CR00490

CRIMIC CR00490

Gene Cpsf5

CG3689

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3L:9412059..9412059	67B5	FBti0200568	83177

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	AGATGGGGTTGCTGCGCAATTTCAGA	CTGAAATGCTTCAGCTGGTGGACAAC

Images

Construct Design Sequences

sgRNA sequence	ATATAAAGTACATGGAGAGATGG
sgRNA2 sequence

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GGTAGAAAGTCGGCCAGCTT	TTCTGGATACACGGTGTGCT	GAATATCCATCTCTCCATGTACTT	AAAACACGCGAGATTTGGGC

Amplicon 5'	Amplicon 3'
GGTAGAAAGTCGGCCAGCTTGTCCCGCAAATTTTCCCACAGGGGTGTGTGTATAACAAAATGGCGTCCTCGCAAGTCTCAAACAAATCGGGCTCCGGTTGGCCGCGCCGCGGAAGCCAAGGCCAAGCGGATGCAGCCAGCAGCAATAACAACGGTACCCAGAAGTACACCAACCAGGCGCTAACCATCAATCGCACCATCAACTTGTGGGTACAACACATTTCATTCATAGACGCACATCATAGATCGATCGATTGTTTCAGATACCCCCTTACGAACTACACATTCGGCACCAAGGAGCCGCTCTTTGAGAAGGATCCGTCGGTGCCATCCCGATTCCAGCGGATGCGCGAGGAATTCGACCGGATCGGAATGCGGCGCTCCGTCGAGGGAGTGCTTCTGGTCCACGAGCACGGACTGCCACATGTCTTGCTCCTGCAGCTGGGCACCACGTTCTTCAAATTGCCCGGTGGCGAGCTAAACGCCGGCGAGGATGAGGTCGAGGGCCTCAAGAGATTGCTATCCGAGGTAAGTCAAACGTGGCGCCATAGTTAAATATTGTTATTTGATCTTAACTATCACTACTTTTATTATTTCGTAGACGCTGGGTCGTCAGGACGGCGTAAAGCAGGAGTGGATTGTTGAGGACACGATTGGGAACTGGTGGCGGCCGAACTTTGAGCCACCGCAATATCCATACATTCCGCCGCACATCACCAAGCCCAAGGAGCACAAGCGGCTTTTCCTCGTCCAGCTACATGAAAAGGGTAAATATCCGCAGGGCGTTTTGTAAGTCCCTACGCGTAGGTATAACGAGCACACCGTGTATCCAGAA	GAATATCCATCTCTCCATGTACTTTATATAACCTATAGATTGTTTTTGTTTGCCAAAAACCGAATACGCTAACAAACCTCATGTCATATCTTTTCAATGCTGTTTAGATACGGACGGGAACGCCAGTTACGATGTACCCTGAGCAGTTCCATTAGCGCCGTGTGTGTCGTATCTACCCAACTCTAATCTCAAGAGTGCAATGCCATAGAACAACAAATCGAGCAAACAGAACAGATCAAACAGAAAAGAAACAAACAGATCGCAAAAGCTGGCGCAGGCGACTCCTTAGTTAGTTTTGATTCTAGTCCAATTGTACAACATGTCGAAAATCAAATTCAAATTGTATTTTTGCTCAGGACTGAGTGTTGGCATATTTTGTTATTTCCCGATTTTTATCATTCGAAATTCAAAGATTGTTCTTGTACTTAATTTTGTAAATATGTCGACATACTTGTGAGACTGAGCGCAGCAATGTTATCCTTCTATTTACATATTTTATTTATTTCCTAGCTTCTATTCTTACTTTTGTGAAGTGTCATGTGTCGTTCATCTTAAGGTTGTTGTGTATTTATCGAAAGCAATCCAATCCATCGGGCAGGCAGCAGAAGAGCATTGTAACGTTGCTGGCTGTCGCAGTCATGTGCCCAAATCTCGCGTGTTTT

Amplicon 5'

Amplicon 3'

GGTAGAAAGTCGGCCAGCTTGTCCCGCAAATTTTCCCACAGGGGTGTGTGTATAACAAAATGGCGTCCTCGCAAGTCTCAAACAAATCGGGCTCCGGTTGGCCGCGCCGCGGAAGCCAAGGCCAAGCGGATGCAGCCAGCAGCAATAACAACGGTACCCAGAAGTACACCAACCAGGCGCTAACCATCAATCGCACCATCAACTTGTGGGTACAACACATTTCATTCATAGACGCACATCATAGATCGATCGATTGTTTCAGATACCCCCTTACGAACTACACATTCGGCACCAAGGAGCCGCTCTTTGAGAAGGATCCGTCGGTGCCATCCCGATTCCAGCGGATGCGCGAGGAATTCGACCGGATCGGAATGCGGCGCTCCGTCGAGGGAGTGCTTCTGGTCCACGAGCACGGACTGCCACATGTCTTGCTCCTGCAGCTGGGCACCACGTTCTTCAAATTGCCCGGTGGCGAGCTAAACGCCGGCGAGGATGAGGTCGAGGGCCTCAAGAGATTGCTATCCGAGGTAAGTCAAACGTGGCGCCATAGTTAAATATTGTTATTTGATCTTAACTATCACTACTTTTATTATTTCGTAGACGCTGGGTCGTCAGGACGGCGTAAAGCAGGAGTGGATTGTTGAGGACACGATTGGGAACTGGTGGCGGCCGAACTTTGAGCCACCGCAATATCCATACATTCCGCCGCACATCACCAAGCCCAAGGAGCACAAGCGGCTTTTCCTCGTCCAGCTACATGAAAAGGGTAAATATCCGCAGGGCGTTTTGTAAGTCCCTACGCGTAGGTATAACGAGCACACCGTGTATCCAGAA

GAATATCCATCTCTCCATGTACTTTATATAACCTATAGATTGTTTTTGTTTGCCAAAAACCGAATACGCTAACAAACCTCATGTCATATCTTTTCAATGCTGTTTAGATACGGACGGGAACGCCAGTTACGATGTACCCTGAGCAGTTCCATTAGCGCCGTGTGTGTCGTATCTACCCAACTCTAATCTCAAGAGTGCAATGCCATAGAACAACAAATCGAGCAAACAGAACAGATCAAACAGAAAAGAAACAAACAGATCGCAAAAGCTGGCGCAGGCGACTCCTTAGTTAGTTTTGATTCTAGTCCAATTGTACAACATGTCGAAAATCAAATTCAAATTGTATTTTTGCTCAGGACTGAGTGTTGGCATATTTTGTTATTTCCCGATTTTTATCATTCGAAATTCAAAGATTGTTCTTGTACTTAATTTTGTAAATATGTCGACATACTTGTGAGACTGAGCGCAGCAATGTTATCCTTCTATTTACATATTTTATTTATTTCCTAGCTTCTATTCTTACTTTTGTGAAGTGTCATGTGTCGTTCATCTTAAGGTTGTTGTGTATTTATCGAAAGCAATCCAATCCATCGGGCAGGCAGCAGAAGAGCATTGTAACGTTGCTGGCTGTCGCAGTCATGTGCCCAAATCTCGCGTGTTTT

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