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CRIMIC CR00506

Gene betaCOP

CG6223

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	X:18383360..18383383	17A9	FBti0199397	78935

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTGCAGCATCCCAATGAGTTTCTGCG	TGACCTTGTTCTCCCACTCGAAGTC

Images

Construct Design Sequences

sgRNA sequence	TAATGGGCATGTCTAGCTAGAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TGGATTGAAAGCGATGCCCT	TGCCCATTATCTATTGTTCGGC	TCAGTGCAAGCTGATGC	CCGCTGGCACATTGATTACG

 

Amplicon 5'

Amplicon 3'

TGGATTGAAAGCGATGCCCTTTCGCCTAAAGATGCACTACTCACTGGTCTGGTTGACGATCAGCACGTCCAGGACAATATCGTACTGATTGACATTGACGTAGGCCTCCGCATAGACGGGATCCGAGAAGCCAGTCAGTTGGGTGACCTTGTTGAGCTTGCTGTTCGGCGAGGCCACGTCGCTCAGCTGGGCGTTCTTGCTGCCGGCCAACGCCTGGTTCAGACTGGATTCGAACACATTCTCGCCCAGCTGATTGTCACGTCCGTTCGAGAGCTGTGCAAAGAGCACCGGATCGTCCGGCTGCACCTTGGCGGTGGCCTTCTGCTTCTCCTTCAACATGCGCTGGTCCTCGTCGTGCTGCGCATCCAGCATCTTGCCCAGAGCCTCGCGGCAGTACAGTGTGAAGACGGAGATAGCCTCCGGCGTGCGTTCGCTCAGCGTGCGCAGGCAGACGAAGATGCGATCGGTGTCATCGTTGGTTATGGGCTTGCTAGGGAAACCGGACTTGCCCAGGTGCAGTATCGAGCTCATGATCAGCATTACCTGCGTGGTCAGACGATTCTGGGCACGCGCCTCCGTCTCCAGCTCTGCGTAGCGCAGTGCCAGCTTGGTCAACGTGGCGGATAGAGCGGCGCCGATGAAGAAGTCGCCGTCCATCAAGTACTGACGCAATGGCGGACGTTTCTCCGCCTTGGCCACCCTGCAGAAAGCATTAACCAGCGTTAGAATCAGAACTGCAAACAACTATTTTGTAGGTGCTACGTATTAAGATGACTCATTGCCGAACAATAGATAATGGGCA

TCAGTGCAAGCTGATGCAATGCCACAAGCCTCGAATGCCTCATTGGTGGCATGATATACGTACACGTAGCCATTACTTACGGGGCCAGACTGTAGGCACTCTGGGTGGCATAGGTTCCATCGGAGGTGACCTTGTTGCTGGCGTTTCCGGAGCCAGAGCCCTCTGCGGCGGATCCAGCTGCATTGCCGCCTGCGGATCCCTGCTGCTGCTTCTGCTCCTCGGTCTGGTCGCCGGCCAAGCGACGCTGCTCCGCCTCCACCATGGGCACTTCGCCCAGCGTCTGCTGAATGACGGCAATCACCTCCAGGATTTGCGAGCCCTCCACGTATTCACCCAAAATCCAAACGGCGGCGCGATGAATTTTGGAGGACTTAATCTGCGGGAAGGCCTCAATTAAATGCTCAATGATGAGGGCGCGCAGGGCGGGGAACTTTTGGATGGCTTCGCGTATGAAGATAAGCACATCGGCGGCGGCAAGTTCGTTGGTATCCGAGAGGAATTCAACCAGTACGGGTATAACATTGGCGGCCACATCGGGAAACTTGATGGAGCAGGTGTGCAGGGTGCGGACGAGCAGCTGCCTGTACTTGCCAGTGTCCTCGTGCTCCACATTGTGCGTCTTGGCCACCTCCTTCTTGAGGACCAGCACCATTTCGCCAATATTACGCGAATAGACCAAGTCCAGGGCCAGCGCGAGTGTTTTCCTGCGCACCTCGATGTCTGGTGCGGCCAGGACGCGCAGAACGTCCATCACCAGATCCTGCATCACCTTCTCCATGCCCTCGTGCTCCTTCATGGCCACCAGGCGATCGAGCACGATTAGCTTGACGTTGTTGTCGCTCTCCTTGACCACCAGTTCGATGTAGCAACTGGCCGCCGCCTTGATGGCCGTGGGCGCCAGCGAGAGCGTAATCAATGTGCCAGCGG

 

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