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CRIMIC CR00500

Gene bur

CG9242

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:21177130..21177134	39B1	FBti0199392	78930

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGCCGATTACTTCGGGAATTCGAAAAG	GATCTGCGAGTTCGATGAGATACGCA

Images

Construct Design Sequences

sgRNA sequence	GCAGCATTGTATCAGCGCTTAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CGTGTCATTGTGGTGCGTTT	CGCTGATACAATGCTGCCAG	AGGTAAACGCATTTAAGCCT	GCGATGCACAGCGAATCATT

 

Amplicon 5'

Amplicon 3'

CGTGTCATTGTGGTGCGTTTTGATTGTTTCTGCATTCGTGCTCACCATGCTAGAGGCGGACTCGATCAGATCTGGTCGGAGGGTTCCCTGGGCCAACATAACTTCTTCGGGCTTTAGTTTCAATTCGGCTACTACATCATTGGTCACCTTGACGAATATATCACCAATTATCTTGCGTTTTTCCTCCGGATTGTATGTCTGACATAACATCGGCGTTTCCACCACGGAGTACTGTCCGGGCCTCTTGACCTGCGTGGTGCCTTTAAGGAACGTGTAGCCTTCTTTTCGGACGATTAAATCAATGCCAATATCGCGCAGTGAACGCTCCACCTTTTCACTTTCCTTCTTGCGCATGAAACCTTGAAGTAAGGGATAAAATATTAGGTTTTAAAGTACAAATGATATAAATTCGGTATTCACCGAGTTAATGAATTATTTGACGTAGAAAAAATATAAATGAATAAATTTCGCTAATCTGTCTACTGTCATACCATTATCTACATGCACGGCAATTATCTGATGAGGGTACAAAGCACGGCGGAGCAAAGCTGCACAGACACTCGAATCCACGCCGCCGCTGACCAGGAGCTGTAAAATGTAGATTGTATTGAGATATTCATGTCTTGATTGAAAATTTATTTTCCCAAACTCACCAACACCTTATTGTTGCCCACTTTCTCACGGATATAGCGTATGCACTCCTCCTTTCGACTACCCATGGTAAAGTTAGGTGTCAGTTCGCAGATTTCGTACAGGAAGTTCGATAGCATCTGTTTGCCATTGATAGTGAGGTCCACCTCAGGATGGAACTGTACGCCGTAGATGCGTAGTACTTCATTGTAAATGGCTGTCACAATGCGGTTTGTAGACCAGCCACCAATCTTCAGATTCTCGCCTACCCTCTCAACGCTATCTCCGTGGGTTAACAGCACGGACTGTGTGCGACTGAGGCGACTGCGGGAAACGAAGATGTTATGTTTGTTAATTGCATGTATTAGAATAATTAATGGTAATGATAGATAATATGTTAGGCCACGTGTAGATCAGTTTATAAACTACTCTAAACTAAACTGTTCTTGGCTTTAAAATTGTTCTGGCAGCATTGTATCAGCG

AGGTAAACGCATTTAAGCCTAAGCACTTTCTTGTTGCTGCTTGTTTTGGGAATCAATATTTCCTGGCTCACCTAAAGAGCGGGCACGAGGTCTCGATCTCGATATTTTGTTGGCCATCCTCTCGAACATCCTTCTTGAGCACTGTGCCCCCGAACTCTTTGTTGATTAGCTGCATGCCGTAGCAGATGCCCAGCATAGGTATTTTTAGCTTGAACAGATCGGGATCATAGCTGGGCGCATCCTCAGCGTAGACTGAGTTGGGTCCGCCGGAGATGATGATGCCTCGATAGCCATTGTTGCGTATCGTGGCAGCTGGCGTATCCAGAGGAAGGATATCCGTCTCAACGAAGAGTTCGCGTACCTTACGGTCGATAACCTGCGGAGAAACCAGGACCATTTTAAGAAGAGGTTAAAACTACTCTTGGTTTTAAGCTTACCTTGCCGTACTGTGCTCCCGCATCAAGTATAACAATCTTATCATGCCGCAGGCCATTCTCTGCTGTGCCCAGAAAAATGTTTGAGTTCATTTCTTTATCGTTGCAGCACTGAAAAGAGCAAAGATTTGAGTGTCAGTCAAGCAATCGCAGAAACAAACACCGAATCTCGACTGGCTGGTAAACGCGCTTCTGCTGCTTATTTATACTCCAATTGTGGCTGCAATCGACGCTACAGAAACAAGGCTAAAAGCTGGCTAGGAGGTGGGGAAAATAGGTTTCGGATTGGGGCAATCGCGGAGATTGAGATTTGTGGTACAGAAGGCTCACATACCGGCAATATCTTGTGGAGCACTCGCAGAAACATTCAACATGCAGCTGGCCAGGACGCTCTACGATAGACAATAAACATTGGCAAATATTTTCTTGTCACAGAAAGATGATTTGTGCAATGTGGAAAGAAATTTACAAGCGACGAAAAACAAGGCCGAGAGACGCCTACCAAAAATGATTCGCTGTGCATCGC

 

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