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CRIMIC CR00473

Gene CG2926

CG2926

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3R:5585104..5585104	83B3	FBti0199592	79266

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TACTACAACTACTCGGCGCACAACCA	ACTCTCGGGCTCTTAGAACGCATCTT

Images

Construct Design Sequences

sgRNA sequence	TAGTAGGAGTATTTAGTATACGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GTCGCTTAGACGGAGCTCTC	ACGGAACCGAAGATTTTAATGT	GGTTCCGTATACTAAATACTCCTAC	AGTGCATATTAGGGCGGACG

 

Amplicon 5'

Amplicon 3'

GTCGCTTAGACGGAGCTCTCTTGGGGCTGACCTGTGCCTCCGTTTCCGTAAGCAGTGCCTTCTCGAACTCTTTCGGTTTGTCAATGTCTAACTCCTCCCAGTCGCTATCATCATAAAGCTCGAGTACTCCCTTGGCCCGATCTCGATCCGCAGCGCGATCCTCTTTGACCAGTGGGCTGGGAGTGCTGCGCGTCCTAGCTTCCTCAGCATCGTTTTCCTCTGGGGCTGCAGCTTGCACGTTGGAGTTGCTTGGCTCGTGATCTGAGGACTCGGACTTCTCCGTTGGAACAGCGTCACAGGACTCTTCGAACATTTCGGGTTCTTCAGGTTCTTCGGTTGGATTCTCTGGCTCGTAGAGGTCATCATCACTACTTTTCGGGACTTTTTCGGGAACGGGTTCGGGCTCAGGCCCCAAAGGCAATGGTATATCCGTATCCTCATCATCATCATCTAATTGTACTAAATCTTCATTCATATCGTCATCATCCTGTCCCAGAGGAAAGGATTAAATGAAGATGAAAAGAGAAGATTAGAGATTAGTAAGCGTTGAAGAAGAATTGGTCTGAAAAACTATCAAACGTGACTTACTTAACTAATTATATTGCTTGCTTTGGTTTCAATAACATTATATTTGGTTTTAAAAACTGAAAATTAAAATGTTATACATTTGTGTATCTTTTTGTTTCGGAAGATTATGGCGAGAATAATGATGTACCCATTACTTACCTATATAATTTTAAATATGCTTTTTTGTTTGCATAATTTTGCCTTTTATTACTGGTAATAAAGTTAATAACTACCCACTTCAAACATTTTTCATCACCAAAGCTGATATCAAAATCACTTTTTTCTTTACCTCGCTTAATTTGTTTGATAATTACCTAAATCAACATTAAAATCTTCGGTTCCGT

GGTTCCGTATACTAAATACTCCTACTATTATTCATGTTAAATATCTCTAGATTCTGAAAACCCCATAGATATCATACCTTATCAGATGCATCCGCTGGTCCGTGTGGAACCCCTGACGGCATCGCCGAGGCATTAGCAACCGCCTGCGACTCCTGCGAGTAAATTGAGAAGTTTGGGCAGTTGTCAACATCGTCGTTTTGGTTGCCATCGTTGGATTTGGCCGGTGCTTGCTGGAGTGGGGTTATCTTGAAGAGAGGACTGTTCCATGGAAGGCTGGCAGGTGGCATAGGCGGAGGTGGTATGGACAGCACAACTGGCATACCCATCACTGGTGCAGGATGATGATTGAGGGGAGCTGCAAACATTGGAGTCTGTCCCGAAAAATTGTTTGATGACGACGCTACTGGGGCTGGAGGCGGTGCACTTCCAGGGAAAGGCGGGGTGGGTTGGTTAGCTGGCAGCACGTTTTGCTGTCGTGGTTGCTGCTGGCGGGTGGGCGTGTTGAATCGCAGATGGAACGGAATAAAGCTTGACATATTAGTGTTGTTGTTGGAGCCGGAGCTGCTAAAGTTTTGGTTGCCAAAGCCACCGCCTCCTTGGCCGTCTCCTTCACCGGTCAAGTGTCGCGAGGAAGCTTCCCCGCTACTGCTGGGCACACCACTACCACCGCTAGATCCTCCAGCGCCGCCTCCACCACCTCCGCGGTAACTGGTATTGCCGCTGTAACGATTGTTGTAACTATCACTTGAGCCTGCTCCTCCGCCTCTGTTGTAGTTAGGTCCTCCTCCTCCCTGTTGGTAAAGCGGTGCCTGGGTTAAATGCTTCTCGGTCTTAGAAGTACCTGCTTTCGGTAAAGGCGCTTCCGTCGCTGTAGGTGCAGGTCTCTGGGGAATATTGAGGGTACCGTCCGCCCTAATATGCACT

 

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