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CRIMIC CR00448

Gene DAT

CG8380

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:16562699..16562699	53C7	FBti0199586	79260

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	short band

Images

Construct Design Sequences

sgRNA sequence	TGCTGCCTGCAACTCGGAAGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ATCCATGACGCTCGTCTCTG	CTGCCTGCAACTCGGAAGT	GGCAGCAGGAAAATGGGAAA	TACACATCATTGGGCTCGCC

 

Amplicon 5'

Amplicon 3'

ATCCATGACGCTCGTCTCTGTTGTTGAAGCCATAATTTCCGTTGCTTCAAGTGCATTTCCACCATTTTCCAGCAGCTCGTCGGAGACACTGTGGAAATATGTGCCTCTAATTCGATTCCCATCCCGTAATTCGGTCAAAACTTGTCCAATTTTAAACGGCCAAGTGTTGGTTATTGCTTGACTAGCAAATTGATATTCTATTGAATTATTGAAAGTTATGCCTACTTAAATGCTTGTGAATAACTTTTTACATGTATTTGTGTAGGACTATTCTGGTTTAAGCTGAGGTGCAAAGTCTCACCATTAAGTCCTTCAGTTCCAAATAGAAATCTAAACTTTTAGACAAATTTAAGAATAAAGAATATAATTTATTGAAATACATAGTTAACCCAAGTACAATCAAGTGCGTATGCGCGATCTTAGTTTGAAAAAAAGCCAGGCAGCTCCTTGAGTTTTAGCCCTAATCCAGAACAATGTGGATGGCACAGCCTGTTGCTGCCACCAAAGCTTCCACTTCCGAGTTGCAGGCAG

GGCAGCAGGAAAATGGGAAACAGAATAAATAATACCGACTAAGCTGTGGTAATAATCGCAACCTGAGCGAGTTGCTGCCCCTGGCAACGGACATAAAATGGAACTTAAGCTGAAGATGGAGCTGGAACCACTGATTGAACGCAATGAAGCGTATAAATATAACAATTTATTATTATGATTTTAAAGCAGCAGCAGCAGCAGCGACCGGGGGTTAGCAGGAGGAAAAAAAGAAAACTCATAAAATAGTAATAACTATATCGAAGGCCCTGTGTGCCACATCCATTCACGACGCATCAGCGGGAAAGTGCCGCAATCAGAGGGAAAATGGGGAGGTGGAGACATGGAGACATGTTTCGCACATGAATTTCCCACAATGCGATGGTGGAAAGTGCTCCAGCAACAAAAGCCTAGAGAACTCCACATAAGCAGGCTGACATATTGCCAAGAAAATGGGGTGAAAAAGTGTGACAGAGGGTAAGCAGGCCAAATGCGATTATGACTGCCAGATTTAGTTGTCATACAGAAGCACTTAACTTTTGGAATTGTTTTGAATGATGGCGTCGGCGATGAGGATCAGGAAGCGAAGTAAGTGTTCGACTGTGTGTCCTTTGCCGCTCTTTTAGCGGGGAAAAGCCCTGCAATTTGACAAAGGAACCATTCCCGCACCACCCCACCGACAGCACTTCGCACCACTTTGACAAAGTGTTAACAAGGCCGAAAGGGAAATTCAGAAAGCGGAAAGAGGAAAGCGACGGGTTCCGTGGCGAGCCCAATGATGTGTA

 

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