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CRIMIC CR00484

Gene CG6543

CG6543

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:13854024..13854031	50C6	FBti0199596	79270

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	OK	CACCATTTGCTTGGGATCCCAAAATGCC	CATTTAGCTCGTTGTTGCGATGACTCCG

Images

Construct Design Sequences

sgRNA sequence	TGCCCAGCTCCAACCTAAGCCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CTCCTTGCGATCGGCCTATT	ATATCAAGTGCCGCCGGTTT	ATTCCGGCTTAGGTTGGAGC	TCTTCAGGAGAACGGGGAGT

 

Amplicon 5'

Amplicon 3'

CTCCTTGCGATCGGCCTATTGGATGAAAGAAAATACAAGAGATTAAATAGTGTTAAGCAGATATTTAAACTTGAACTAACCGTGGAGAACGTGGCATGGAAGGTGCGACGCTCGAACTTCAGGCCCTCTTGGAGAGTGGTCTCGTAAGCCGTGTTAACCGCCTCCTTGCACAGCTGCACGATCAGATTGGAGTGGGTGCCGATCTTCTCGCCCAGCTTGACGGCCTCGCCCAATAACTGGTCGGCGGGCACCACCTTGCTGGCCAGACCCAGCTTCTCAGCCTCCTGGGCGCCGATCATATTGCCGGTGAGGCACATTTCCATGGCCTTGGACTTGCCAACGACGCGGGTCAGACGCTGGGTGCCACCGGCACCGGGAATGGTGCCCAGGGCAATTTCAGGCTGACCAAACTTGGCCTTGTCACCGGCATATATGATGTCGCACATCATGGCCAGCTCACAGCCACCGCCCAAGGCGTAACCATTCACGGCAGCAATGATGGGCTTCTGGGTGCGGGCCACCTCTGTCCAGTCGTTCAAGAAGTTGCCCTGGATGCACTGCGAGTAGGTGTTGCCCACCATCTCCTTAATATCAGCGCCAGCGGCGAAGGCCTTCTCGCTGCCCGTCAAAACGATGGCGGAGATGGTCTTATCCTTGCTGAATTGCTGCAATGCGGTGGACAGCTCCTTCATCAGGCCATTGCAGAGGGCATTCAGGGCCTTGGGGCGGTTCAGGGTGATCACGCCTACGTTCTTGCCCTCGCCGGCCACTTCGGTCTTGATGTACTCCCAGTTGTTGTTGGTGGCTGAAAAGAGATGGCATCATAAATGAGGTGATCGTATGTGCGCGAATGTTACGCAATCAATGTTTATTTGCCTGGAAAAGTACAAAAGATGTCGCCAAGAAACCGGCGGCACTTGATAT

ATTCCGGCTTAGGTTGGAGCTGGGCATCAATCGCGTGGACTTTGATCCCTCGCAATCAACCATCTACTTACAGGAGCTGCTGAAACGGGTGGCCACCTGTGGCTGACGGGCGGCTGCCTGCAGGACGCACTGGGCGCGGCTAGCGAAGATCTTAGCAATGTTGGCCATTTTCAATTGAAGGAACTCTGAGCCGGCTGCAAGTGCAATTCGTTATCTAATCTGTGGCGGTATCTGGCATGGAGCCACTCTAGAACTTGGGATACTTACGCTGCGAAGCACTGGAGCAATATTGGCGCACTAGTGGGGCAGGTGTGACCGCGTTCGCTCCCACCAGAAATGCCACTATTTCCAGTCACTGTTTCAGTCACAGTTTGGGTCGCAGCAACAAGTGGTTCTAGCATTACAGCTGTTAACGCAAAAGTATTGTTAACAGCCGAAATTAAAATGTGCAACCTAATAACACAATTATTATGTTCTTAAATCTGTTTTAATATATTTACAATATAATACTACTTCTCACGTTTTACTCTAAAAATAAATATACATTCGTATTTAAGCTTTTAATTTTTATGGAGAACTTGAAATAACATTATTTACATAAGATAAGCCTAGATAGTTTGAAATTAACAGTACTTTTTATCAACAACTTGGCTGGTAAATAAGTAAGTATGCGTGATCACATGTTTAGCTTTGTAAAAATTAATTCCTAGACCTTTGGTATTGAAGATGTCTGGGGAAGATTACTGCTGGTCTCCACCAGCCAAATGTGCTCCTCTCGTTGCCTTCTTGAGCCATTTTCTCGACGCACCCGCCAAATTAGAAACACCAATACCACGATTAGGAAGCAGAATATTACTATAACCAAGCTGAAAACATACGCTTGGGGTGCTAAAAGTAAATAAATTAGTATTAATGAAGAAACTTTTGATAAATACACAACTTACTTTCACAGTAAGGAAACTTCCTAGCCCACAATGCGTCCTGGCAAATGTTCTGGTTCTCGCCTTTCAGTCTGTGCTCCGCCGGGCAGTCGTAGTAGATACGGGTGCCATTGGCTACCCTTGATCCTACAGTGAGGATTGCATCTGTGAAGTGCTTAACCATAACGTTTGGCAGTTGGGGAACCACGCACTCCCCGTTCTCCTGAAGA

 

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