CR00461

CRIMIC CR00461

Gene AhcyL1

CG9977

Information

Donor Location Cytology FBti Stock number
pM37-p03L:2770707..277072262F3FBti020056383175

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
no band short band AGCGCATCCTGCTTGGATTTCAGACT CATTGAAAAGCTCGATCAGGGCCAG

Images

Construct Design Sequences

sgRNA sequence GCTGATTCACCTGTATCGGCTGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
TAGAGCCTGAGCACATCCCT ATCAGCCAAGCTACGAGTGC ACAGGTGAATCAGCGTGACA ACAAGCCTCTGAAGGATGCC
 
Amplicon 5' Amplicon 3'
TAGAGCCTGAGCACATCCCTTGCCCACATCGCCGTAGCCACAGACCACGACCTGCTTGCCACCGAACATCACGTCCGTGGAGCGCTTGAGGCTGTCCAGGATGGATTCCTTGCAGCTGTAGAGGTTGTCAAATTTGGTCTTTGTCACCGAATCGTTGACATTCATCGCCGGCACTGTCAGCTTGCCGGCCTTAGAGAGCTGATAGAGACGGTGCACTCCGGTGACGCTCTCCTCGACGATTCCCTTGACCAACTTGAACATGGTCGGGTACTTTTTTAGCATCAAGTGCGTGGCATCGCCGCCATCGTCCAGGATCATGTTTGGCTGCCAGTTCTCGGCGTTAACGCAGCGATCTATGCACCACCAGAAGTCCTCTTCCGTCTCGCCGCGCCAGGCAAAGATCGGGATTCCGGATTCGGCCAAAGCAGCAGCCACTTCGTTCTAAAAATAATAAGCAACGATATTGAGTTGTTTTAATAATGACAGTCCTTGAAGGCTAATAAAATGATTGTCAAACAGGTAAAACCCTCGAGAGTTGGCCTGATAAATCGAGTCACCCTAATGCGGCGTAAACATTTGCTAATATCTGACTTATATCGAGCTGATAACGCATCGGGCTCCATTCTCGCTAAGCACGGCACTCGTAGCTTGGCTGAT ACAGGTGAATCAGCGTGACAGGCTGTCGGGGGCCGTGGGCGATAAAAGCTGGCACGGATGGGTGCATAAATGATCGGGGAAGGGCGCTCGCCCTCGCACTCGCATCGCTTCATTAGAGAAGCCATAAATTGTTTAGCGTCAGCAGACTCGTGATAATTGCATCAGCCACGCCGCAGGAATAGGCAAACAGATCGAGTAACCGCAGCCGATCCAATGCTGGAACTAAAACTGCGTCCGTAACTTCCACTCCCAATGGTATGGCGACCCAATTCAGGGCACATCAATTACTCAGCGATTGCCAAACACTTGAGCCGCGGGCGCTTAATTGATTAACATGGCTTGATTGATCATTTGTTTGCGTCGCCTGGCGTGACAACAATTGTCTCCAAGCGACCGAGCAGATCAAGTGACCGAGCACGCATATGCATATGAATTTAAATAAATATAGGAAAATGAGTTAAGATATCTCTTAAGAGATTCCATTAAATGCATTAAGCAGCCTATGAATACGTAAGAACCCTATGACATTTTCATACGACATCTCACCTGCGTGGAGTAGATGTTGCAGGCGGCCCAGCGCACGCTGGCGCCCAGTTCCACCAGCGTCTCGATGAGCACGGCAGTCTGGGCGTTGATGTGTGTGCAACCCACGATCTTGGCATCCTTCAGAGGCTTGT
 
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