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CRIMIC CR00446

Gene Dsor1

CG15793

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	X:9248013..9248013	8D2	FBti0199375	78914

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AATCCCTTCCTGCCAGTGGCCCAAGTA	GTTTTTGCCCAGCGTCAACGCTCTATTG

Images

Construct Design Sequences

sgRNA sequence	TGAGTAGGAAACCGAAAACGAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TTTCGCTGTTCCTGATCCCC	GGAGTGGAAGGGGAAACAGC	TCCACTCCTCGTTTTCGGTT	CTGGCCATGATCAGGTGTGT

 

Amplicon 5'

Amplicon 3'

TTTCGCTGTTCCTGATCCCCGTCAGCACGAAGATCATGATCAGGACAATGGCCGATCGCATGTTGACCACAATGCACTGTCGTTGGAGCCTCATTTTGGTCAGATAGAGCGGGGAATACGCCGGAAATGTTATTGTTTTTCCCTTTAAGAAAATGAGTGGGGTGGGAGAGTGTGACCGTATACGCGTGGCATGAAAATATCAACCGCGAATGCTCAGGAGAAATTTCTTTAAAAAGCGTATCGAATAGTTTATAGATATGTTTTGGCTCAATTAAATTGGCTAAACAGATAAGTATACCTCTCCCCTCATCGTAACAAATTAGTAATATCTAATCCTAATATCCTTTCCATTTAAGTTGTGACCATTGGCCGCTTTAGTCGTAGGAGTGTATTAACGGTATTTGGTAATCGCGACATCTGGCCATACCGCACCGTCTGCGAAAAAAACCCAAAAGTCGCCGATTTTGGCTGATTATTGCAAAAATAATATTAACTATCCGTTTGCTGTTCGTGTGCTCGGTTCGGGTGTGTTAAAGTTAAGCGCAACGCGAGGAATTGGTGCAAATTTACCAGCGGCTGGACACACAAAACGAAACATTCGCACTTGGCCTCCCTTCGCACGCCCCCTCGCCGACGCCCCTCGCCCCTCACACCCTTCAGCCGTTCGTCGGACGCCGCTGTTCGTAGTGCGTTTTGTTGTTGTTTCCACTGGAACACTCTCTATTTGTATTGTTTCATTTATTAGTTTTTTTTTGTTGTTGTTATTGTTGTGTGCTTTAACTTATCGGTAACGGATAACGGTATCGAGAGATATATATTATATATAGTAAGATGTCGAAGAACAAGCTGAATCTGGTGCTGCCGCCGGTAAATACGGAGGCAACTGTTGCCGCCGCCACAGTGGCGCCGACGCCGCCATTCAAAACCCCCTCGGGCACAGAGTAAATATTATACATAATATCCTTAATAACTGACTGGTTTTTAGGCCACCCATTCCAATAACACCGCATCCCCCCTCCCTCGCTGTTTCCCCTTCCACTCC

TCCACTCCTCGTTTTCGGTTTCCTACTCAGTGGTAAGATAATCCTGTAGTTTTGCGAGTTAGGTGGGTAATTAATATCCATCAATTGATCCAAGGAAATCCCATCCAGCTGGAAAGAAAGAGACAGTCGGAGTGAAAGAGCAGGTACTCTCTCTTTGTTTGTTGGTGTGGGTGGTGCTCCTTTCGTGTGACGCACAGGTACACAGCGAGAAAAAACTTACTGAGTAAATACAGTAAACTGTTTTAGTGCTTTTTGGATTAATTAACGGGATGACAAAGTCAGTTTCGAAGATAAATAGAAATTATAATTTAATAGTCCTACCTTTATCGTATATTTTTTTGTGAGCACCAAAATGTAGGCAACATAACATAACACAATACATATGTAATTTGATGTGTATGTTTGTGTAGTTTTCTTATTATTAGGAGAAACTTCGGCCCGCCCCTCATCCCATCCCCCACTTTCAAATCCCATCCATTGCTTGCACTTTTTACATTTTCGTTTTGGATCGCAAAAATTCATTAAGAGGTTAAATTAAAGCACACACAGTTTGCTGGGGAAGCCCAAGACGAGCATCGATGCGCTGACAGAGACGCTGGAGGGTCTGGACATGGGCGACACGGAACGCAAGCGGATCAAGATGTTCCTCAGCCAGAAGGAGAAGATCGGCGAGCTGTCCGACGAGGATCTGGAGAAGCTGGGCGAGCTCGGATCGGGCAATGGCGGCGTGGTGATGAAGGTCCGGCACACGCACACACACCTGATCATGGCCAG

 

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