CR00502

CRIMIC CR00502

Gene chp

CG1744

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3R:31208254..31208279	100B6	FBti0199393	78931

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATGGTGAACCAGTCAAAGGTGAGTCG	AGCTGATTGAAGGCCATGTCCAGGAT

Images

Construct Design Sequences

sgRNA sequence	AGGAACTTGAGAAGCCTTAGAGG
sgRNA2 sequence

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GAAGGCGTGCGATTGAATCC	TCCTAAGTTCGTGTGTGCGA	GGCAATCCGATTAGTTCCCC	ACCATCTCACCGAGATCCCA

Amplicon 5'	Amplicon 3'
GAAGGCGTGCGATTGAATCCCGGAGAGACTGGCCCTGGTGATCTGCAGATCCTCCAGCTCCACGCCGAAGTCCTTGAAGTCCTCGGGTCCCACGGAGGAGGATCCCAGGCGCGAGATCGACAGTGATCTCATGTTCCGCATTCTGGTCATACTTTTTGTAAATAAATACAAGAGTGGTTCTGGGGTGAAGGGTGTGTGGGGAGCAGGATGTTTCTCGGTGCACTGGGTTCACTTACTTGCGCAGGGTCTGCAGATTGGTGGGGTCGTTGCGGTCTCCACTCAGGTCCAATTTCAGGAGGGTGTAGTGCACCGCATTGAAGGTCTCCGTGGGCTTCTGGATCTTGATCTTATTGTCCCGCATGCTGATGACCCTATTATTAAGAAATAAATGTATAGAATTATAATTTACCATTATAAAATTCAATAATAGCTATGCAAATCAAGCTTTTTCTCAGTGCCGCGTATTTTTGCAAGATTGCCTGATTGCGCGGCTTTTCCCTCTTCCTCGAATTCTTTTAAATAGGGTCACCCACTTGTCGCACACACGAACTTAGGA	GGCAATCCGATTAGTTCCCCCCAATATGCACATGTGCAAATGGAATGGGGGCAGAACGTGAGTGAATCGGAGTGCAGGTGCTCGACTCACCTCAAGACATCGAAATTGTTGAAGAGCTTATGATGCAGCTTGGTGAGATTGTTGCCAGACAAATCCAGGATCTGGAGGCTGTTCACCAGGCTGTCAAAGGCCACAGGCGAAATGTTGGTCAGGCCGCAGTAGCGCATGTAGATCTCCCTGATTCGAGCGGGCTTAAATGCATCTTCCTGAAAGCGAACCGTAAGTTCCAAATATGTTATTTATAAATCAAATTTCTGGGAGGGATTTTCGTCCAGTTAAGTTTCACAAGGGTTAGTTAACCTACCCTCAGAGTGTGAATGGGATTGCCATCGAAGAAGGTGCGGTTGACGGTATCGAAGTACTTGAATGAATTGGGAGGCAGGACCTCGATGTGATTGTACTCCAGGTGTAGATTGTCCAGATTCAGCTTGGTGCTGGCCTTGATCTCGTAGGTTTCGTTGCCACTGAGCGACCAGATCACATTGTGGGAGATATCTAGGGTGCGAAGGCTCTTTAGCGGACCCAGGGCGTCGTACGGAATCTCCGAGAGGATATTGTCCGTGAGCAACAGACGGGTCAGCCTCGGCATTCCATCGACGAACACATTCGGATCGATCTCGAACAGGTTGTTCCCACTCAGGTCGAGGGTCTCAAGAATGAGGAGCCCGGAGAAGCTGTGCGACATCAGCTGCGATATGCAGTTCTCGCGCAGGATCAGCGTCTGCAGCGAGTCCTCCAGGCCGCGGAACGAGTCGTGCTGAATGTGCGTTATGTGGTTGTAGCCCAGGTCCAGATGACGCAGCTTCTGCAGGTGCCTCAGGGACTTGGACGGGATCTCCACCAGGTCGTTCTGCGGCAGAATCAGCTCCCACAGCGATCGCTCCAGGCCGGTGAAGGCATCATCTGGGATCTCGGTGAGATGGT

Amplicon 5'

Amplicon 3'

GAAGGCGTGCGATTGAATCCCGGAGAGACTGGCCCTGGTGATCTGCAGATCCTCCAGCTCCACGCCGAAGTCCTTGAAGTCCTCGGGTCCCACGGAGGAGGATCCCAGGCGCGAGATCGACAGTGATCTCATGTTCCGCATTCTGGTCATACTTTTTGTAAATAAATACAAGAGTGGTTCTGGGGTGAAGGGTGTGTGGGGAGCAGGATGTTTCTCGGTGCACTGGGTTCACTTACTTGCGCAGGGTCTGCAGATTGGTGGGGTCGTTGCGGTCTCCACTCAGGTCCAATTTCAGGAGGGTGTAGTGCACCGCATTGAAGGTCTCCGTGGGCTTCTGGATCTTGATCTTATTGTCCCGCATGCTGATGACCCTATTATTAAGAAATAAATGTATAGAATTATAATTTACCATTATAAAATTCAATAATAGCTATGCAAATCAAGCTTTTTCTCAGTGCCGCGTATTTTTGCAAGATTGCCTGATTGCGCGGCTTTTCCCTCTTCCTCGAATTCTTTTAAATAGGGTCACCCACTTGTCGCACACACGAACTTAGGA

GGCAATCCGATTAGTTCCCCCCAATATGCACATGTGCAAATGGAATGGGGGCAGAACGTGAGTGAATCGGAGTGCAGGTGCTCGACTCACCTCAAGACATCGAAATTGTTGAAGAGCTTATGATGCAGCTTGGTGAGATTGTTGCCAGACAAATCCAGGATCTGGAGGCTGTTCACCAGGCTGTCAAAGGCCACAGGCGAAATGTTGGTCAGGCCGCAGTAGCGCATGTAGATCTCCCTGATTCGAGCGGGCTTAAATGCATCTTCCTGAAAGCGAACCGTAAGTTCCAAATATGTTATTTATAAATCAAATTTCTGGGAGGGATTTTCGTCCAGTTAAGTTTCACAAGGGTTAGTTAACCTACCCTCAGAGTGTGAATGGGATTGCCATCGAAGAAGGTGCGGTTGACGGTATCGAAGTACTTGAATGAATTGGGAGGCAGGACCTCGATGTGATTGTACTCCAGGTGTAGATTGTCCAGATTCAGCTTGGTGCTGGCCTTGATCTCGTAGGTTTCGTTGCCACTGAGCGACCAGATCACATTGTGGGAGATATCTAGGGTGCGAAGGCTCTTTAGCGGACCCAGGGCGTCGTACGGAATCTCCGAGAGGATATTGTCCGTGAGCAACAGACGGGTCAGCCTCGGCATTCCATCGACGAACACATTCGGATCGATCTCGAACAGGTTGTTCCCACTCAGGTCGAGGGTCTCAAGAATGAGGAGCCCGGAGAAGCTGTGCGACATCAGCTGCGATATGCAGTTCTCGCGCAGGATCAGCGTCTGCAGCGAGTCCTCCAGGCCGCGGAACGAGTCGTGCTGAATGTGCGTTATGTGGTTGTAGCCCAGGTCCAGATGACGCAGCTTCTGCAGGTGCCTCAGGGACTTGGACGGGATCTCCACCAGGTCGTTCTGCGGCAGAATCAGCTCCCACAGCGATCGCTCCAGGCCGGTGAAGGCATCATCTGGGATCTCGGTGAGATGGT

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