCGAACGAACTCGTTCGACGACTTCGCCGACTCCAAGTTCGGATTCGGCCAGGCCCAGGAGATGGTCATGGACCTGACCTTTGCCCAGGCGGCGCGCGGCGTTAACAAGGATGTCAACGTCAACGTGGTGGACCAGTGCCCCAAGTGCGCCGGCACCAAGTGCGAGCCGGGCACCAAGCCAGGTCGCTGTCAGTACTGCAACGGCACCGGCTTCGAAACGGTGTCCACTGGGCCCTTCGTAATGCGCTCCACTTGCCGCTACTGCCAGGGCACGCGGCAGCACATTAAGTATCCGTGCAGCGAGTGCGAGGGCAAGGGCCGCACGGTCCAGCGCCGCAAGGTCACCGTGCCGGTGCCGGCTGGCATTGAGAACGGGCAAACGGTGCGAATGCAGGTGGGCAGCAAGGAGCTGTTCGTCACGTTCCGCGTGGAGCGGAGCGACTACTTCCGGCGCGAGGGCGCCGATGTGCACACGGACGCGGCCATATCCCTGGCCCAGGCGGTGCTGGGCGGCACTGTGCGTGTCCAGGGCGTATACGAGGATCAGTGGATCAACGTGGAGCCGGGCACCTCGTCGCACCACAAGATCATGCTGCGCGGCAAGGGTCTGAAACGCGTGAATGCCCACGGACACGGCGATCATTACGTGCACGTCAAGATCACGGTGCCATCGGCAAAGAAGCTGGACAAGAAGCGACTGGCTCTGATCGAGGCGTACGCCGAGCTGGAGGAGGACACCCCCGGGCAGATCCACGGAATC

CGAATCGCAAAGACGGCAGTAAGCAAGCAACGTAAATAATCTCCCGATCTACCAATGCAATCTCTAAATGTAACTTGGAGCTTGAATTCGTAGTTGAAATATACGTGCGTTCTATGTGCGAGCTTTATTAAATGATGGCGTCTTTCTCTCCCCTTCCCCATCTCTCGTTGCAGCGCAGGAGCGAGTGAGGAGCCAGGAGCGGGAGCAGCTGCTAAAGCCAGTGCAGCAGCCGCCGGATCAGGAGCCTCCAAGCCAGGACCAGGAGCAGAAAAAAGCGAAGGCAAAGACCAGTGGACGGACAACGAGAAAACCAAAGCGAAAGAAGGCGGCGGATCCGGATCCGGACAAGGCGACGGCGGAGGCGGAGGCTTCATAAGCAAGATCAAGTCCATGTTCAACTGACAGGCGTTGGCAACCCGATGGCCGATTCTGTTTTTTTAGTGTCTAAGTTAACGCACGTTATTGTTGCTAATCTAAGTTCGGGCGGGAACCGGGGGAGTGTATTCCAAATACGACTCGGTTCGAGATGAATAAACCAAATTGACCGTCAGTCGGGCAAGTTCTGCGTTTAAGCGAGGCTAACTTCACTTGTCTTAATCTCACGCTGCTCCGATTTTCAGTTGCCTAAAGTTCGTGCCGCATTTGATGTCGTCCAACTCCCCAAATCTCTGTGTTTAACCTTTGATGGTAACTGTGTTCAAACATTTGAACCATATGTCAAACTACTCTCCTCGATTTTGAAAATACTTCAAACTTTAAAGTAACTGAAATATCAATTTTATAAGAATTCCAACTAAAAAAGAGCATAAACTATATAGCTAGTTGGAAATAGGCTATCCCCTTAGTAAGGTAACCTAAAATATCGTTATAACCACATCATGCGAAGAACAACAGGTGCACGCATATGTTTCCGCAACCGGAATGGGGTCACCTGTGGCCAAAAATAAACCAGGAGCGGCGCAGAAACTCACCGCGCAACAGACTGTAGTTGGTGGTGCCGTTCAGGAAGCCCTCGCACTCCTGCATGTAAGCCACCCAGTCGATCTCTGTGTAGGGCACCCGCTGGATGACCACCACGTTGATGACCAGCTCCGCCAGGAGCACGAAGAGGCCAACTATGGGCAGAGCGGCCGGCTCCAGGAGCAGATACTTGAAGAAGCGCACGTTGAGGTACTT

 

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