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CRIMIC CR00508

Gene Brms1

CG4400

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	X:12908861..12908867	11D1	FBti0200571	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	short band	TTGGCGATTCGCTCGAGCACCATATA	CCAAAAGCTAAGCCAGGTGAGCAGAT

Images

Construct Design Sequences

sgRNA sequence	CCATATCGTCGCAATTAGATTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CTGCGCCACGAGAATCAATG	TGGCTTTCGTGTTGATAAGGC	ATTGCGACGATATGGTGGCC	CAGCTAGAGATGGCAGACGG

 

Amplicon 5'

Amplicon 3'

CTGCGCCACGAGAATCAATGAGCTGAGCGGTCAGATACACTTCCATGGTGACCACGTGGACGTCCTGCGTGACTATCTCAAGGAGAAGGGCTTCTGAAGGTCCACAAATGAACCGTCTGTGCCGCAATAAAAGCTTTTCTGTTGATTTAAATCATGTGGGATTTGTGTTCTTCTGTCTCGCCTCACCTAAGGCGGCTTACCCACATTGTGACCTCCTACCCATAGTATCCAAGTCTGGATAACAAAACTGCTGGCCTTCCGGAGATTGAATCACTCGCAGGCAATCGAATTAAATTTATGTTGAACGCATGCCCACTTGGCTGCAGTTCGCTTTTATTTGCACTTATCTTTTAGCATAAATTACATACATCGTCTGTATGGGGGAGGGGAGAGACACACACACACACACACACACACAATATTCTGAGAGTGGGACTCGTACAAACTACGTACGTACTAAAATCTACGGGAAAAGGAGGTGAGGATGGGTGGCGGAGCTTAGCCATTTTGATGAACGCATTTGAAGTCTGGTTCGGGGGCTCTGCTACGGCTACTACTGATACATAAACGTCTACGGCTGCTGCTTCCTCGGGCGCGGCCGTCAGGTACGCGTCATGTGGGCGTAGGCGGCAGCTGCCACGCCCCAACGCTATCCGCTAGCTCCTGCCATTGCTGGCAAACCGGACAGGCTAACGCTTACGCCGGAAGTGGGCGTAACTGTGCCAGCAGCGGTGGCCGACGAGGAGGATCGCTTGAGGGCCTTGCGTATAATCGTCCAGTCCTCCATGATATCCTCCTCGCGCAGCATGTACACGACATAGGGACCCGTGACGGTGACGGCCTTCTTGCGACCCGGTCCGCGCACCTTGCTCTGCCGCTTGTCGGTGCCCCAGTCGGCCCACGAGATGTCCACGTTGTGGCGATCCTCCTCCAGGCGACGGATGCGCTCCATGAACTCCTCGCGCAGATTGTCCAATGCCATGTGTTTCTCGCTCTGCAACGGAAAAGTTGTGAGTTGCAAGAGGATACATTAACAACTACTTCTACGCTTAAGTTAAGCCATCATTTGTCTTAAATCGTTAATATATGGCCTATAAGTGAGTGACATTTTGTGTTGTAATTAGCTTTGGCAGTGTCGCCCCCAGTGCCTTATCAACACGAAAGCCA

ATTGCGACGATATGGTGGCCAATTAGCTCTTCTCATTTTTGGCGCCAAAAATCGAGATTGGTTATGTACCTCAAAGTGCTGGACGGCAGCTTGTTCCTCGGACTGGTATTTGTGCTCGATATTCTGCAGTCGGTACTTGCGCAGGACGTCCGCCACCTCGATCCTGGTGCGATACTTCTTGTCCAGTTCCTTTTGCGGCTGGACAAACTCCTCGGAACGCCCGGAACGCACCTCGGCCAATTGTCGTTCGATGAGATTGATGCGCTCGACGTAGTACTGTTCCCGCAACTCGTTAAATTGGCGCTCCAAGCTCACTGTGTCAAGGAAAGAAGAAACTCGATGAGTATGGCTTGTGTTAAGTGTGTACGTACTGATTCCTACGCAAATCCTCGATGTGCTCTGCCCGCCGGCGGTCTATTTCACTGGCATCCAACTCGGAGGAGTCATCCGAATCGCATTCGTTGGCCTCCTCCTCGTCGGAGGTGTCGTGTGGCTGGCTGGAATTCGAGTGCTCCGATTCTCCGCCAGAAACGTCGCCTTCGCCATCGGATTCGCCATTCTTCACGGGCATTTCAGAGTGGATTTTGCGGTTCTCCTTGTTCTCGGCGTATTCCGATTCGGCAATGGACTGCAGGTGGCCGCCGGTATAAGCGCGATATCTTTTTTTGTTTGCTTTCGACAATCGGGGAGTGTGGAAACTGTGGAAAACTCTGTGGAAAAACACTAAACTGTGTGCTGTATCAAAAAAAGTATGACCGCACGTAAGAGTGTTGGGCAATTTAGTGTTGGTCAACGCAAGCGGAGCATCTAGCTGAGTGGCTACAAAATAGCTAAATCTGCGAAAAATATTATTCATTAAGTTAAACACATTAAAATATTAATGTATCATTGAATTTGAAGTAACCTGGACCACTAAAATAAGAGAAAATAAATTTGGAATTTACCCATGTAACTATTTAAATATAAAAATGTTACAATATTTTTGTTTGATGAAAAATAATGATATTAACTCTGTCGTAAATTAAATTATAAATAGAAGGTCATGGAAGTAATTTCCGATATTTAGATTAAAGGAATACATCTTTAAAATATGGTTCATTCCCATATAATATTGTGGACTTATTTGAAATAGCCAAATCTAGGCATTTTCGGCTGCTCAGCCCCACCGCCGTCTGCCATCTCTAGCTG

 

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