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CRIMIC CR00505

Gene Rbf

CG7413

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	X:799207..799213	1C5	FBti0199396	78934

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTCTGTGCATTGCCTGCCTAAAAGGG	TGCGATATGCCCGCATAATGTCACTG

Images

Construct Design Sequences

sgRNA sequence	GGCTTATCTCTATGTCCAAATGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TCCAGACACGTCTCCTCGAT	GTCGTTGGCGTCGTGTAAAC	GCCATTTGGACATAGAGATAAGCC	TCAGCCTGACCCCGTGTATA

 

Amplicon 5'

Amplicon 3'

TCCAGACACGTCTCCTCGATGGAGTTAAGGTGCTTTATCAGCGACCGGTTCAAACAGCCTTCGTGCGAGCCGTGCCGCACCACGATCTCGATGATCTTTTGGAACTCGAACGCGCTAATACTGAAACAGTCGAGCACCCACGGGAACTTGAGCTCCGTTTTGTAGGCCTCCAGCACCAGCTCCACGCAGCAGGCCATCAGCGTGATGTTAAAGATGACCAGGGAGACCTTCTGCACCAGCAGTCGCTTGAGATCGATGTCGGGCTTGTTCCGAATTTCCGCCTGCAGGATCTGGTCGAGCAGGTAGAAGAAGAAGGACTTCGCTAGTTGGAAGCGACTCTTCGCCTCCGTTGACGGGTACTTGGCGAGGAATTTCTGCTCGATCTCCTCGATGATGCTAAGCAGCTTGGCTATTACCTCTTCGCCAGCTTGCCTGGATAAAGACAGGGCGATATCAAAACATGAGCTATAGAAATCAATCCCGGATTTAATCTAATCGATTCTTCTTACATCAACAGAAATTTCTATGGATGTGTGCTTCGGCTAACGAAATTCGATATTCGATATTTCCTACGATTTATAAAGCAATCAAATAAACATGAGTTTTGTTTACACGACGCCAACGAC

GCCATTTGGACATAGAGATAAGCCTTTTCTCTCTCACAGGTTTTGAATGATAGTTAGGAACACTCGACACCCAACCCAAATACAAGGCCAGCCGGCAACTCACTTGACAAAGTCCGTGGGTTCTGTGATCCGTCCGAAGGCGCTCAGCTGCTTCACGTTGTTAGTGGCGTTGCGGACGGGCTCAAATTTATCGCCAGCCATCGCGGGCTGCGCAGGCAGATCTTGCTTGCGGGTAAGCGGAGTAACCGGCGGACGCAGGGACTGGTCGTTGAGGGCAGTGTGTTCCCCCGCATCGTAGGCACTCAGAATGCGTTCGTCGAATTCGCCGACGCTGAGGACGTACTGCTCGTAGCTGATGTTGAGGGACTTTAGGTTGCGCTCGAAGTTTTCGTTAGCCAATAGGCCTAACATAGTGTCCTTGTTGCCGTAGATGGTCTGGTCGAAATGAAAATAGAACAAATTAATTGTTTTAATGGAAATCCGAAACGGCACTCCTACCGAAGCCTGAAAGAAGCTCGACATGATCTGGCGGAAAGTGGTGGCCTTCATGGCTTTAGCCTCCTCGGTCATGTCACAGAAGTTGGACAAAATGCAGTGCGGATTGTGACGAAAGTCCAGCTCCGTCCAGTTGCTCGGCAGGCCCTCGAATTTGGGGTTGATGAGATCGGTGCGCTTCTCGGCCAGCACATTGTTGTATATCAGGTCTATGCAGCAAATCATCAGATTGTAGGATGTCACCAGGTCCACGGTGTTGCTCGGCTTCTGGTTCTTGGCGCAGAGAAACAGGCGCCAGCAGATGTCGTCCAGCTTGATGTACGAGCACTTTCCGTGGGCGTGGTTGCCATGCAGAGAACTGTTCCAAAGAGGGAAGCTTTTTATATACACGGGGTCAGGCTGA

 

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