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CRIMIC CR00454

Gene Dhc93AB

CG3723

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3R:21025565..21025584	93B5	FBti0200560	80583

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	no band	CCGAGGAGTTCAACATGGTCGAGATT	ACACGCTTATCGCAATCTGAGAGCTC

Images

Construct Design Sequences

sgRNA sequence	TCTAGCCCAGAGCCAACTAAAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TATGCTAGTGCGCCTAAGGC	GGCTAGACCTGTGGCTATCT	GCGGGTGTCGATGCTAATCT	AGATGTGCCTCCAGTGTGAC

 

Amplicon 5'

Amplicon 3'

TATGCTAGTGCGCCTAAGGCAAGTCTACTTAGGAGGTGTTTTATTATGAAAACGGTGCACTCTAATAAATAAATCGAAAGTCAAACTTGGAGTCTACGATAAAACTTGGTACATTGGTCTAGCTTATGACTATTATTGTTCTACGTTGTGAACTAGAAAAGAACTACGAAATCGTTTGAACTAAATTAAGTTAGATAACTAAGTTAAGTTAAGCATTAAGCAGTTAGCCATTTAGGTTTGCAAGAGCAGCGCTACGCCGGCCAGGATCCATTTGCCTGGCTTGTCTTTGGTCTTCAGATTCAAGTTGGACACATAGGTGGTGGGGCCTCGGGTGCGGGTCTTGTAGACCGGGCACTCGTACATGTTCCTCAGATCTTGCTTATCTTGGGTGATTGCCTGCGGTACATAGACGAGGTTAAATTGAATCGGATTCGCATTCAGTGTAGTAGAATAATGGAGTGGAATCTAGTTTTCGAATTTATGCCAACATGCGCCAGCAATGAGACGCCACAGATCCGCCCTCGATTCGCCGATTTGCGGATTTTAGCATTTGCCCGATTTGCATTTCCAACTCACCCGAATGTTGATCACCGGCATGGATGGGTAGAGCTCCTTGAGGCGCGACTCCATGATGATACCCTGCTGTATGTCCCACCTGGCGCCCTCCATGAAGATCCCATGCACGCAGCAGCCGTCGCGCGGTGCCGTCCTGCAATTTACAAGATCATCTCAGATGCCGCGACTATAATCGTAGCTATAGATAGCCACAGGTCTAGCC

GCGGGTGTCGATGCTAATCTCCATGCAGAGATGCATATTAATTCCGACGACGGCGCGACGGATCTCCATTGTTCTCCCACCAGCGTCGCCATGTGAAACGTGGCTGGCGGGGCGCCACTTAATAAGCAATCATCTCATCAGCCGTTCAGCCCATCAGCTCATCAGCCCAATCAGTCGTTGGGTAAATAGACAATTGTAATTTGCGGTCTATTAATAAGTCTCACATATTTATATACCAGTGCTGGTTTAAAATTAGCTCTTATGAAATAGCACTGTGCCTATTTTTAGACACACTCGCTGGCCATTGGCCGCAGCTGGATTTTTTCCAGCTTGGAGGCATGATTACATGTCACAACATTACATTCATTATGCGATTGGGATTGAGCTCTGAGCAAATAAGGTCGAGCGGACCATTAGATTATGTTTGGGGAATAAAATTTTTGAGCTAATTCATAGATTTAGTGATTTCGTTTCACACTAAAATGTTTAGGGTTTAAACAAACAGAAACAACTCAAAGTTGGGTCACCTTTTAAGAAATATAAAGATTTTTTCTGGGCCCTAGAATTCCAAAGATTTATCTCTTTGATAAAATGGCAATGTTCGTGCTTTTCTTAGAATCTGCGACTATTGCGAATTTGAGATTGCTCATGATAGTGTAAACATTCCCGGGAAGGGGTCACCTCAAGAGTCAATTGAGAATTGGCGTGGCAATGGCAGCGGCACTCACGTAAATTCCTCCTTTTGTTTTTTGGTCACGTCACACTGGAGGCACATCT

 

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