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CRIMIC CR00486

Gene Zpr1

CG9060

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	X:9161242..9161255	8C14	FBti0200567	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	short band	CTCTAAGCTTGGCTCTTTTTGTACTGCC	ACAGGCAATAGGAAGAGTTTCCGACC

Images

Construct Design Sequences

sgRNA sequence	ATGTGACTCAATGGTTCTTTTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CAAGGAATCGCTGCATCTGC	AGTCACATAATTCGTATTGTTCCCC	TGGCTATCTCCACTGGTTAAGT	GCAGACCGACGAGATAGTGG

 

Amplicon 5'

Amplicon 3'

CAAGGAATCGCTGCATCTGCTGCTTGGTCGCGTCGTCGGCGGAGTCGTGGAAAAGCGTGCCGTCCAACTGATCCCGCATGGCAACCAGCAGACCCTCCACGGTGGTGAATCGCCCGCACAGGGCATGCGGACCCACCTCGAGGTCCAGCTCTGGAATAGACATGCTGCACGTCTCCGACTTGAGGACATCACGCGTCAGATCCTCCCTGCTGGCAATCTGGACGCGGAAGCGTACACCCTGCGCCTCCACTCCGCCGCCGGACTTCACTTCGTTCGTCTTGTGGCCACAGGCACCGCAAACGGTGGCCATGATCACCACCTCCTTGAAGTGCGGTATGTTTGTCAGCTTCATGTTCGTCTCGCAGGGCGCCTGGCAGCTGGGACAATTGGTGGGGAACTGAAGCACTTCGCCGTGCAGATTCTCAATGGGCCACGAGTCCTCGGCAATCGGCTTCAACAAGTGCTGCTCCTCGTGATTCTGCTCGTACAGGCCTGTAAAGGAATACAGGTGTGTGGTTATAAGGGGAACAATACGAATTATGTGACT

TGGCTATCTCCACTGGTTAAGTTTCTAATTTTACAAAGAGGCCTCACCCAATTGCTCGTTCTGCTGCTGGCTGCGTGTGAAGTACGAAGTCTTTAGCTGCGGATCGGCGGCAGGTGCCAGGGGATTCTCGATGAAACTATTGCCGGATATGTCCTCGAGAAGCACTTGAAAGGGTGTTGTCACCTCCTTTAGGCGGTGCAATCGCTCGATGTACTTTTCAATTGAAGCAGCTGTCTCCGGATGATCGATGCGCCGCTTCTCCTGATCCTGCGACAAGCCGGCTATCGTCCGCTCGATGATGCCTTCGACGGTGGTCACCTCGCCCTTCTGCGACTGCACCGGAATCTCTAGCTCGATCTCCGGTATGCTGATGCTGGAGTTATCCGAGCGCACCACGCGCCGATTGAGATCAGCCACGGATTGCACTCGCAGTTCGATGCGAATGCCGGACTTTTGGATCTCCGATGCCGACTGCATCTCGTTGTTGATGTGGCCGCAGTGGTCGCACTTGAAGGACATGAGCACCACCTCGCGAAAAAAGGGTATCTTGGTGGGCAGCAGCCGAGTGACTCCCGTTTCGAAGCAGTTCATGCAGGCGGATTCGACCTCCACTATCTCGTCGGTCTGC

 

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