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CRIMIC CR00460

Gene CG10492

CG10492

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:19056171..19056171	37B12	FBti0199589	79263

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	OK	ATTCGAGCTGCGATTTTTGGGCACGCT	CGATCCACAGTTGTAGCAGGAGATCACT

Images

Construct Design Sequences

sgRNA sequence	AATATTGCCGAGCTAAACAATGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AAGACAGACACAGACGCCAG	TGGATGCTAATGCTGATATTAGT	GCATCCATTGTTTAGCTCGGC	GGTCCTCGCTGGTGATATGG

 

Amplicon 5'

Amplicon 3'

AAGACAGACACAGACGCCAGTGCTCCAGCATCCTCACCCGCCGTCCCCACATGACCTGGTCATTCAGCAGCAGCCACCACACGTGATCCACCAAATACCAGGACCGCAGCAGGCGCCCCCGCACCCGCAACATCCTCAAATGGTGCAGGTACTGCAGACGCCGCAGGGCAACATACCCATGATCTTTCCCCAGCACTTTCAAAAGGTAAATCTTTAAATAGAAAACCACTAAAAGTTTATTAACTTAATCTGTTCATCTACAGCTTCTGCCCAGCTCTGATGGAACGCACCAGATCAGTGTCGATGGCATGCCGCACCTGATGCAGCCTAACATCACCATTCCAGCGGACACTAGTGCCATCATCAGTCACCAGAACACGGGATGGACGATGCGTCACTACGTGCCACAACCGCCCCACCAACAGCCCGCTCTGCAGCAACAGCAGCCGCAGAGTCTGGACCATCAAGTGCCACCTGCCAGCTCGCCGATGCTGAGTCAGCAATCCTCGCCATCGAGTAGTCGCACCACGAGTCCTCAGCGGGACCGCTCCCTTATTAACGTTCCACTGCAGGCGCAGCAGCAACCACATCAGCAGCAGCAGATGAGGGGCATGTCCCAGCGAATGGCGGATCAGAAGAACACGCGGCGTCCCTCGGCGGAGACTACCCCGCCGCCAACCTCAATTGGCGGCGAGCAGCAGCACGATGTAATTATTAAACAACTAAATAATTTGTTTATTTATTTGAAGCAAACTCAAGATTTAAATTTAAAGGAAATTAACTAATATCAGCATTAGCATCCA

GCATCCATTGTTTAGCTCGGCAATATTTAGTAGTCTCCATATAATCACCATAATCATATCGCTTATACGACAAATAACTTACCTTTACAGAGTGTCAACGAAGGCGGAAGCAGTAATTCCAGCGGAACCAGTCTGCACAGACTCATCCGCAACGGTTTTCCGCGTCTAGGACACAATCATCGAGTCAAGGCAAATACCTTCATACCACAGCACCAGCAACAACAGCAGCAGCATCACTTTCAGAACCCCAACTACAACATGAACGTTATGATGCAGAGTATGAATATAAACGACGATGGCAGTGGATCGATGATGAATTCCATGAACTCCACCAAAAGTGCAGCTACCACGGGTAGTGAATCGGGCAGCTCGAATGGATCCTGTGGTGACCTGTCGCCCTCTGAGACGCCAACGCCCACGCAGCTCTCACTGCCACTGCACAATTCGGTGGATGCGGGAATGGGCATGATCACGACAGTACTGCCCACCGGTCTGGGAAACTATCAGCCGAAGCCACACCACTTCAGCTACAAGCAGCAGCAGCAGCAAATGAATCTGCAGCAGCGGCTAAACGGACGCAATGGGATGGATAAGACCTATCAGCAGGTTTACACAACGGGCACGCCAAGCATCGTCAGCGATGCACAGACCATCAGCAGCGCCAGCCTGCAGCAGCAGCAGCCCCAGACCCTTCTCATCAGCCAGTCTCCGGTGAGCACACCCACGACAACGGTGCTCCTGCAGCCGCAACAACCGCCGCCCACAACCTACAACACAAGCTATCCATATCACCAGCGAGGACC

 

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