CR00450

CRIMIC CR00450

Gene CG7430

CG7430

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3L:17847269..17847273	75A4	FBti0200558	80582

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	OK	TTGAACTTGGTGAGTGTGTGGTGCAG	ATCTCCTCTTTCTCGCCAGACTTGG

Images

Construct Design Sequences

sgRNA sequence	TTGCATAACTTGAGGGGCGATGG
sgRNA2 sequence

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CAGATTTCGGGGGCAAGTCT	TGGGGAATCGGTTATGTCGC	GCCATCGCCCCTCAAGTTAT	CACCTCGTTGGGATTGACGA

Amplicon 5'	Amplicon 3'
CAGATTTCGGGGGCAAGTCTCGCCATCTTGTTGTGTCCGCGAACCAGTGTGACCGCATTGCGACAGCTAGCGAAATACTGAGGCTTCAACGAAAACGCGAGATGAACGCGACGTGAAAATACCAAAAGTGTTGTGGGAAATATCCCAGATGGGGAATACCACACAGGGTTGTACTGTAGGGCAAACTATCGAATATTCATCGTATAATACATTTTTAATTACCTATAGATATAATAAAGTTAATATTACTTTGGAACTCTTATTATATATGTATCGTATGTACGTTGAGTTATATATTTTTAAAACAGTTATTTTACAGTTTATTTATTACAGTTTATTTTATACGTACAAAAACATTAATTAATGGGTGCCAAAACTATAAAAGCCGGCTTACACGTATAGTTGGCTTGTTTTTAGGTAGCTTTTGCTACAACAAATGACCCATAAAGTGAATATTGTATAAACTAGCAATTGAGTGACGGACATATCGTATTTTGAACGATCATCAAGCTAATATTTTTATACACATCTTTTTCATACGAAAATGTTTTAGAATTTCAAATTCCAACAGAGTGGAAAATCACCAAAACAATCGATAGCATCGCGGCCATCATATGACCACTATCGAATGCACATCGCCAGCCCTGCGACCGCTCCGAGCCCCACAACACGAAAAACAGTCGGAAAAACACACATCAAACTAGTGTTGTGTTTGTAGTTAAATAACGATTATCCTCATAACTCGCCCGCACCAGCCATGCAATTCACGCTCCGTCATGTTGTTTCGGCAGTAGCCAAGGTAAGGATCGGGCGACATAACCGATTCCCCA	GCCATCGCCCCTCAAGTTATGCAATCTAACCTCACCGAGGGGGTCGAGAAATGGGCAGGAAAAGGCTGGAAAATGCACTTGGATCACCTGCAGTGCTCATATGATCCCGGTGGGTGATTCACTGGCGTTTGGGGATTTGTTTGGCCCCGTTTTCAAGGTCGCTAATTAGGCCAATTATCAGCTGATAGTCGGCCGAAGATTAGTGCGAAATGCGTGCGACTGGCGTGATAAATGATAAGGTGCCACAACATGTAGTAATCTGCCGTCTTTACAGACTCCTCTGCGCACCAATGCCGCCATTCTGGGTGCCCTCAACGCCCGCTGCTACTCCAGCACCCACGAGGCGGACATCGTGGTGATTGGCTCCGGACCCGGTGGCTATGTCGCCGCCATCAAGGCCGCCCAGATGGGCATGAAGACGGTCAGCGTGGAGAAGGAGGCAACTCTGGGCGGCACCTGCCTGAATGTTGGCTGCATTCCCTCAAAGGCGCTGCTGAACAACTCCCACTACTACCACATGGCCCACTCCGGTGATCTGGAGAAACGTGGTATTAGCTGCGGCAGCGTTAGCCTGGATCTGGAGAAGCTTATGGGTCAAAAGTCCAACGCCGTGAAGGCTTTGACCGGTGGCATTGCCATGCTTTTCAAGAAGAACAAGGTCACACAGCTGACGGGCTTCGGCACCATCGTCAATCCCAACGAGGTG

Amplicon 5'

Amplicon 3'

CAGATTTCGGGGGCAAGTCTCGCCATCTTGTTGTGTCCGCGAACCAGTGTGACCGCATTGCGACAGCTAGCGAAATACTGAGGCTTCAACGAAAACGCGAGATGAACGCGACGTGAAAATACCAAAAGTGTTGTGGGAAATATCCCAGATGGGGAATACCACACAGGGTTGTACTGTAGGGCAAACTATCGAATATTCATCGTATAATACATTTTTAATTACCTATAGATATAATAAAGTTAATATTACTTTGGAACTCTTATTATATATGTATCGTATGTACGTTGAGTTATATATTTTTAAAACAGTTATTTTACAGTTTATTTATTACAGTTTATTTTATACGTACAAAAACATTAATTAATGGGTGCCAAAACTATAAAAGCCGGCTTACACGTATAGTTGGCTTGTTTTTAGGTAGCTTTTGCTACAACAAATGACCCATAAAGTGAATATTGTATAAACTAGCAATTGAGTGACGGACATATCGTATTTTGAACGATCATCAAGCTAATATTTTTATACACATCTTTTTCATACGAAAATGTTTTAGAATTTCAAATTCCAACAGAGTGGAAAATCACCAAAACAATCGATAGCATCGCGGCCATCATATGACCACTATCGAATGCACATCGCCAGCCCTGCGACCGCTCCGAGCCCCACAACACGAAAAACAGTCGGAAAAACACACATCAAACTAGTGTTGTGTTTGTAGTTAAATAACGATTATCCTCATAACTCGCCCGCACCAGCCATGCAATTCACGCTCCGTCATGTTGTTTCGGCAGTAGCCAAGGTAAGGATCGGGCGACATAACCGATTCCCCA

GCCATCGCCCCTCAAGTTATGCAATCTAACCTCACCGAGGGGGTCGAGAAATGGGCAGGAAAAGGCTGGAAAATGCACTTGGATCACCTGCAGTGCTCATATGATCCCGGTGGGTGATTCACTGGCGTTTGGGGATTTGTTTGGCCCCGTTTTCAAGGTCGCTAATTAGGCCAATTATCAGCTGATAGTCGGCCGAAGATTAGTGCGAAATGCGTGCGACTGGCGTGATAAATGATAAGGTGCCACAACATGTAGTAATCTGCCGTCTTTACAGACTCCTCTGCGCACCAATGCCGCCATTCTGGGTGCCCTCAACGCCCGCTGCTACTCCAGCACCCACGAGGCGGACATCGTGGTGATTGGCTCCGGACCCGGTGGCTATGTCGCCGCCATCAAGGCCGCCCAGATGGGCATGAAGACGGTCAGCGTGGAGAAGGAGGCAACTCTGGGCGGCACCTGCCTGAATGTTGGCTGCATTCCCTCAAAGGCGCTGCTGAACAACTCCCACTACTACCACATGGCCCACTCCGGTGATCTGGAGAAACGTGGTATTAGCTGCGGCAGCGTTAGCCTGGATCTGGAGAAGCTTATGGGTCAAAAGTCCAACGCCGTGAAGGCTTTGACCGGTGGCATTGCCATGCTTTTCAAGAAGAACAAGGTCACACAGCTGACGGGCTTCGGCACCATCGTCAATCCCAACGAGGTG

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