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CRIMIC CR00458

Gene CG14414

CG14414

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	X:14581524..14581524	12F1	FBti0199377	78916

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGCTACCGTGTGGTGAATAGCTGA	GGGAGTTAAGAAGAAGCTAGCTCAGTTC

Images

Construct Design Sequences

sgRNA sequence	GTAATAAATATCATTCAAGGCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TGCCCCCTATCGGTTTCAAC	GCGGTGCAGCCACATATTTA	CTGCACCGCCTTGAATGATA	CAAAGTCGAGTGTGGGTCGA

 

Amplicon 5'

Amplicon 3'

TGCCCCCTATCGGTTTCAACTCCTCAAACTGATGCAAAAATGCGGCGCCATCGAGAAGTTCGACATGCTGTTCCACAAGGGCGGTCCCATGGTGGGACAATCCAGGGGCTACGCCTTCGTCACCTTTGCGCAGGTGGGTGCAGTGCCTCATTGGCGGCAGGCGGTGCAGCGGCTTTAATCAAGAAAGGATCAAGGATTATTGTGCTTTTTAACTTGGCTATTTTGGTATTTAATCCTCAGTATTCTCAAGCTCATTTATGCAGTGCGGCGAACTCTGTATCTATACTCGCAGATGCACTAGCCTAAAAACGCAGCTATGTTATTTTTATATCTGTCACCCATACAGCAAAAGGGTACACGACTTGCAGAAAAGTATGTTCAAGAAGAAGGACTCTTTAAAATGCATATATACATCACTAACTATGTATATATATCATATAGATTCTATAGAATTTGTTATGTACAATGGTTTTCTACATCTTAGCTAGGAAAATACGCAAGCAACAACTTTAGAACATTGTCTAATAACCAGATATTAAGAATCAATTACATATGCTGAAAGTTATCTTTTAGGATTTGTCATTTTATAGTCAGCTTAAATATGTGGCTGCACCGC

CTGCACCGCCTTGAATGATATTTATTACTTAGCTATTCCCACTCACCAATCCCAAAAATTCACTCCAGAACGAGGGAGCCACCAATGCGCTACTGAAACTGGACGGAACCAGCGTGGGAAATCGCTCGATAGCCGTGCGTTTGGCCAAGAACATTAAATATGTAAGCAAAGACCTTCGAGTCACCAAGCTAGCTATCCAAACTTCAAATCGATTCTGTTTCCTATATATATATAGGATGAAACGCAGAAGCCAAAGCCGCGCCTGGATATACCGGCCTTGGGCACAGGTAAACGCGAGGAGAAGGTCAGCAAAACGGAGGCCATTCGGGCCATTGAGGCCAAGCTGAAAATGTTGGAGCGCCAGACAGACGACAATCTCGAGCTGAATACAGCGGGCAGGGGCGAGGCCAACGTGCCCTTCATCCAGCGCTATCAGTTTAACAAGGATCGCGACGGTTCGCAGCGCTACGGAAAGTCATCGGCTCCATATCATCACCAGCAGCGTCCCAAGCGCCACTAGGCTCAATCGACCCACACTCGACTTTG

 

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