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CRIMIC CR00478

Gene Su(var)3-3

CG17149

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3L:20229012..20229012	77A3	FBti0199594	79268

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	no band	ATCCAATGACGATCCTCAGCAAGCAG	TCTAACCGAGTGCACTTCCGAGCTTT

Images

Construct Design Sequences

sgRNA sequence	GGGCTGTTGAAAGTCTTTCCAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CAACCAGCGGAAACCCAAAG	CAGGTCTGGTAATGGATGAAT	CCTGGAAAGACTTTCAACAGCC	CTCATTGGCGGGTAAGTGGT

 

Amplicon 5'

Amplicon 3'

CAACCAGCGGAAACCCAAAGGCACGGATGGCGATCTGAAATACTGTTACCAAGAGTTTAACATTCGGAACACCCAGATCAAAATGGAGGAAACGATCAGTACGTTTCACGATCTCCATGCCGAGGAAAAGCAGATGTTAGCCAAACTCCACGAACTGGAGCAGAACCGACCTAGGTAAGTAGCCTTTCAAGTAGTCTTTGAAAGCTTGAAATACGTTAAATGGCCTAATTAACTCCATTCGTTCAGCGATGTGTACCTCTCGTCCCGCGACCGCCTGATCTTGGACTGGCATTTTGCCAACTTGGAATTTGCTAATGCCACCCGACTAAATAACCTGTCGTTGAAGCACTGGGATCAGGACGATGACTTCGAGTTTATTGGCCACCACACAACTGTGCGGAACGGTTATTCATGTGTGCCTGTAGCTCTTACTGAAAACCTGGACATCCGTGTCAATAGCGCCGTCAAGGAGATAAAGTACGGCACCAAAGGTGTGGAAGTAGTGGCTGAAAACTTGAAGACATCCAATTCGCAGATGACATACAAGGCAGACCTTGTCGTCTGCACACTTACTCTGGGCGTCCTCAAAGTGGCGGTAGCACATAAGGAGTCACAGCAGAGTAACACAGTCAAGTTCGACCCGCCATTGCCGGACTGGAAACAGCAGGCCATCAAAAGGCTGGGATTTGGGAATCTCAATAAGGTTGTGCTCTGCTTCGATCGCATTTTTTGGGATCCCAACGCTAATCTCTTCGGACACGTGGGCAGCACGACGGCGAGTAGAGGTAAGTGATGTGACTTTTGGCATTTGCTTATCCTATTCATCCATTACCAGACCTG

CCTGGAAAGACTTTCAACAGCCCAAATTGTATTGGTAATTAAATGTGTTCAACGTATTAATTGCAGGAGAAATGTTTCTGTTCTGGAGTATAAGCTCATCTCCGGTGTTGCTCGCTCTAGTCGCCGGAATGGCAGCGAATCTGGTGGAGAGCGTGACGGATGACATCATAATCGGGCGTTGTATGTCGGTGCTAAAAAACATCTTCGGGAATACCTCTGTTCCACAACCCAAGGAAACAGTGGTAACGCGTTGGCGCAGCGATCCCTGGGCCCGTGGGTCGTACAGCTACGTATCCGTTGGTTCCTCGGGCAGTGACTACGATCTGCTGGCGGCGCCGGTCATTCCGCCGTCCAGTAAGGATGCGGAAGGATTGCCACGGCTATTCTTCGCGGGTGAGCATACGATACGGAATTATCCGGCCACTGTGCATGGAGCGTACTTGAGCGGATTGCGCGAGGCGGGACGTATTGCGGATTACTATCTCGGCTATCCAGAGGGAACACCGCCGGACATTGGCTACTCGGTGGCTGAGGCGGCCAATCTGGTGTCCGTGGGAAATGTTGTCAAGCTGCGCGACCTCTCGCCCAATCTATCTGACTCCTCCCCGTCGTCAAAGAAGTCGGAGGAGAATTCAAACTCAAACACTGCCGACTCTACGGAGCTACAGTAACAATGTATTTAGCGTGATGGATTTGGTCCCCAGCAGTTACTCCTAGAGCGTTTAGTTTTGTATCATACAACTGGGGCTCGCTGTTTAAAACGAAGCTAGGCCGCTGTAAATACCTAAGACTATTCAACTAAGCATAGACCACTTTCATTCAACCAATTATAAATTAAGATAAAAAGCAATAACACCCAACAAAGCAAATCCTCTTCAGTGTTTCCTTCAGATCAAAGTAAACCAACCTCTGTGCGCCTATAATTGATCAACAAATGCAATCATCTATCATAACTTTCCGTACCACTTACCCGCCAATGAG

 

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