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CRIMIC CR00481

Gene CG17829

CG17829

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	X:626394..626400	1B12	FBti0199384	78923

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGCTTCCCACACGCTTAGAGGGAAA	GTAAGAACCGAATACAAAGGTGCGAAACG

Images

Construct Design Sequences

sgRNA sequence	GGCTATAGATATGGCAGCAAGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCTGTGCGTGTTATCGGAAC	ATGTGTTTTTAAAGAGCAAGTCT	AAACCCTTGCTGCCATATCT	ATGCGCTCGAACTCGGTATT

 

Amplicon 5'

Amplicon 3'

GCTGTGCGTGTTATCGGAACTGGACATATATTTCAATTAATTGATGAAATTATAAGAACAAATTTATATGAAATTATAATTATGTTGTTTCTAAAAATCGCTTCTCGAAGCGAACTTAAAGTCATCCTTAACAGCAATGTGTACCAGCCAAAATAAAAAAATGTCGATTTTGTCGATGGAATTTTAATGACTTTTCGGAAAAAAAAACATAGATAAGTTGAAAAATATTAAACCACTAACTATTCAACTAATTCAAAAGGAAGAGGAATTTACCATCGATTTCTTGAGCGACCTTTACAAAAGCGATGTATCGATGAAAAATCGATTGGACCAGCTCGTCGATAAAGTTGTTGTTTGCTCCAGGTTTACTTTTAATTTCAAATTCAACTAATATCCGGGATTAAGAGTGTAAATCCTGTCAGGAAAACCATGCTAAGGCCCCAGTCATCAGTGCCTGCGCCTCAGCCGCCTGGCAAGCGAAAGAAGCCCGCGGAACTGGAACTGACCTGCGGATGGCGCGACTGCCAGGAGATCTGCACTGGCGAGTGGAGCCTGAACGGGCATATTGGGGATCATCTGGAGCACTATGCGAAGGCCCAGGACGATCGGGGGGCGCATGCGGAGCACACGGAGCATCAATGCACATGGAACTCCTGCGATTTCCGCACCGAAAACCAGGTGGAGTTTGAACGGCATTCGTACTACCACGGCTACTACTTGAACCTGCTCCTGCAGGGGAAGCTGGAGTGCGATCTGCATCCGGAAATCCCCGCCTGCACTGCTCCGGCCCGCCTCATGGAGAAGCTGCCCGCTCTGGGCCAGAACTTTCGATGTGGTTGGACCGACTGTGAGAGGGAGTTCGTTTCGATTGTGGAGTTCCAGGACCACATCGTTAAGCACGCCCTATTCGAGTACGACATACAGAAGACGCCGGAAGACGAGCGGCCCAAGACGATGTGCAACTGGGCCATGTGCCACAAGCACATGGGCAACAAGTACCGCCTCATCGAGCACATCAGTACCCACTCCAACAAGAAGCAGGTGGCTTGCTTCCACTGCGGCGAACTGTTCCGCACCAAGACTACGCTCTTTGATCACCTTAGACGCCAGCCGGAAAACAACAGTGAGTGCGAGACTTGCTCTTTAAAAACACAT

AAACCCTTGCTGCCATATCTATAGCCATACGTTATTCCTGACAAAACCCGCAATGATTTAATTTAGTTTACTTAACTACTTGGCCACCCTCTAATCTGTTACAGCAAACAGCTTCCAGTGCGCCCAATGCTTCAAGTTCTTTGCCACCAAGAAGCTGCTGAAGAGCCACGTGGTGCGACACGTTAACTGCTACAAGTGCACCATGTGCGACATGACCTGCAGCTCGGCCAGCTCGCTGACCACCCACATTCGGTACCGCCACCTCAAGGACAAACCGCTCAAGTGTAGTGAGTGTGACACGCGCTGCGTTCGGGAGTCGGATCTGGCCAAGCACGTTCAGATTGTCCACTCGAAGACGGTGCACCAGTGCGAGCATCCCGACTGTCATTATTCTGTACGCACCTACACGCAGATGCGACGGGTAAGCCACCGTTCCCCAAAGCTGGATTCCTTTCTGCCATTCGCTCGACCGCAAGTAACCCTCCTGTTTCTCTTACAGCACTTCCTCGAGGTGCACGGCAACAATCCCATTCTGTACGCCTGCCACTGCTGCGAGCGCTTCTTCAAGAGCGGCAAGTCGCTCTCCGCTCACCTTATGAAGAAGCACGGCTTCCGCCTGCCTTCGGGCCACAAAAGATTCACCTACCGGGTGGACGAGAACGGGTTCTACCGCCTCGAGACGACGCGCCTCGAGAGCTTGGAGGTGACCCAGCAAATACTGTCGCCGCAGGTGAACGACTCGCTAGCGAAACCGGGCACAGGATCCTGCTACGAGATCGTGGATCCCACAAATACCGAGTTCGAGCGCAT

 

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