• About
• Search
• MiMIC
  • Technique
  • Constructs
  • RMCE Lines
• Transposons
  • GDP
  • Others
• CRIMIC
  • Technique
  • Constructs
• Order
• Downloads

CRIMIC CR00503

Gene Eaat2

CG3159

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:740260..740274	21E2	FBti0199394	78932

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAACGGAGCCGTTAACAAACAATCGC	ACGGTTATTTCATCTGTCGTTGGGCC

Images

Construct Design Sequences

sgRNA sequence	AGCGTTAAGCTCACATGTAGAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ACAGAACTAATGCCCACGGG	GCTTAACGCTGCAACTAGTGT	GCGCCACTGAGGATGCTAA	AGGTGGCTCTCCGAGAATCT

 

Amplicon 5'

Amplicon 3'

ACAGAACTAATGCCCACGGGTGTCAGCCACATCACGCAGGTGACCATCTTCATGATCACCTCAAAAATGGCGGCAAAGAAGTCAACCACGACCTGACCCTTCTGGCCGATGGTTCCCAAAAAAGTACCAAACACCAGGCAGAAGAAGACGATGCCCAGGGTGTTGGTTCCACTGCGGTACTGGATGTCCCTCACCAGCACCACATCCTCCGTAAGGTCTTCAGACAAGCGTTGGGCCTCCGATCCGGATGCCAAGGTGTCATTCATCGTTTCGTTGAAGACGTGCAGGATACTCGGCTTGGGCAAGTAGACGGTGTGCGCCTGCTGAATGGAGGCCTGAAACAGGTTGTCCGGGAATACGTTCCTGAAGAAAAGAAGAAAATAAGAAAACACAGCTAATATTGAAATATGAAAATCAACTCTTGGAGCATATAAAAGATAAATATGTGTATGATTGTATCACAATTGGGTCGACTTTTAATGTCGATCTACTACAAACTACAAAATAATTTTCGTTGCACTGCTTCAAGCAGTTATATTCGTAAACTGCTTAGTCGTACTACAGAATCAGACTGCTTGCCAGGTTAAGACACTAGTTGCAGCGTTAAGC

GCGCCACTGAGGATGCTAACAGGTTTTATAGCTTTTTATGTTGCTAAGAGCTACTTTTCGTATGCTACTTAAATGTAACTCACCTGCCCAAATCCAAAAGACTATCTAGAAGGTTAACTGCCCGCCGATCTGTAGATCGATCGTCCGCATTGTGCAGATCCGGATTTCCAGGATGGATGAGCAGAACGAGAGCGATTCCCAGTGCAGCGTTAAAGAACGAAGTGCTGGCAAAGTAAACCAACGTGCGCAGCGCTATCTTGCCGTTCATTTTGGCGTTAAGGCTGGCCGATCCGGCAATAAGGCTCGATATAACTAGCGGCAAAATCATCAGCTTGAGTACGCGCATAAACAGTTCGCCAGGATAGGAAATCAGCATGATGGAGTCCCCATGTAAATTAAGCGGCCGTAAGGAGAGTCCTGTCGGAAAAGTAGTACATTAATGGAATATTTCAGCATAACTATATTGCGTTGGCAATCAAAGTGTATGCACTAAAATTAGGTTGTCTGTTGAACTAATCGTACTTACCTAGTATTACACCAAGGAGAACTCCGGAAAGGGTGACCAGGAGCATTAGATTCTCGGAGAGCCACCT

 

---

Search Again