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CRIMIC CR00487

Gene CG3328

CG3328

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:24168227..24168250	60C1	FBti0199385	78924

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATGGATACCTACCTACGTAGAAGCAGG	AAAGAGCTTGTGCCAGTTCTCCGG

Images

Construct Design Sequences

sgRNA sequence	GTGGCTACTTGAGAGCCAATGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AACTCCAAGCCCTGAAGACG	CACCTTCGTTCCGGGGAAAT	CAAGTGGCCGTACCTACCAC	TCGGGTTGATGATGAGCTCG

 

Amplicon 5'

Amplicon 3'

AACTCCAAGCCCTGAAGACGATGATCCGCTGCTGGGGTCCCGCCGATCCAAGGGCACCCGTTTTCATTCCGAGCAATTAGATTTCGTTTTTATCGCTTGGTTACCATGCCGATCCGTTCGCAGTGGACTCCATCGTTTGATTATTAGTTTTACGGCCCGTTTCGGGCATTATTTTCGCTGCTCAGCCAGTTGTGAAGAGTGCATCAACCGTATGCTCGCTCATACGTGCGTTAGATAATTGTTCCATCGGATGCAGTGGATCGCAGCCGCAGCAGCAGCCGCAACAGCAACAGAAGCATCAAAGTCGAATTGGGCATCCCATGGTGTGCGCATGGATGCTGGCAGTTAGAGCCAGCTAACTTGGCCCACAGTTCGGACTAAATCGAAGAATCCTGCAAACACAACCCCCCACTGGCTATGGACTATCCCAAGAACTTGAGCGGTAACTCATCAACATAATCATGAGCATAGCATAATAATCCGAGTCCCCCCATCCGCGGCACCTGCATAAATTACACAACTCACTTGGGGCACCAGTCAACAATCCGTTTGGCCAATTATCGATCAAACACAGAGCGGGAATTTCAGGGCTTATCAGCGGCAGCTCCCCGCTTCCAGTGGCCCGAATAAAACGAATATTTTGATTCAACTATAATTATCTGCGGGCTGGTAGACGCCCCCTCAGCAACGTGCCACACTTAATTTCCCCATAATTGAAATGTTTTTCTGCCATTCTGATTTGCAGTCCGAGTAGCGGGCGATTCCCGGACTTTGCTCTACTTCGAAAGATAATAAACTGTTGGATGTCAATATTTACTCCTTACCCCCGTTCCTCTGGATACAGAATTTGTATATTTCCCCGGAACGAAGGTG

CAAGTGGCCGTACCTACCACGTAGTCTTGTAAAATTAAACCACCAACTATAACTATTACAACTGAGCTCCATATTCAGAACCATCCAGATTCGCTTGGATTTCAATGTTTCCTTTGCACACAAACTTGAGATGAAGGGAACATTACGATACACTTGAATAAATTTGTACCGCCTTTTGAACTAACCACCATGTCTTACACTGTTGCAGCTCCCCAAGATCTGGATGACATAAACAGCGATGGCATGGACCATCTGGATCTGCTGGTGCAGGGGGCTTTCGACGATGGCATCCAGCTAGGCCACAATGGCCACCTGTCCGCTGGAGGAGGAGTGCACTCACACATGGAGTCGCCGCACACGTCGCCCATGAACGGCTTGGATGCCCACCTGACCAACTCCGGCCGCGTGGGCAGCACGCCCGTGGCGGCACAGGCCCAGAGCCACGTGCATCCGCAGCTGCCGGAGAGTCCGCCGGATTCGCAGCCCGCCTACAGTCCGCTGGGCGAGGCCCACGGGATGGCGATGCCGCTCCAGCGAGAGCTAATCTATCCGGGTCTGGCGCCCATGCAGCACCAGGCCAATATGCTGCAGACGGATCTGGGCCAGTACGCCTCCCCTCCGCAGCAGCAACACCACTTTGCAGCTCCGCAGGCCGATGTGCGGATCAAGCACGAGTCCGAGCTCATCATCAACCCGA

 

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