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CRIMIC CR00462

Gene fliI

CG1484

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	X:21340046..21340051	19F5	FBti0199590	79264

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	OK	TTGGCAGTTCTCTCGTAATTGCTGCG	TGTAGCAGTCGCCCTCGTAGAATTTG

Images

Construct Design Sequences

sgRNA sequence	CGCACTTATGACTAATGCTCAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TCACATTCCGAAGACTGGGC	GCTTCTCGGTGACCTGTTTC	GAGCATTAGTCATAAGTGCGGAG	GACTGGTGGCTTTACTCGCT

 

Amplicon 5'

Amplicon 3'

TCACATTCCGAAGACTGGGCAAAGTATGAAAATTTCGTGTCGTTCAATTTGGTCACACCCTCTGGATTATGCTCACGATACGATGTTACTGTTTTTTCGCAGGACGAAAAGGACCCTTCGAAAAACCCGAACCCCATCCAATGTGACCGTTTTGAGCAAACTAACATAGTCCGAAGCGTTCGGCTCTCACAGATCAGCAAAAAGGAATTTATTATATTTAAGTCTTAAGGCACACATCAAATGTATAGTTAATCAAATTTAGTTTATACAAATTATATGTGTATTTCAATTTCTTTAAATAAATAATAAATAAATCAAAAACAAAAACCTTGGTTTCAATCACTTACTCCGTTAGTTTTCAACGTATTATTTGTAAGTCCATAGGACCATTTGAAACTAACGGCTCTGTTTTCAAAAAGAATCAAAAACTCCAAAACTTAAGTCGCTTTTAGCTCGGTTCCGGGAATTCAAAATAATTTTTAAATTAGCCACACCATTTTTAGACATTAACGGTCACACTCGACTTAAGCTCGTATAATACAGTGGTGGACGTACCGTTAAAAGCCATGCAAAATTAATATTTAAAGGCGAGAAACTCTATTTTGGTCACAAATGTAATTATTGCCGTATATGAACCAAATTTTTTAAGCCCACAAAGCTCCCCTTCTATATGATAAATCAGCTGCACCAAACCTACGAAGTGCAACGCGAGTGTGCCTTCTGTGATTTCCAACACTTTAGTGTTGCCTAACCCTCGTAAAATGCGCAGTGTGGCCAGTTCCGGCAACTGCATACGAAGGTGCGACCATGCCGTTTGCTCGGGTGAGCGGTGAACGGTCACCACTTAGTCTTCTTTTTTTTAGTGCAAAAAAAAAACAAAATAAAGAAGAAGAAACGAAAAACAGGGCCAGCAGCTTGAGCCCAAATATAAAACAAATATAACATTTTATGTGGTCAAAATGAGCGTGCTGCCGTTTGTGCGCGGTGTGGACTTTACGAAAAATGATTTCAGCGTGAGTACAACCAGCAAAAACAGTTGCAGCCTTGTTGTTTTTTCGGAGTGGTTCAGGTTCTAAAAAAAAAAGAAGCTAAGAAACAGGTCACCGAGAAGC

GAGCATTAGTCATAAGTGCGGAGCAGAGATAATCCCGAAATATGATTGATTCCATTGCAGGCAACATTCCCCAGCTCCATGCGGCAGATGTCGCGGGTGCAGTGGCTCACACTGGATCGCACGCAGCTGGCGGAGATTCCCGAGGAGCTAGGCCACCTACAGAAGCTGGAGCACCTGTCGCTGAACCACAACCGACTGGAGAAGATCTTTGGCGAGCTGACAGAGCTGTCCTGCCTGCGATCCCTCGACCTGCGGCACAATCAGCTGAAGAACAGCGGCATACCGCCGGAGCTGTTTCACCTGGAGGAGCTGACCACGCTGGACCTGTCGCACAACAAACTGAAGGAGGTGCCCGAGGGTTTGGAGCGGGCCAAGAACCTGATAGTCCTCAACCTGAGCAACAATCAGATCGAGAGCATACCCACGCCGCTGTTCATCCATCTAACGGATCTGCTGTTCCTGGATCTGTCGCACAATAGACTGGAGACACTGCCGCCGCAGACGCGACGTCTGATTAATCTGAAAACCCTGGACCTATCGCACAATCCGCTGGAGCTGTTTCAACTGCGGCAATTGCCCTCGCTGCAGAGCTTGGAGGTCCTGAAGATGAGCGGCACGCAGCGCACGCTCCTCAATTTTCCCACTAGCATAGACAGTTTGGCCAATCTCTGCGAACTGGACCTGTCGCACAATTCGCTGCCCAAGCTGCCAGACTGCGTCTACAATGTGGTCACTTTGGTGCGACTGAATCTGAGCGACAACGAGCTAACCGAACTGACTGCCGGTGTAGAGCTTTGGCAGCGTCTGGAGTCGCTGAATCTGTCGCGGAATCAATTGGTTGCGCTGCCGGCTGCCCTCTGCAAACTACCAAAGCTGCGACGATTGCTGGTGAACGACAATAAGCTAAACTTCGAGGGCATCCCCTCGGGCATCGGCAAACTCGGAGCTCTGGAGGTGTTTTCGGCAGCCAACAACCTGCTGGAAATGGTGCCAGAGGGACTCTGCCGATGCGGTGCTTTGAAGCAGCTCAACTTGAGCTGCAATCGGCTTATAACCCTGCCCGATGCCATTCACCTGCTGGAGGGCCTAGACCAGTTGGACTTGCGCAACAATCCGGAGCTAGTGATGCCGCCCAAGCCAAGCGAAGCGAGTAAAGCCACCAGTC

 

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