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CRIMIC CR00511

Gene ATPsyndelta

CG2968

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	X:10213886..10213886	9B4	FBti0199600	79627

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	short band	TAACGCCAGTTTTGAATGTTGCGCGG	ATCGCTGCTCCGGCTCCAGTTTAT

Images

Construct Design Sequences

sgRNA sequence	TAAATGCCAGTTGGCGTGAATGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AACAGCGGCTGATCACTGAA	ATGGTGGAAGCAGTGTTTCC	CTTCCACCATTCACGCCAAC	CATTCCCACACAACGCCATG

 

Amplicon 5'

Amplicon 3'

AACAGCGGCTGATCACTGAAGTTTTCTCGTGTTTTTCGCTATCAAACCGAAATAAAAACAGGTGAAAATAACTGATAAATTGAGGGGTAGTCCACTGATCACAGTGCCCGAGGATTTGCAATGAAAAGCGATCGAAATTTGAGCTGGGGAAACGCAATAGCAGCCGCCGCCTAACCTACAAAGTTACATAATTTGGTTATTTTGTTGTTATGCTGGCTGGCTGTGGCAGAAAAGACTTTTAATCCTGAATTTATCCTCTCGTTGCAGCCCAAAATGTCCTTCGTTAAGAACGCCCGTTTGCTGGCCGCCCGCGGCGCTCGCTTGGCCCAGAACCGCAGCTACTCGGATGAGATGAAGCTGACCTTCGCCGCCGCCAACAAAACCTTCTACGATGCCGCTGTGGTGCGCCAAATCGATGTGCCTTCCTTCTCGGGATCCTTCGGCATCCTGGCCAAGCACGTGCCCACTCTGGGTAAGTTCGATCATTCAGTTTCAGTTGCCTCGAGATGGTTATATCACCGTTGGCGAATCTCTTTGCTATTGCAGCTGTCCTGAAGCCCGGCGTTGTCCAGGTGGTGGAAAACGATGGCAAGACCCTCAAGTTCTTCGTCTCCAGCGGTTCCGTCACCGTCAACGAGGATTCCTCCGTTCAGGTTCTGGCCGAGGAGGCCCACAACATCGAGGACATCGATGCCAATGAGGCGCGCCAGCTGCTCGCGAAATACCAGTCACAGCTTAGCTCCGCTGGCGACGACAAGGTGCGAATCTAGTCAAGAAGTTGCATAGTAGAGTAGTGTCTAGAAAGTAGAAGTAGTGGTCCAGAAAGTACAACTCAAACAGACTCTTTTGATGGAAACACTGCTTCCACCAT

CTTCCACCATTCACGCCAACTGGCATTTAAGTTACTAAACGATTTCTATGTGCCCATCCTTTTTACTTGATATACTTTCTTTGTACAAACAAGTTTGCTCTTAAATTAACGCTTACACATTCGATATTTCCATGAGTAAACTTATGTTTGAGTTGCTTTTGCATTCTGTATTGCACATTCTGAAGCTATCTAAACGAGACTTAACCACGATCCTTGTTTTGCAGGCCAAGGCCCAGGCTGCCATTGCCGTGGAGGTCGCCGAAGCGTTAGTCAAGGCTGCCGAATAGACGTAATCACCACACAACCGCCACCAATAAACCACAATCGATGCTTTGTGTCTGAAATAAATAAAAAACATAACGATCACCTTAAAAAGCCAGAGAGTTATGAAACAATAAAAAAGCGAATCATAATATCGTATTAATGACCCGTGATCCACCAGGCAAATGATTTTTTTTTCTTTTTTTTTTCATGTTATGCCGCTAAACTGTGTGTTTATATAGTTTATAATATATGTATGTGAGCTGGTGAAGAGAAATGTGTAATATTTGCTCGCTGTGGGATAGACCGTTGATATGATAATGCTTTGGCAATCTTTAAGTCAAAAATATGAGTGATTTGCATTTTGGCCGAAAGAGACGAACGTCGTTTCTTTTTCCCTTGCCAATTTATGCAAATCGCATAAGCATAAAAACTTACAATTTGTAAATGAGCTCGTCTTTCATCTTAACAAATAGTATAGTTTGTATTTCATCTGGTGTGTTGCATTCATGGATAGTTCGCATATCTTATCTCGGATGATTGTTTAAATTTCTCTCAAAAGTAGTAATAAAAAGCAACTTTGTTTTTTTTTTATGGAAACAAGAATTAATCAATGGCTAAAATTATACTTTTGGCAGATAGAAGATAATCCATTTTGAAACTCTTTATAAAGATGATAAATACTATAGAATTGAAAAAAGAAAATTGGATTTTTTTTTTTTGGAATTGTCTATGTATTTGGTAACAAAAAAATGTGTTGTAAAAAAAATGTCTTAAATTAACTCATGGCGTTGTGTGGGAATG

 

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