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CRIMIC CR00451

Gene Sox21a

CG7345

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3L:14105355..14105377	70D2	FBti0200559	83174

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	no band	TCACCGAAGGACAGAAGCGTCCATT	TTGGCGGTGACTCCTTACAGATTGCA

Images

Construct Design Sequences

sgRNA sequence	GCTTAAACTAATGGCCATTCGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GGTGCTCATCCTAGACCACG	GGGGTTTTCGAGCGAGCATA	CCTTCTGACGAGCCCTGATC	GGCAACACGTCGCATGAAAT

 

Amplicon 5'

Amplicon 3'

GGTGCTCATCCTAGACCACGTGTTCCGCGGATGAGGAGGTGCCGGCGCCGAAGCGCAATGGAGTCGGTCAAATGATGTTTGGCGGACTGGCCGCGCTGGCCACTACCGCATTTGGTGAGGACTTCTCTGACTTGACGCATCCGTAGGATGAGATGCCGGTACCGCTGCTGCTGGGCGGGGAGGGTGGAGTTTCCGGACTTATCAGCGGCAGATATCGCCACGGGTGGTAGATGGAGCCGGCGTACATCGCCTGGAGGCGCGCCTGTAGGTCCAGGGCCAACGTGGGATCCAGTGGATAGTGGGCGAGGCCGGGAAAGGCATTCGGCAGGCGGGGCAGCGATAACTCCAGTGGCGAACCAAAACCGTACTTGGCGCTCAAGGTGGCGGCGGCCAGCTGGACATGATGTGGATGAGGATGCGGGTGGTGTGGATGCGATTGATATGGTGACTGGTGCGGATGTCCGTGGAGATGTGGCAGCGACTGACCCAAGGGCGACAACTGGGGCTGCATATCCTTGGGGGAGCCAACTGATCCTGGGCCACTGGGACCGGAGTTACCCGCACCACCAGCGTTGACACCACCGTTGGCACCACCTCCACCGCCGCCACCACCACCACCTGGCACCGGTGACTTGTTGAGCGTCTTGGGCTTGCGACGTGGTCGATACTTGTAGTCGGGATGTTCTTTCATGTGCAGAGCCCTGTAAATTAAATAAATGTTGAAACACTGATCAAAAGATCGAACGATTTAAGCTTTGATGATATCATAAAGTCAGCCATCTCTGGTCGTAAACCCCAAGAGAAGCGGAACCCTTCCATAAACCCCACGCCAGGCAGATACAAAATGCACAAGTTTATATTGTGTATTTAGCGAAGAACCAGCACTCCGATTCCAACTTCGATTCTCTTTCCGATTTCCCAGCTGCAACAATGCGCAATTGTTGACATTTGTTTGACATAACGATAAAACCTGATAAAAGGTGTGTTTGGGTCGCCTCCTCTTGTTTTACTTTCGCTTTTCTTTATTTTGTTTTCCTTTCTTTGGTCAGTTATGGCCTCCTTATCGCGGGTCCCGAAAAAAAATATTGTTTTCCTTATCATCGCGCATTGGCAAATATTTGATCAGAATTTTTGGTTCTTTTCATTCGATATGCTCGCTCGAAAACCCC

CCTTCTGACGAGCCCTGATCATTTATAAATGTCATAAAATGTAAGTAAAATTTAACATTTCCATTTTGGCAGGCCAATCCAATCAATAAAAGGATTATGACAGTGCAAACACAGAGGTTCGTCATCATAAAAGACCGCACTATCTAGGAAGGCATAGAAAAAACGAACTTGTGAGATACGGCATTTCTGGGAATTCCTGTGGTTTAAAAGTGCACTGCAAATTTTGATTCCATTTATACCAATACAACTTATTTAAAAGCTAGTTTTATAAAAAATAAAATAATGTGAGATATCACTTTTGTACTTATTTGTAAAATGCTCGCTTTTGAGTATATTTCTTGTACAAAGAACTCCATTCTAGCGGTATTTTCAGTTAATCCCGATAATTACGTTTATTAAAACTAAGCTCAAACTGAAATTACTCAAACCCTTTCGAAAGGGTACACTGAATGGGTTAAGCACACAAAAAGAAGCAAAATGCAAAATGAATGGAGCATTTCATGCGACGTGTTGCC

 

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