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CRIMIC CR00504

Gene CG8080

CG8080

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2R:9091196..9091210	45A2	FBti0199395	78933

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AAATGATCATCGGGATCGGATAGCGG	GCGATCTCCTCTGCATTTTGTCGTAG

Images

Construct Design Sequences

sgRNA sequence	CGAGGCACGCAACACAACATTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CCATGTCCTGGTCCAGCATT	GCCTCGTCACTTCGTCACAT	TTGGTGGCAGTGATTGGAGG	TTAATCGCATCTGACGCCGA

 

Amplicon 5'

Amplicon 3'

CCATGTCCTGGTCCAGCATTTCGGGTGCGGTGCTGACCTGCTCCATCTTGACCTCCGTGTGGCGGAAAAGGTCAGTCGGCTCCGGAATATTGCCGTTGCTACCAAGCATCGTAGTCCGCACCCTCGAGCGATGCATCCACTTGAAATCTCCCTGCCAGGAATTTGAATATTTAATGAATGAATTGTGAGAAGTGGCTCGTTCCAGGACTTACGCTCTTGATGCGGGAAACGGCGTCGGCCGGATTATCCGAGTAGTGCTTGGGCAGCATGAGTCGGCCCTCCGAGTGAAGCGGATCCGAGTTGAAGCCCACGATCGGCGTCTTCTGCTGGCTGAGCGCGAAGAGCGGACTGGCGCGTCCGGCGGAGAGAAGGAAAGTGCCATCGCCGCCCACCGGCACGATGACGTCCGCCCAGCTCATGACGTCCTTGCTCAACGAGCTTCTAAATTCAAGTCTGCTCGTTTAGTAAAGTCAACACACAGGGTGAGGTGCCGCATGGGAGACACGAAGTGTGTGAAGATGGTGGAGATCAATTGTAGCGGGTAGTAGTCACAAATCGAAGATACATATAGGTATAGTATGTGACGAAGTGACGAGGC

TTGGTGGCAGTGATTGGAGGCCTAAAGGTGTGGATGTCTGGGTACATGTCCTCATAGAACTAATCTCATTCAAAGCAATGAAATCATTTCCATGGCTTGAGTACATTTACTCATTTACTTGAGTACATGTACCTATGGCCTCCGTTGCTTTGAGCTGCACTCCGATCACTGAATTGGAGGACTACATGGGTACGCACCTGCTGGACAACTTCACCTCGCAGCCGACGTCCTGGAAGCTCTGCACGACCCGCCGCTCGAAGTCCTTGTGCACCTTGTGCAGATGGAGGACCATCTCCACGTCGGTGCCCCGGTCACGGAGCTTTTGCTGGAGCTGATCCGGAGACAGTTCCGGGTGGCGTAGCTGCTCGAACTCATAGCGGGACAGCTTGGTGACGACCAGGGCCCGCTTTAGCTTGAAGTTGGTCGGCGTATATTTAGCGTACTCTGTTCAAAATGGCAATTGCAAGCGAAAGACCTCGTTAGTCCGATCAAATGTGGGTCCTCGTCACGGGAAATTTACTCAAACCCTCGGGAATAATCTTCCTGCCGTTTTGAAGCATCCTACGGCCGAAATCGATGTGTAACTACTAGGTTTTAATGGAAATTTCCCGGAACAGCTGATAAACAACACAGTGCAAGTGGAAAGTATTTTCCCTCTAAAACTTTTAATGAAACAATGTTATGTATTTGGTTTGTACCAATCATGTTTTTCGTAGATTTTCCTAATGGTTTGACATACTGCAGTGAGCTTTAAATACCATTATTAAAGATAAGTAAAATCAAGCTATCCAGTATGTAAAATTTGTTTGTTTATAATCAATATAATTATAGCATAACAAAGTTTAGTGACACACACTTTGAGATCTGCGTTCAATACTTGTTGAAAAGTTGGTTAAGTTAAGTTAATCCGGAAACCAAAAAGGATATTTAAGATAATATCCACTTTCTGAGCGAATAATTTTGTCAGCCAGTGTGCGTGGGGAAAGTTCATCGTGACAAAGAATTTGGCGAAAAAGTGTTTACTGATAATCTTCGGCTTGGTCGGCGTCAGATGCGATTAA

 

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