• About
• Search
• MiMIC
  • Technique
  • Constructs
  • RMCE Lines
• Transposons
  • GDP
  • Others
• CRIMIC
  • Technique
  • Constructs
• Order
• Downloads

CRIMIC CR00471

Gene Atac3

CG32343

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3L:326644..326644	61C1	FBti0199382	78921

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATAGGCCTTCACTCGTCCCTGAAAGT	GGGTGCTTTACAAATGCACTTATCGGC

Images

Construct Design Sequences

sgRNA sequence	AGCAAGAACATTCCCAACTTTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GACTGCTTCGTCGCATTGAC	TGGCCTACATTCCTGATCCT	CCAAAGTTGGGAATGTTCTTG	GGTCACGAGCGGATAGTCAG

 

Amplicon 5'

Amplicon 3'

GACTGCTTCGTCGCATTGACGATTGTCTTGCCAGGTGCCGCCCCGTTGTTTACTGCGCCTTGGCCGGATCCTGCCGAGAGTGAAGGTCGCAGCTGTATCGTCTGGACGGTGCCCACTTGTCCCCTAAGCGAACTGCCCGACGCGGGATGGCCTGAAGGCAGTGGGGATGGGACCGCGGCTTCGTTCTGGCTGTGTGATTCTTCTAGCGCAGTGAGATCCTGCGGCCTTTGCAGGGAGGGGAGCTTTAACTAATTAAGCGGGGTTCAAGTAAAGTAAGGAGGTGAGCTCAGAAACTATTTAATATTCACCTGTCGAGCCTGCAACTGGCGCATGTTGACTAGCTTTGTGCCATCGGGAAGATGCCGCACCATTCCAGAGCTGTCGATAAGGCAAGATCTTATATATATCTCCCAAATATGTGAAATTAACGTACCTTGCCAATGAGGCGGGTATTTTCTGAAGGGACTTTGCCGTGCTGTCCGATCCGTAGAGTTTCTTAAAATCAGTCAACGAGATAATGCGTATATTCTGCCCTCAAATATTAATTATTATACGAATTAGCGAAACAAAACAATGAAATGCATACCTTGTTCTTGGCAACTCCCGCCGGTGACGGAAGATCCTTCTGCTTCAGGACATTCATCCGAATCTTGGGATGAGAATTATTAACATTTGTGCGGAGCGCTGCGACCGAAGGCCGGGCGTTTCCGTTCGTGGATTGCTTGCCGCTGATGCCCGATTTTGTTTCGTGCAGCAGCATGCGTCCTCCTTCAGAGAGGACAAGTTGGCGTCCATTCTGCAACGCAGTGTTCAGCATTTCTTTCGAGTCCTCGTCATCGTTGTCCTGCACCATGTCTGCGATTTCGTGCGTCTTTAGTATGTAAAGCGCAGTGTTCCGCAGGACGTTTGCATCTGGCAGGGAAAATAGGTTTTTCGTTAAATATTCGTTTAATATCTCCGAATCTAGTAACTTACGGCCTCGCATGTTTTCTAGGACATCCATTCGTTCTTCAACCGACATTTCTTTGTCGTCCACGGTTTTAATTACTTCCGGTTCGTCCATTATTGAGCTGACAGCTTCATTGGTTTCCTTCTGCAAATCGATGGGGAATAAATATGATATGATTTTTCTTTCTGCGAATCAATTGGGAATAAAGTATTTGTCACAATTTTTTAGGATCAGGAATGTAGGCCA

CCAAAGTTGGGAATGTTCTTGCTATTATTTGTGACAACCTAATACAAGTTGTCGAAAGTACATACTTTGGTCTTATGTGCTTGTTGCTGAAGTTTGATAAGCGCAGCGTGGGAAAAGGCAGCGATCAATTGTATTGTTAAGAATAAGGAACAGCCAAGCGAAACGTAAGTAAAACCTCATATTAAGTAGTAAATATGGCCTTCAGTGGATTTCTTCAAGCGCACTCACCTCCGATTCCTCGTGTAACTTCTTACTGGCCTGCGCCTGTCGTGCCGCTTCCAACTCCGCCAGCACATCCGCTTGTTCCGTGTACACGGCAAGTCCGATCGGCGTCTTGCCGAATTTGGAGACCAAGGTGACATCTGCCTGGCACTTGAGTAGCAGGCGCACAATTCCCTTGTGTTTTTTCTCGACCGCCCAGTGCAGCGGGGTCATGCGAAGCTGACAGGAATGTTGGTTAGTCATTTTAAACAGTCATTTATTTATATATTCTTACCATGTCGCGGGAGTTGACGCTGCACTTTAGCGCTAGCAGCAAGCTGACTATCCGCTCGTGACC

 

---

Search Again