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CRIMIC CR00449

Gene bsf

CG10302

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:18454132..18454144	36F5	FBti0199587	79261

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	OK	TTGCAGGCTCTTGCAGATGGACAAGAT	CCGTGTGCAACGATACGTTTGCAAATGA

Images

Construct Design Sequences

sgRNA sequence	ACTATGTGCTCATCCAATTTTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCGCGCATGAATTCCAGAAT	TGGCCTTAGTCGAGCGTTTT	GCACATAGTAGGCGCTCCAA	ATCACTATTAGGCGCTGGGC

 

Amplicon 5'

Amplicon 3'

GCGCGCATGAATTCCAGAATCCTAGTGGCTCCTTCAATGTCTCCCTGCTGACAATAGCGAGCAATCAATCGCTGATAGGTCACCCTATTGGGCTCGATGCCCTTCGCCTCGATCTCTGCCAGAAAATCGGTGGGAGCAAACCCATGCTCGTTCTCCAGGTAAACGCGAAGCAGGGCATTGTAGTGCGAGATGTCCATGGGTACATTCAGCGCATTCAGCGTCTTCCAGATCTCCTGCACCAAGGCAGTTCTGACCTCGGGAAGCTCCTCCGGTACCAGGTTGCCGCAGCAGCGAATCACCAGCAGCGATTGCGAGCTGGTGGCCGTCCTGTGTGAGCGGATCTCATCGAGCACCTCCTCCAGATCGCGTCTGGAGATGCGCCCAATGCGACGAACATCCTGGTCCAGCCGGCGAATCTGTTTATCCAAACTCAACTCAGCTTTGGCCGCCGCATTCGTGGCAAAACCACTAGGAAGATAATTAAAAATAAACTTAATTAATATATAAAGATATATGAATTAACAAAATCCTAACGTCCTTATAGATTATTTTCCGGCTGACCTTAATGAGAAACTGAGTTGTTATAAGATAATCGTGTTGCTTTTTGAATAAAAATAAGATATTTAGCTTCTTTCTACATATATAACTAGACTTTCTACAAGAAAAACGCTCGACTAAGGCCA

GCACATAGTAGGCGCTCCAAATCCGGATGAGGTTATGTAAATATGTGAGACATCAGTCGAATGCAACTGCGATGATCCCAAGTGGCGGCACGTGCGCCTCTTTTCGGGATTCAATCGCTGGAAAGTGGACGATTCCCCACTAATCACGTAAATACATGAGCACATTGCGTAATGCCGGCGATTTGGGGCCAGATTTTACTCACTTTTGGAACTGGCCGCACATGCAGGGCGCATTGTTCAGCAGATTGCTCGTCTCGTTCTCGCGAACCGAGTTCACCAGCACATTGCGTGTGAAGCCGGCGAAGTAGCGCAGCAGTTTGCCCGTCCTCAGGATGGATGCCATGGCGGAATCGTTCGGTTTTAATTAATAAATGAGCTTATTATTAAAATGACGTTCGCACGAGTGCGGCTGAAACCGATGTCTCTTCGCGCCTATCGATGTTTACCTGCGACAATCGATTGTTTTCGGTGGGACCAAAACGTTCAAATCAAATGTATCAGCTTAGCGGTGACACAAACGAGAGGATATTTGAACAGTGCCTAAGCCTGTCAGCTTAACCGATTTAATTTGCTTTTTTTCGGATCGGAAACTCTAATCGTACCCCGTATATACTTGAACTAAAATGTCTCTGAATCTGCAGTACGAGGACATTGGCAAGGAATTTGTCCAGCAGTACTACGCCATATTCGATGACCCGGCGAATCGGGAGAACGTGATTAATTTCTATAACGCTACCGACTCTTTCATGACCTTTGAAGGCAACCAAATACAGGGAGCACCCAAGATTCTGGAAAAAGTTCAGAGTCTGAGCTTTCAGAAGATTGCCAGAGTGATAACCACAGTGGATTCGCAGCCAACTTCCGATGGCGGAGTTCTGATCATCGTCCTTGGAAGACTAAAATGCGATGACGATCCCCCACATGCATTCTCGCAGATCTTTTTGCTGAAGCCCAACGGAGGATCCCTCTTCGTGGCTCACGACATCTTCCGTCTGAACATCCACAACTCTGCCTAGGAGCACTCCAGACCCATATGTACACCACACATAATCGACATCCAAAGACGCCCAGCGCCTAATAGTGAT

 

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