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CRIMIC CR00477

Gene Kul

CG1964

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3R:29729172..29729172	99B9	FBti0199383	78922

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TAAAACCGCCTTCCTCACGCAGAGAT	ACTGAAGGTCTAAGTGCTCACCCAAG

Images

Construct Design Sequences

sgRNA sequence	TCAGAAGATGAAGTCAGCACAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GATGATTTTCGCGTCCTGGC	CAGGATCTCAAAACCGGATC	TGAGATCCTGTGCTGACTTCA	ACACAGCTCGCATGACTTGA

 

Amplicon 5'

Amplicon 3'

GATGATTTTCGCGTCCTGGCCTCCGAAGAGGACAAGAGCGAGGAGTCGCCGCGGAAGTACCCGACCACGTCCACAACCAGGAGCACTGTGCGCAGCACCGAGAGCTTCCTGGCCACTCTGACTAAGCCCACGTCGAACCGCAACATACTTGTAAATAACTACAATCCCTCCAATCTCGGCGAGGGAAGTCGCAGCTACAGCAGCGGCAACAACAACGCCAACAGCAATAGTAACAACAATAACAGCAACAACAACAATAATAATAATAATAGTAATAACAATGTGCGGAAGTTGTACACGAAACACAAGAACATTATTGTAAGCACGTACAACCGACCCATCGAACCGGAATTCGGAACGCCCTACGAGGAGCCGAATAACAATGGCACCCGCGAGCTGCCCAGTAACTTCTTCAATGCCAACTGGACGACGCTCTTTCTGGGAAACAACAACAATGGCAACGACCGGCCCAGCCACAAGACGCATGTGGAGATCATCACGAAGAACGGAGCCACCAAGAAGCCAAACATCATTGTGAACAACTACAATCCGGAGATCATATTCGCACCCAATCCGCACAATCCGAGTTTTAACAGCATGATGATGACAAACCTGCTGAGTGGAAGGGGTGGCGAGGACATTCACAGCTCTCCCAGGCTGCTCTACGACCGCAAGACCACTTGCATGCTATACCTGCAGGCGGACCACACGTTCTTCCAGAAGATGGGCTCGGACGAGGCGTCCATAGAGGCCATCACTCGGCACGTACAGCGTGCGAACACAATCTACCGGAACACGGACTTCAACAACGATGGGAAGCCGGACAACATCACCTTCATGATCAAGCGCATCAAGGTGCACAACATGAACGCGATGAAGGATCCCAGCTACCGATTCCCCGGTAACTACGGCGTGGAAAAGTTTCTGGAGCTGTTTTCGGGTGAGTGAACTTAACTTTCTGGATAATTGATCCGGTTTTGAGATCCTG

TGAGATCCTGTGCTGACTTCATCTTCTGATTAATTGATATGCAATTAACATTATCTGTGATTTCTTGATACATGTTTAATTAATTATATTTGTGTTGCGTGTATAATTTATATGAACTTTTGTTGATTTATTTTACAGAGGAGGACTACGATGCCTTTTGTTTGGCTTACATGTTCACCTACCGTGATTTCGAGATGGGAACCTTGGGATTGGCCTGGACGGGAGATCTCAAGAACGCTGGCGGAGTCTGCGAAAAGAATGGTCACTACCGGGGGTCCCTTAAGTCACTCAATACAGGAATAGTCACTCTGTTGAACTATGGAAAGCATGTGCCACCGGCTGTCTCCCACGTGACTTTGGCCCACGAAATTGGACACAACTTTGGATCACCAGTGAGAATTCTATAATTGTTTATAAAATATTACACGTTGTTAAGAGTACTTTCCAATTTCAGCACGATCCGGAGCAGTGTACGCCTGGTGGCGAGGATGGTAACTTCATTATGTTTGCCCGCGCCACTTCGGGCGACAAGAAGAACAACAATAAGTTCAGCACCTGCTCGCTGAAATCAATAGAGCCCGTGCTGAATGCCAAGGCGCGTTCCATGAAGGGCTGCTTCACCGAGCCGCAGTCATCGATTTGCGGCAATGGCGTGGTGGAGCCGGGCGAGCAGTGCGACTGCGGCTGGGAGGAGGACTGCAAGGACTCGTGCTGCTTTCCCATGTCAAGGCAACCGCGTCTGGATGAAACTCCGTGCACGCTGACGCCGCATGCCCGCTGCAGTCCTTCGCAAGGTCCCTGCTGCACCACCGATTGCAAGCTGAAGTTCGGTGACAAGTGTCGGGATGATAACGGCTGTCGGGATCCTTCATTCTGTGATGGGCGAGTGCCCCAGTGTCCGCCGTCGGTGAACAAGCCCAACAAAACCATCTGCAACAAGGAATTCGTCTGCTACATGGGCGACTGTACGGGTAGCATCTGCTTGGCCTATGGTCTGGAATCGTGCCAGTGCATTCCCGGACCGCAGGACGATCGGATCAAGTCATGCGAGCTGTGT

 

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