• About
• Search
• MiMIC
  • Technique
  • Constructs
  • RMCE Lines
• Transposons
  • GDP
  • Others
• CRIMIC
  • Technique
  • Constructs
• Order
• Downloads

CRIMIC CR00456

Gene AstCC

CG14920

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:11084584..11084586	32D2	FBti0200561	80584

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
long band	OK	GGCTGGCATGCAAAACACTTAAGTGG	CACGCCCACAATTTGCACCCGAATAT

Images

Construct Design Sequences

sgRNA sequence	TATGCCAGGTGCACACTATTTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ATCCGGATGGTTGGATACGC	GTTGGTGTCTAACGAGGGCA	CCAAATAGTGTGCACCTGGC	TGCAGGCAGGACATGGATTT

 

Amplicon 5'

Amplicon 3'

ATCCGGATGGTTGGATACGCCGGCTACCAGCTGGGGAACCCGAATGGGTGGACGTTCCAGATCGATCTGCGCCCGCTCTTCTCTGAAAGCTCTTTGGCATTAACTGAAATTTTCTTACACATCGCGCTGCTAATTTTATGTCTTATATTGCAATTTAGGTTTTATGTTCCAACTTACTGTTTCGCCAGTGGCGCGATTGATCTGGTTCAAATCTTTGAAATCATAATCCAACAATGTTCCCGCATCGTCGTCACTGTACATGCCTAAAAGCAAACCACGTTTCTTAGATCTAATCCTTGTATGGATGTAGTAACAATCACATACCATCCTCCCTAGCTTGATGATGCTCCATTCCGTTGTGCGAATCGCCGAATTTGCCACTTAAAATGTTTCTATCTCCGAAGAATTCGCCAATTCTTTGTTCACTTCGTTCCTGTTCGAGGGCATGATGCAAAGCAACCATAAGACGATCCAAAAGTAATGCTGCACGCTTGGGAACCACCATCTAAAAGTGTATAAATGAGAAAATTAGGTATTTGGAAAATGCGTCTTGCTTATAATTGATAAGTGAGCTTTTCTTAGAAACTTCTGTCTGTACTACTGTAATTTTCAATTAGTTAAATGTTTTCTTTTTTCGAGGTCTTTAAACCCACTTCCCACGCATCCCAAACATGAGCCCACGGAATATTTAATTTCTGGATAAAAGTGCTTTCGCCACAGTCTGCCCTCGTTAGACACCAAC

CCAAATAGTGTGCACCTGGCATAATCAAAGTGATGTGACGTCAGCTCGGCTTTCAAGGGGAAATATAGCTAGGGGTCATAGTCGAAAAGCAATCAAATGAAAAATATACAATTACAAATTGTGTGTAAGTTGATAGGGATTCAAAACAGGCAGTGACCAACGCGACCCACGCGAAGATTCCTTCTTAATTTTTTTCGCTTTATAATTTTCGGAGATTTTGCTTACCGGATACTCGTCGTAGCCCTCGTCGGCTGGGGCACTAAAAATCTCCTCCGGCTGACTGGGGGCCGACCTGGATCCACCCGCTCGCTTGTCATCCGCCGGTGTGTTGGCCTCCGGCAATGTCACCTCTCCGGCTGCACTGCCGCCGATGGTGAGCAGCTTGTCGGGGGATCGTTTGTCGGCGCCCGGATACACCATCAATTGCCGGCACATGTGCAGCTCAAAGTTCTGGATGAGGACGAGCAGGATAATCAACATGGCTGGTAGGTGATAGGTTCTGCACAACGGAGTGCCCTCCTTGCCGGCAAGACTTGTGCAACTTCTCCGCCTCCTGGCAGTTTGGCGACCAGGTGGTTGATGCATTCTTGTGTCTACGGGCGGGAAACTAATTAATGTCAAGCCTGTCCTGCGTTGAACACTTTTGAAAAATCAACTGAAGCGTTGGCTTTATGCGTTCGACGCTTTTATAGCAGCCAATTCCTTGGCAAATCACTAGTTTTTTTTTTTCTTTTGATTTACTTTTTTCGTCCTGCTACTGCTTTTTTACAGTAATGAAGCATTATAGACGCTTGTTAATCGGGATGGAGCTGGTCCAACAGTGAAATGCTTTTTTCATACTTTGATTTACGATTGCGGCACCCACTCGGCGTCCTTGTTGTTGCTCCTTGGTTGCGGCTCTTGCTGTAATTACTGAACATCAAAATCCATGTCCTGCCTGCA

 

---

Search Again