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CRIMIC CR00467

Gene PI4KIIIalpha

CG10260

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	X:2625390..2625403	3A8	FBti0199591	79265

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	short band	GTAGATGAAGAGATCGTGGGCGATCAT	AATTCAAGATCGTTTGCTCCGCGGC

Images

Construct Design Sequences

sgRNA sequence	AGGCACAACAGAGGTGCTGTAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AGCCGATTCACTGCCTTTCA	ACCTCTGTTGTGCCTGAATCA	AGTTGTCTTCACATCCGCTGA	GGACACCGCGAATATGGTCT

 

Amplicon 5'

Amplicon 3'

AGCCGATTCACTGCCTTTCAAATGCTCTCCCAGCAATTCGAGTTCAGAACTACGAGTGGCGTGAGCTAAAAGGCCAGCTAGACGCGAAACAAAATTAGAATAACTTTTGGAATGTAACATTTTTCAAATGTGTAACGTATTTTGGTCTGCATAAGGGTTAATTCGAATTTTTCGAGACACGACATTCGGTTAGGCGGCTCATTCTCGCGCACTTCGCAGCCAGCGCCACATTTGCTAGCCAATGCGCGCTGGTCACACTGGCGAAAGTAAAATAAAAAAAAATTAGTTTTATTTAATTTCTTGCGAACGAGCCACGAAAATGACGAACGCCTAGCGCGATTGCCCAAAACGACCCACTGAATCAACCGCACCGCACCGCAGCGAACCGCATCGAAACGAACGAACGGAGGAGGAGCAGGTGGCCGCCAGTTAGAGCAGCCATGACGATGACCGCCTCGGATAAATACACGTACCAGCGGACCGTTCTCTGCCTGGCCCGCGTTCTGGCGGGCATCCAGCCCACTCCCTGGGATAAGGTACATGTCCACCTGGCCACCCACTCCACCAGCCACAGCGCCAGCCCCCCTGGCAATCGACTTTTCGCACTGACCTTGAGCGGCGGCCATAAAACAGACATGCGTGGGCGCTCAGGGGGCGGCGGTAAGGCAGTGAGGCGGTGGGGCAGGAGCCCCACACCAAACCAACTCAGTTTGCAAAAGTGCCATTGGAATGTCAACAGTGCAGCGAATCTTCAGCTGTTGCTCACTGAAGCGCTCACACGAAAGCTGCTGATGATTCAGGCACAACAGAGGT

AGTTGTCTTCACATCCGCTGAGGTCACCAGCAGTAGAAGCTATTAGTTAGGCGTATATCATGAGTAGAGTTGTATAGGTCAAGGAATAGGAATAGGAATAGCACTAAGCACAAGACACTGAGACACACACCCCACTAATCTATGGCTTCCTCATGCTACACGCCCACAGGTGCAGACCCTGTTCCGGTATTGTCCGCAGGAGAATGCTGCCGGAGTGTTTTGCCTGGACACGCGGGCCCAGGATGCCGTAATTGCGCTGGGCATTTATTTTCTGGAGGGCGGCTGCCAGCACGAGGGCCAGATTGTGCCCTATCTGCTGCGTCTGGCCAAGTGTCTGCCGAAGGCGGTCTGGATTGACGATGCCCGGAGCAACAAAGTGGAACGTAAGTACTAGCTGCTTGCCACTCGTGTGGCCTGTCGCACAGTTGGTTTATCCTGGCTTCCCCACTTCCATTTGGCAGGCGTTCGCATTCCGTCGGCGGAAAAGTTCAGCTTCTGCTTGAACACCCTGCTGTCCGACATAGCGGCCAAGTGTCCGGATTCGCGCGAGGAGATCATCCTGAATCAGGTGGAAACGCTGAGCGCGCTGGCCAACATAGTCAAGTCGAGCAGGGACAGCAGCTCCGCACCGCCGCCCATCATCCTGTGCAAGGCCACGGTGCCGCTGCTCTTCGGCTTGGCGCGATCGATGGGTCGCTATGCCAGCAACGATCCACCGCTGCTGTGCCGCATTTTTCCGCCCGAGCTGCTGCCCATCCAGAAGGGCGGCGGCCGCGACGGCACCGGCTCGAGCAGCAGTGCCAGCGGCACCTGCGGCGGCTCCTTCAGCAGCAGCGAACGCCTGGCGGCCACGCATCACTTCCGACCCATCATACCGCGCTCCATGTCCGGCAGTCTGGCGCAGGCCCAGAACGCAAGCTACGACGATGGAAGGCAGCGGTGCGCGGGCGGCAAGCCCAGCAAGCCATCGTTGCACAGCTACTTTTCGGTGCCGTACGATCCGCGGACGCACTTCTTCACGCGCTACGGCTCCAGTTTCAACCAATTCCCGAACATGCGCGTCTGCGAATCGCCCACGAAAGGCGGCCCACGACCACTGTATCGTGTGCCTCCGTTTCCCATCCAGCATCTACAGACCATATTCGCGGTGTCC

 

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