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CRIMIC CR00474

Gene Coq4

CG32174

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3L:17429764..17429782	74C1	FBti0199593	79267

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	no band	ACAATCCAGCAGCGTCATCACTCAAC	TTGTTTCAGCATCTGCATACGTGGCG

Images

Construct Design Sequences

sgRNA sequence	TTCACTGTCCAAATTCTCTTAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GGAGTTGCCACATCGGGTTA	ACAGTGAATGTCATGGTCGA	TGTCTTTGAACCAGATTTTCGTCG	TGACGACATTGAATCGGGCA

 

Amplicon 5'

Amplicon 3'

GGAGTTGCCACATCGGGTTAAGTTTTGATGCGATCATTCGCGGAAATTGACTGTTCTCACTGTGCTCAAATTCTAATATAAAAAATAATATAAAGTTTTTTTCTCGATCTTTAAATTATTTCGGCAAACTGCATCAACTTTTATATTTATTGCTATAATCAAATAATAATTTTCTTTGTAAGTGGAAACATCTACAATCAGTTCGACGTTACACGACGTTGCCAGATCACAATTCTACGATCGCGATAGGACTGCTCGATACTAATAGTCGATAAGTTCAAAATTTAAAAATGGGCTTAAAAATAAACAGCCTTATCAAGCTTTATTCAGCACAGTAATGCCCAATTCGGAGCGCAGCTCGTGGATGTTCTGCTCCCAGCGCTTCTCCCAGTAGACGGGCATCAAGGGCTTGGTCCGCTTGCCGTTCTCCAGGGCCCACGGCAAGTAATGCTTCAAGTACGCACGCCGCTGCCTAAGTGGAGGAGAAAATAAACAAACGCGTGAATGGGGAGCTATAATTAGAGAACCCAGTTCACTGAACTCACTTGGGACGTAGGCGAACTGCTCCGAATACCGCACCACCGTAGCACATGGGCAGGCCCGTGTTCAGAGCCTCCACCCACTTGACAGCCACCTCGCCCAGCATATTGGTGGGCATGTCCAGCACCGTGTGGATTAGGTCGTGGCATTCGCGATATCGCGTCATCAAGTAGGCCAACTTCGGGTCCTCCAAAAACCGCACGGCCATGCGACTGTCCGGCGTGACTTGCTGAAAATTGATTACCAAAATAATCGTTGAAGCGATGGACAAAAGCAATTATTATTTTATTTTCCATCTAAATATATCTTTTATATGCAAGTATGTTTATGAGACAATAATTTTTAAGATTTTCAATTGTTACCAACTTCAAATTCGACCATGACATTCACTGT

TGTCTTTGAACCAGATTTTCGTCGTTTTATGGTTACTAACGCTTTTTCTTACCGGATTCTTTGAACTACTTACATTATCTTTCAGAAACTTTACGTAAGCGGCTCCAAAGGTATCCGGTGGCAAAGTTTCCAGATACTTGAAGTCAATGGTGCTGGTGTGGATGCGGGGCTTATCAGCCATGATGCGCTGACCCTCCTCGCTGGCTTGCATGGTGTCCAAAATTGTCCATAGAGCATCCTCTCCAGTCGTCTCACCCAAACAGGCGATCATGTCATGACTTCATAATAATAATTAAGAGTTTTAAGAGATTTTTAAATTTCATCAGATGCCCTACCGTCTTGGATTCAAGAGCGCGGCAATGGAGGATCCTGCCCCCAGGAACAGCCGCTGGAAGGGCGATATCTCGATTCGCTCCTTGAGGTACTGACGCTCGAAGGCATCCAGAGGTTCCGCCTCGGGTGCTTCTGTGGCCACCGCTTGGCTATTAGCCTGGGCCAAGCGAAGGCCTCGTCGTAGGGCCAGCGGCTTTTGGAGACGCAAACAGCGTTGCATCATGATGATCTGTGGATTCATAGAGGAGATATGGGGAGGTGCACCCGGTGGTGACCTTATCAGCACGATCGTCGGCATCATCATCATTACATTATTATTATCATCATCATCATCGTCGCACACAAACATGACCCTGACTACCTGTCTTCGGTTAAAGCTGGTCCCTCGAGCCATACGTACGTATGTACGTGCCCACTTTCAATAGCGGCGAATATGAGCAAACAATTTGTCCATAATGTGGGTACGCGGTCGGGGCTGATGCTGACTAACAATTTACCTCACCGCACGGCTAAGTCTGTGCAAACCTGTTTCGGCTTCCTCATTACCTGTCCCCACTCCGAATCCCTCACTTCACCACCGCCCATCATATATTGCGGGCTCCCAAAATAACCCAAACAAAGTGCTGCCGGGGCACTAGCACCTCATTCTGCCGCTTTATGCCCGATTCAATGTCGTCA

 

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