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CRIMIC CR00491

Gene Topors

CG15104

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:18975795..18975798	56A2	FBti0199387	78925

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGCGCCCGTTTTTACAGGTCTGTTCGTA	TCCTCTTCCTCTTCTTCCTCCGCA

Images

Construct Design Sequences

sgRNA sequence	TAACACCACACATTCTGAACAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCTCACAAACGGTTGCCATT	CAGAATGTGTGGTGTTATACATACA	CAGGAAAACACTAGAATGATCTCT	CCAAAGTTTGTGGGCTGTCG

 

Amplicon 5'

Amplicon 3'

GCTCACAAACGGTTGCCATTAACCTGAGCATGAGGCGCCCAGCGGCGGTTGCCAGCGAGGAACCAGAGGTCATCGAGATCGACGACGGCGATGCGGCCGCAAATGCAGAGGTGGCGGCCATCAATGATGGCAGCAACACCAGTAGGAGACACGCAGGTGCTACTCTTCCAGTGACGGCGCACATCGAACTGGAGAGCAGCAGCAGTTCCGGCGATGAGGATGAGTGCGTCTTTGTGCTGGAGCTGAAGCCACCACACATGCGCACTCCCGAACAGGTGAGCCTTGACTCAAATAGTGACTCCGATGTGGTGTTCGTAAATGAGCAGCACGAAGCTGCGCCGGACGCAATTGCCGAAAACCGGTCGACACAATCCCCTTTGGATCTGGCCTCCAGGGATCAGGGCCTGTTTATGGGGCCCAGCACAAGTGGCGCAGCGGCCAACAGAGGCAAGAACTGGAAGCTGGTCATGGCGCAGACACGGCGCCTCGATCAGTTGCGCACGCTGCGCTCTATACGTTCGAAGAAATCACGCCGTTCATCCATGCCTGCCCGCAGTGACAGTGGCAGCAGCCCCAGCAGCTGCAGCAGCTCCTCCTTTCACTTCAGCAGCAGCAGCGATGAGGATAGCAGTGACAGTAGTACCACCAACTCCGAGCCACCCAAGAAAAAGTCACGCAAGCGAGTGGCGAATAATAAGCGCTCTAAAAAGGAGTCAATCGGGAAAAGGACATCTCGTAAACGGAAGGCCAAAGACCAGAATATGGAAATGCTTGAGCAGCAGCAGATAAGCCAAAAGAAGCCCCAGCGCCAACCGGAAAGCAGCAGTGACAGTCCAAGTTCGTCCGATGACGAGTCAGGTGGCGATAGCAGCGAGTCATCCGGTAAGTGGTTTGTGAATCTAGACGGTTCTTATCAGCATGTATGAATGTATGTATAACACCACACATTCTG

CAGGAAAACACTAGAATGATCTCTAAAAACAATCAAAAAACTATTTATTATCCTATCAGATCAAACTTGTATAATTACACAAATTTGTTTTCTAGGTCAACCCAACACGAACAACAACAAATCCAGCAGCGATTCCGATGATGATAGCGCTGTAAACATGCAGCTCAGTGCCTTGCGCGCCACACTAAAAGCGGAGGCAACTCTGGAGGATAGGAAACCTGTAAAACTGGAGCTTCAGTTGCCGGATGACGACCAGGCGGGGCCCCTCCACTCACGGATGACTCCATCGCCGAGAGAGGATAACGAACCGGGTTGCAGTGCACCGAAACGGCGACGCAGTTGCAGCCACAGCAATCAGTCCAGCCAGAGTGCCAGTTTGGCCAGCAGTTCGACAGCCACCTCGAGCTCGGCTCCATTAAGTTCGTTTGCCTGGGGAGCAGCGGGCTTTTCCGGCGATCCGCTGATGCGCGGACATCCGGCGATGGAAGAGCACGACATAGCCAACTCCCTAATCGAACTGTCCACTCTAACGCAGCCTGTGAACATCGGACTCTTCAACGAGCATTATAATTCGGCAGAGAATTCCATGGGCATGCTGAGCAACACGTTGTGCGACAGCCCACAAACTTTGG

 

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