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CRIMIC CR00489

Gene robo1

CG13521

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:22699146..22699151	59A3	FBti0199386	80923

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGTAAATTTTCGGGTCGGTGCGACTG	GCCAGAACTTCTATCACCATCTCACC

Images

Construct Design Sequences

sgRNA sequence	CGGAGACAAAGCTGGGCGTGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GGGCAGTGTTTGATTGGCAG	GCCCAGCTTTGTCTCCGATT	GGGCAGACAAGTTGGAGAG	ATCGCCACGTATCATCGAGC

 

Amplicon 5'

Amplicon 3'

GGGCAGTGTTTGATTGGCAGGTCCGATTTGAATAATCGGAGGTGGACGCTCGTCTAACGAGCTGACTTGCAGGAAAACCCGTACTGTAGAGGAATCGACTACACTGAAAGCGGAACACACATAGTAGCCTTCGTCTTCCTGCCGCACATCCGTAATCTGCAGAGTTCCATCGGCAGCCACATGCTGCCTCCCGTGCGAACTATTTGGGAACATGAGAGTGGATACTCCTTCCTTGGTCCAGAAAACAGATGGCGGTGGGTTTCCGGAGGCCATGCAAGGTAGTTGGACAACACCATTTAGTCCCACTTTCTTGTTACTGGGTCTTTTCGTAAAGTTCGGCGGAGCTTTAATGAAAACAATTGAAGATTAAAACGCGATTCTTTGGTAATGGAACTTAATCTTACCATGGACTATAAGAGAAGCCCTAGCGCTGATCTGACCGACATTGTTGTGTGCCTCGCAGACATAGGTGCCCTCATCGGTGGGCGTTATGTTGGATATCTCTAAACTTTTCTCGTCGTGAAGGATTCGCGCTCTGGACACCGGAATATTGCCCTCCTCCTTTTTCCACAACACTTTCGGCGGCGGATCACCGCCCACTGAGCAGTGGAAAGTGGCTGTCTGGCCGTAGAGCATCACCTGATCCTTGGGCTCCTTCATAAAGTATGGTTTGACCTGGACAATCAGCTTGGCATAGCTGCTCTCGCGGGTGCCTACCAGATTCTGGGCAATGCACTTGTAGTTGCCCTCATCAATGGGCTCCACATTGCTGATCAGCAGGTTGCCACCGTCCACAATTCGAACGCGGGAGCTGGCGCCAAACGACATGGCTTTCAGGTCGTCCAAGGGAACGCCGTCCTAAGAGCACAAAAAGCGGTTTAGACAACTGACTTTCGCAGTCTGGTTTTCGGAACCCACCTTTATCCAAATCAGCGTTGGCTCTGGAATGCCTTTGGGCGGCCCACACTCCAGCAGAGCCGTCTCGCCTTTGGCCACTCGCGTGTCTTTGGGCTCCACGCGAAAATCGTCGCGCAAAACTAGCGAAGAAAAATTGCAAAAATATCAAAAAAGCACATTATAATCGGAGACAAAGCTGGGC

GGGCAGACAAGTTGGAGAGCAGACTAGAGTGGCGGACTAACTTACCAGCTATCTGGAGCGAGGCATGGCGACTAACGGCCTGGCCCACTCGGTTCTTGGCCACGCACCAGTACTCTCCGCCGTCCTGTTCCTTCTTGCCCTGCATTGTCCTGTAGAAGAAGAGGGCGCCGTCCTTGAACTGGACGCGGTGCGATTTCTTTTCGTTGGTGCTGACGGGTTCGCCATCCTGAGAGCGGAAAGAAGAGGGGGTGATTGAGTTACGATTTGGTTCGGTTTCGAAGGCTACACGGAAAAAAACAACTACTCAAAACCACTTATATTTTGTATAATTTTTGAATTCATTTTTGTTTTGATATTTAAAGATAAATAAATATACAATATTGATATGAAAACATTAAGATCAAGTTTTCTTTTGCCAGTGTAATGGTGATTCAAGTTAGAAAAGTATTATTTGGTGGGTACCTTAAACCACTCAATGGTGGGTTCCGGCTTGCCCTCCACTTTGCAGTTGAGCGTGGCGGGTTCATTCTTCTTAACGACCAGATCCGTGGGATGCTCGATGATACGTGGCGAT

 

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