• About
• Search
• MiMIC
  • Technique
  • Constructs
  • RMCE Lines
• Transposons
  • GDP
  • Others
• CRIMIC
  • Technique
  • Constructs
• Order
• Downloads

CRIMIC CR00497

Gene Uba5

CG1749

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	X:11483974..11483975	10B15	FBti0199390	78928

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACACCATACACCGTACACCGCAAACA	TTCCTCTGACGTTTCGCTGGACTTCT

Images

Construct Design Sequences

sgRNA sequence	CATATTTGGAGTGCTAGCTGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCCATCACTAACATGCAGCG	CACAATGACTGTTGCCCT	CACCACAGCTAGCACTCCAA	TCAGCGTCTTCTCGTCGATG

 

Amplicon 5'

Amplicon 3'

GCCATCACTAACATGCAGCGTTGCCCCATCCCATCTAACGCAAGTACCAAACTAATTTGGCCATCTGGCAGCACTGGTCAAAAGTGACACGGCGACAACCAGCTTAAAAGCGAAAGTTAACAATTAACAATTGCTGGCGTCCATAGCCAAACACACGAGCCAGAAACTGGAGCCACCCCAAAGCCAGAATGTCGCACGCCATCGACGAACTGCAGGCGATCATCGCCGATCTGAAGACGGAGCTGGAGACGGAGCCGAAATCCAGTGGCGGCGTCGCTAGCAACAGCCGCCTGGCACGCGATCGCATCGATCGCATGTCCGCCGAGGTGGTGGACTCCAATCCGTACAGCCGCCTGATGGCCCTGCAGCGGATGAACATCGTCAAGGACTACGAGCGCATTCGCTACAAGGCGGTGGCCATTGTGGGCGTGGGCGGCGTGGGAAGCGTCACCGCCGACATGCTTACACGGTGCGTGCACAACAAGAAGAGAAGGGCAACAGTCATTGTG

CACCACAGCTAGCACTCCAAATATGTTGTCTTTAATTAAAGGCTATGTATTAATCAATTGATTTCCTGCAGCTGCGGCATTGGCAAACTGATACTCTTCGACTACGACAAAGTGGAGCTGGCCAACATGAACAGACTGTTCTTTACGCCCGATCAGGCGGGCCTCTCCAAAGTTGCGGCCGCCGCCGCTACCCTGAGCTTTATCAATCCGGACGTGGAGATTGAGACGCACAACTACAATATTACAACGGTGGAGAACTTTGATCGCTTCCTGGACACAATATCGCAGGGCGGACGGATAGCTGGCCAGCCGGTGGATCTGGTGCTCAGCTGTGTGGACAATTTTGAGGCACGAATGGCAATCAATGCAGCGTGCAATGAACGCAATCTCAACTGGTTTGAGTCGGGAGTTTCGGAAAATGCCGTCTCGGGGCACATTCAGTTTATCCGTCCTGGCGATACCGCCTGTTTCGCTTGTGCACCACCCTTGGTGGTGGCCGAGAACATCGACGAGAAGACGCTGA

 

---

Search Again