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CRIMIC CR00463

Gene CG8032

CG8032

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3R:8664885..8664897	85A5	FBti0200564	80585

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	no band	TGTTGAACAGCAGACTGTGTCCGATG	CATTTGGTCAGTATGGACTGCTTGGC

Images

Construct Design Sequences

sgRNA sequence	TGGAAAAATCTATAATCAACTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCTCGCTTTGAAATCGCGAT	GGCTGGGGCGAAGAATGT	GATTTTTCCACGGACTGCGC	ATGTTGTACGGCCAGCTCAA

 

Amplicon 5'

Amplicon 3'

GCTCGCTTTGAAATCGCGATTACGTTATTGCATTGCACATCGCATGCATTTATTTACAAAAATTATGTTTTTATCAGCGCGACGAGTAATTCGTGTACATAGGTCGTTGGAACTGTCTGGCAGCTCTGTTAGTGCTGGCAGGTGGTCGATAACAAGTGGAGTGTGATTAGAGCGATTACTTTTTATTTCAAATACTTTGTAATAAACTTGTTAGTGTCTTAATTAAAACAATTCAAATCTTCAATTTGAAATGTATATTAAAATCAAAAGTTTCATGAAATCACAGACGTTAAAGTATCGATACATTTAGTGCTGTAAAATGACAGCTGGCTTGTTTACCCTTATAAGCTTCAAGTCTGGCAATACTGCGACCAGCAGCTGTCGTAAAACTTATCCTTCAAACTTTCAATTAAAATACGTTTTTCTGAAAAAATCGCTTATCAAACTCCCATACATAAAAATAAAACTATTATTACACAATGTTTACTAAAAAAAATTGGATATTTACCACCCAGCCGAACAGCTGTTCGCTGCTACCTCCCCCAACCCATAAACCTACTGTCAAGTAAACGTCAAAACAGTCGCGCGACTCGCCAAGCGAAAAAACCTGCAGAAAAATTGAAATGCGGCGTGAAAGATACTAGAAAAATAACAAATAAACACCGGGTATCCGCAATTAAAACGCTTATGTACATAGAAGCCAAAGTGTAGCAATGGGCGACAAGGAGGAGCCCGTAACACCCGCTCCCTCGCCCACGGAGGAGGGTATGGCCAATATTGGGTCTGCTGGAGCCGGGGATCAGCCGCAAACTTCAGGGAATAATACCAACGTTAAGATCGTGATCATTGGAGCTGGAATGGCTGGCCTATCGGCTGCAAATCACCTTTTGCAAAACGGCTGTGACGATTTCCTTATTCTGGAGGCACGGGGCCGGGTGGGCGGACGCATTGTGTCCATACCGCTCAGCAATAATCAGAAGGTACCATTTGTTATATAACCCCCTCAAGTCCAAAAACCAAAACAAACCATCATAGGCGACCATATGGTATTAAAGGAAAAACACTTCCGACTCGGAAAAACCACGAGCATGTTGTAACACATTCTTCGCCCCAGCC

GATTTTTCCACGGACTGCGCAGTCGGTCAGCTGTTCACGCTTTTCATTAAAGCTATTTTTATACATGTTCCTGTTCCCTCTATTTGCCTATTGTTAGCTTTATTTGTTACATATGCTTACATGGGGGTAATTAATGTAATTGCATCTGTCGTGAATCTTACTCACGATTTTCATTCCGTTCATTAGCATAGTTTTGATCGGTTTGGCGGCACCTTATCAAGAAATTTGTTATACAACTTTCTTCGGCAACGTTTGTCAGATTAGATAGGGGTTTTCTGAATTAAGCCAATGATGACGTATCTAGTGCAATGCCCTGAAAAACTCACGGGTTTGCGGGCTAACGATCACGTAGTCTTAGTTATGGGACTGAAAAATTTATTATTTTTCCAGTTTTCCACATTGTTTCACTTCACATGTACTAATTACGCCCCTAAATTATCTAATATAGGTCAGTTCTTATCTTTTTTATACTAGAAAGTTAAATGTTAAGCTTTGCCTACTTATCTGAGATGTTCCTATTTAAACACTTCTATTTCACAGATAGAACTTGGCGCCAACTGGATACACGGCGTTCTGGGCAATCCCATCTTTGAGCTGGCCGTACAACAT

 

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