CR00493

CRIMIC CR00493

Gene Cbl

CG7037

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3L:8426110..8426110	66C12	FBti0199598	79272

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	CGGCATTTTACATGGGTCAACGATGAC	GATTTTGCACAGCTGAAAGGTGCTGC

Images

Construct Design Sequences

sgRNA sequence	GCGCATAAACATTTGTACCCTGG
sgRNA2 sequence

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ACGCCAGGTGCAAGGATAAA	CTGGGGTAGCTGCGCTAAG	CCCAGGGTACAAATGTTTATGC	AGAATCTCTCCCTCGGCAGT

Amplicon 5'	Amplicon 3'
ACGCCAGGTGCAAGGATAAAGCCGTGAGGATCGGGCACCCAATCGGATAGATCGCGTTTGGTTAGCTTGTGGGGGAAAATCGTACTTAAGTCACCACTACTACTACACACGGGCACCACCAGCAACACCAACAACAACAACAACGAGAACAGCACCAGCAACAACAACAACAGCAGCAAGAAGGAGAAGAGCTGAGAAGAGGAAGCAGAGGCAGCGCAGTCGGCAGCGCAGCGGCAGAGAGAAAAGATGGCGACGAGAGGCAGTGGAACCCGTGTGCAATCGCAGCCAAAGATTTTCCCATCGCTGCTTTCCAAGCTGCACGGCGCTATCTCGGAAGCCTGCGTCTCGCAGCGTCTGTCCACCGACAAGAAGACGCTGGAGAAGACCTGGAAGTTGATGGACAAGGTGGTCAAACTGTGCCAGCAGCCGAAGATGAATCTTAAGAATAGTCCACCGTTTATTTTGGACATCCTGCCGGATACGTACCAGCGCCTGAGATTGATCTACTCAAAGAACGAGGACCAGATGCACCTGCTCCATGCCAACGAGCACTTCAACGTGTTCATCAACAACCTGATGCGAAAGTGCAAGCAGGCCATCAAGTTGTTCAAGGAGGGCAAGGAGAAGATGTTCGACGAGAACTCCCACTACCGCCGCAATCTCACCAAGCTCAGCCTGGTCTTCTCCCACATGCTCAGCGAACTGAAGGCCATATTCCCCAACGGTGTCTTTGCCGGGGATCAATTTCGGATCACCAAAGCGGATGCGGCTGACTTTTGGAAGAGCAACTTCGGTAACAGGTGAGTGGCGCCTATTGGAAACCTATTGGACAGTGAAATATCGTGACCATAACAATGCCAAGTCTAATTGGTATTATTGGTAACTAAATGAATTTAAAGTGCGGTTTCAAAACCAAGTGTTATAATTCAATTCATTCATTGTGATTTGATATGGATAATATTACTAGAATTATAACTCTGAATATCCAATTTCGTGTCTGAATCAAACCAGTTTCAACCGGTTCGGTCTTAGCGCAGCTACCCCAG	CCCAGGGTACAAATGTTTATGCGCATATTTTCAATTTAAAAGGACAATTCTACAAAATGGATATCACATATGTTGTTAACAAAGACCACACATTATATATTTTGAGTTTTCTTCTCGGGTTTCCAAGCTGTTCAAACATATATGATTGTGAACTCCCTTCCCAAGTAATTTATAAGAAGGTCTTTTTGTGTTACATTCGAATTCCCCTAAATAAACTTTATAACTCCGCATTCTAACAAAGAATATATTTGTGCCAAAGCAAAATAATAGCACATACTGACATTTTTGCCTTCCTATTGCAGCACATTGGTTCCCTGGAAAATCTTCCGGCAGGAGCTTAACAAAGTACATCCCATAATCTCCGGCCTGGAGGCCATGGCCCTAAAGACCACTATCGATCTTACCTGCAACGACTTCATTTCCAACTTCGAGTTCGACGTCTTCACACGCCTCTTCCAGCCTTGGGTGACCCTGCTACGCAACTGGCAGATTCTGGCCGTCACACATCCGGGCTACGTGGCGTTTCTCACATACGACGAGGTGAAGGCTCGCCTACAGCGCTACATCCTCAAGGCGGGCAGCTACGTTTTCCGGCTCTCCTGCACGCGATTGGGCCAATGGGCCATCGGCTACGTAACTGCCGAGGGAGAGATTCT

Amplicon 5'

Amplicon 3'

ACGCCAGGTGCAAGGATAAAGCCGTGAGGATCGGGCACCCAATCGGATAGATCGCGTTTGGTTAGCTTGTGGGGGAAAATCGTACTTAAGTCACCACTACTACTACACACGGGCACCACCAGCAACACCAACAACAACAACAACGAGAACAGCACCAGCAACAACAACAACAGCAGCAAGAAGGAGAAGAGCTGAGAAGAGGAAGCAGAGGCAGCGCAGTCGGCAGCGCAGCGGCAGAGAGAAAAGATGGCGACGAGAGGCAGTGGAACCCGTGTGCAATCGCAGCCAAAGATTTTCCCATCGCTGCTTTCCAAGCTGCACGGCGCTATCTCGGAAGCCTGCGTCTCGCAGCGTCTGTCCACCGACAAGAAGACGCTGGAGAAGACCTGGAAGTTGATGGACAAGGTGGTCAAACTGTGCCAGCAGCCGAAGATGAATCTTAAGAATAGTCCACCGTTTATTTTGGACATCCTGCCGGATACGTACCAGCGCCTGAGATTGATCTACTCAAAGAACGAGGACCAGATGCACCTGCTCCATGCCAACGAGCACTTCAACGTGTTCATCAACAACCTGATGCGAAAGTGCAAGCAGGCCATCAAGTTGTTCAAGGAGGGCAAGGAGAAGATGTTCGACGAGAACTCCCACTACCGCCGCAATCTCACCAAGCTCAGCCTGGTCTTCTCCCACATGCTCAGCGAACTGAAGGCCATATTCCCCAACGGTGTCTTTGCCGGGGATCAATTTCGGATCACCAAAGCGGATGCGGCTGACTTTTGGAAGAGCAACTTCGGTAACAGGTGAGTGGCGCCTATTGGAAACCTATTGGACAGTGAAATATCGTGACCATAACAATGCCAAGTCTAATTGGTATTATTGGTAACTAAATGAATTTAAAGTGCGGTTTCAAAACCAAGTGTTATAATTCAATTCATTCATTGTGATTTGATATGGATAATATTACTAGAATTATAACTCTGAATATCCAATTTCGTGTCTGAATCAAACCAGTTTCAACCGGTTCGGTCTTAGCGCAGCTACCCCAG

CCCAGGGTACAAATGTTTATGCGCATATTTTCAATTTAAAAGGACAATTCTACAAAATGGATATCACATATGTTGTTAACAAAGACCACACATTATATATTTTGAGTTTTCTTCTCGGGTTTCCAAGCTGTTCAAACATATATGATTGTGAACTCCCTTCCCAAGTAATTTATAAGAAGGTCTTTTTGTGTTACATTCGAATTCCCCTAAATAAACTTTATAACTCCGCATTCTAACAAAGAATATATTTGTGCCAAAGCAAAATAATAGCACATACTGACATTTTTGCCTTCCTATTGCAGCACATTGGTTCCCTGGAAAATCTTCCGGCAGGAGCTTAACAAAGTACATCCCATAATCTCCGGCCTGGAGGCCATGGCCCTAAAGACCACTATCGATCTTACCTGCAACGACTTCATTTCCAACTTCGAGTTCGACGTCTTCACACGCCTCTTCCAGCCTTGGGTGACCCTGCTACGCAACTGGCAGATTCTGGCCGTCACACATCCGGGCTACGTGGCGTTTCTCACATACGACGAGGTGAAGGCTCGCCTACAGCGCTACATCCTCAAGGCGGGCAGCTACGTTTTCCGGCTCTCCTGCACGCGATTGGGCCAATGGGCCATCGGCTACGTAACTGCCGAGGGAGAGATTCT

Search Again