CR00449
CRIMIC CR00449
CG10302
Information
PCR Verification
PCR check relative to sgRNA sequence
HA-L |
HA-R |
Forward |
Reverse |
short band |
OK |
TTGCAGGCTCTTGCAGATGGACAAGAT |
CCGTGTGCAACGATACGTTTGCAAATGA |
Images
Construct Design Sequences
sgRNA sequence |
ACTATGTGCTCATCCAATTTTGG |
sgRNA2 sequence |
|
Amplicon PCR 5' Left |
Amplicon PCR 5' Right |
Amplicon PCR 3' Left |
Amplicon PCR 3' Right |
GCGCGCATGAATTCCAGAAT |
TGGCCTTAGTCGAGCGTTTT |
GCACATAGTAGGCGCTCCAA |
ATCACTATTAGGCGCTGGGC |
Amplicon 5' |
Amplicon 3' |
GCGCGCATGAATTCCAGAATCCTAGTGGCTCCTTCAATGTCTCCCTGCTGACAATAGCGAGCAATCAATCGCTGATAGGTCACCCTATTGGGCTCGATGCCCTTCGCCTCGATCTCTGCCAGAAAATCGGTGGGAGCAAACCCATGCTCGTTCTCCAGGTAAACGCGAAGCAGGGCATTGTAGTGCGAGATGTCCATGGGTACATTCAGCGCATTCAGCGTCTTCCAGATCTCCTGCACCAAGGCAGTTCTGACCTCGGGAAGCTCCTCCGGTACCAGGTTGCCGCAGCAGCGAATCACCAGCAGCGATTGCGAGCTGGTGGCCGTCCTGTGTGAGCGGATCTCATCGAGCACCTCCTCCAGATCGCGTCTGGAGATGCGCCCAATGCGACGAACATCCTGGTCCAGCCGGCGAATCTGTTTATCCAAACTCAACTCAGCTTTGGCCGCCGCATTCGTGGCAAAACCACTAGGAAGATAATTAAAAATAAACTTAATTAATATATAAAGATATATGAATTAACAAAATCCTAACGTCCTTATAGATTATTTTCCGGCTGACCTTAATGAGAAACTGAGTTGTTATAAGATAATCGTGTTGCTTTTTGAATAAAAATAAGATATTTAGCTTCTTTCTACATATATAACTAGACTTTCTACAAGAAAAACGCTCGACTAAGGCCA |
GCACATAGTAGGCGCTCCAAATCCGGATGAGGTTATGTAAATATGTGAGACATCAGTCGAATGCAACTGCGATGATCCCAAGTGGCGGCACGTGCGCCTCTTTTCGGGATTCAATCGCTGGAAAGTGGACGATTCCCCACTAATCACGTAAATACATGAGCACATTGCGTAATGCCGGCGATTTGGGGCCAGATTTTACTCACTTTTGGAACTGGCCGCACATGCAGGGCGCATTGTTCAGCAGATTGCTCGTCTCGTTCTCGCGAACCGAGTTCACCAGCACATTGCGTGTGAAGCCGGCGAAGTAGCGCAGCAGTTTGCCCGTCCTCAGGATGGATGCCATGGCGGAATCGTTCGGTTTTAATTAATAAATGAGCTTATTATTAAAATGACGTTCGCACGAGTGCGGCTGAAACCGATGTCTCTTCGCGCCTATCGATGTTTACCTGCGACAATCGATTGTTTTCGGTGGGACCAAAACGTTCAAATCAAATGTATCAGCTTAGCGGTGACACAAACGAGAGGATATTTGAACAGTGCCTAAGCCTGTCAGCTTAACCGATTTAATTTGCTTTTTTTCGGATCGGAAACTCTAATCGTACCCCGTATATACTTGAACTAAAATGTCTCTGAATCTGCAGTACGAGGACATTGGCAAGGAATTTGTCCAGCAGTACTACGCCATATTCGATGACCCGGCGAATCGGGAGAACGTGATTAATTTCTATAACGCTACCGACTCTTTCATGACCTTTGAAGGCAACCAAATACAGGGAGCACCCAAGATTCTGGAAAAAGTTCAGAGTCTGAGCTTTCAGAAGATTGCCAGAGTGATAACCACAGTGGATTCGCAGCCAACTTCCGATGGCGGAGTTCTGATCATCGTCCTTGGAAGACTAAAATGCGATGACGATCCCCCACATGCATTCTCGCAGATCTTTTTGCTGAAGCCCAACGGAGGATCCCTCTTCGTGGCTCACGACATCTTCCGTCTGAACATCCACAACTCTGCCTAGGAGCACTCCAGACCCATATGTACACCACACATAATCGACATCCAAAGACGCCCAGCGCCTAATAGTGAT |
Search Again