CR00442

CRIMIC CR00442

Gene Gabat

CG7433

Information

Donor Location Cytology FBti Stock number
pM37-p03L:19999755..1999978776D8FBti0200557Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
short band no band GCACTAATTTGAAACGGCGGACTGGA ATGAAGTCGTCGTTGTGGAAGTAGCC

Images

Construct Design Sequences

sgRNA sequence AGTTGAAAAGTAAGACACTAGGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
TCGAATGTGTGGTGGACAGG GCATTTTTATAGGCCACCGG AAGTAAGACACTAGGGGGCT GCCCCGCAGAGCAAAATATG
 
Amplicon 5' Amplicon 3'
TCGAATGTGTGGTGGACAGGGCTCCCAATGTTCGCCCATGGAAGGCTCCCTTGAATGAGAGAATACTGAGTTTCGGGGCTCCTGGGGGAATGTTAATCATGCAGGAGTTCTTCTCCTGCTCGGTAAGAGGTGCATTACCACGAAGTTTGTTCTGGTACCTGAAAACGAAATATTTGCAGCACGTTACAGCATTCCAACAGGGTTTAACTTTAATACTTAAAAAGGCTGAAGAATGACTAAAACTAAAGACTTTAGTACTCACCATATGAAGATGCTCTTGTAGGCATTTTCATTCGAGCATGAACCACACATCATGGTTGTGATTTTATTGAGTCCTTTCGGAGCTATGTTCAAGAGAACTGAGTGCAGTTTTTCCGGCCATTCCTTGCCAGGGAAGACACCAAGTGCAGGTCGGTTAATCAATGCTGGAAATAGTCAAGGATATAGTCACATTATTCAACATGGGAGTTATGTGGCTAAGATAAAACAATATCTTAGGGTTCTTCAACTACATATATGTATGTAGTATATGATATAACTTCTTACTTTTCAGGTTTTGCTCATTGTTAAAGACCTTGTAGAGCCTCGGATGGTTGTAACCCAATGGAACTGAGGAAATCTGGGTGTAGACATCTAGTAGAATGTTGCCATCCACGTCGTAGATGTAGTTTCCAATGGACTTCTCGTAGTCAGCGAAGAATTGGATCGTTCCGGTGGCCTATAAAAATGC AAGTAAGACACTAGGGGGCTATCTCTAAGTGGGCATATGGCATATGGTCTGATTTGAGAACGAAAAGTGCAATAAATATACATATTAATGTGCTTGTGGCGAAAGTACAATATAACCCTCACAACCCAGATAAGGCCGAAGAAACTCACCTGAACAGAATCCAGTTGCTTCTTAAGCTCAATCGATTTGGGGCCAGGTATCGATTTGGTTTTCACTACAGGCTCCTTGGGCTCAACGTTGGTGGAGTACCTCTTGGTACTCAGCCTATATAGACCTACAAGGATACCCAATCAGTTCCAGATTCGCTGGAGCTTAAGGATTTTCATTAGCTTACTCTGCTTGGTCAACGTATTTAGCCACAAGCTCCGCATGGTTACCGTCGATTTGAGTGCTCCGGGGTTAAGTGAAGGAATTGCTCAATAGGAGGGCTCGTTGCTGGCCACTAATACTTAGTTAATAAAGAAAACGTATATATTTACAGAATACAAACATAGAGGCTTTTATGTATGCGGGCATATACACCTATCCATATTCTTAAGCACAACCACATTCTCCCAGTTCGGATTTTATCAATTTAACTTTTGGAATCATCATGGTTCTGCGTCAATTACATATTCTGCAGTGAAAGTAAACGAAAGTTGAAAAAAAAACCAAACCATATTTTGCTCTGCGGGGC
 

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