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CRIMIC CR00516

Gene dpr14

CG10946

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	X:7907204..7907218	7C2	FBti0200575	80586

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	no/short band	GCCTAAGGGTTCACAGGTTCATTACC	TTCCCGCGACACAATCTAGGATCTAC

Images

Construct Design Sequences

sgRNA sequence	AAAAGTGCCAAAGTTTGAGTTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CTACCTGATTCCTCCGCGAC	ACTTTGGCACTTTTAAGCTGAAGA	AAGTTTGCAATGTGATTAAGGC	TGAGCTCAATCGCCCGATTT

 

Amplicon 5'

Amplicon 3'

CTACCTGATTCCTCCGCGACTAGTATCGTAGTTGAGCAGGCGCGGTCCGTGCCGCCAGGTGATATAGGAGGAGGGATGCGGAATCTTCGAAATGACGCACTGCAGCTCGATGGTGCTGCCAGCCTTGTAGTACTTCTCCGGCGTGGCCGAGCCCCGCTCGTCGAGTATCTCCACGTGAGGAACTGCGGATGGACAACGAATGAGATCAGCATGGATCAGCAGGTTTTGCTTGGCAAGCCGACAGCAGTTGCTCACCAATTATAGTAAGGTAAACGAGCAGGACGAGCGGCGGATGCGAGGACACCTGGCACTCGTAGGGACCCTCGTCCCGCTCGTTGGCGAACTGGATGAGCAGCTTCCAATCGTTGGGCTCCTCGAACTCCAGCGAATACCGCGAGTCGCCGCTATATGTGTGCTGGCCAAAGGTTATCAGGGTGAGATCATCGCCACGGCGACGCATCCAGGACACCGTCTTGCCCTGCAGATCGTTCACCCGGCAGTGCAGATAAACGCTGGACGAGAGCTGGGTGCTGATGTTCAGCGTGGTGTACGGATCGGCGAAGAACGGGAACGGCTCGTGGGTGGTCGTCTCGGTGACCTCCAGCTCCTCATCCTCGGGCGTGTCGGTGAACGGCGATGAGAACTCCTGCCAGAAGTTCTTCGGAAACGTGTCGAATGGATCGCCAGCTCGCGGTCGTTTCACTAGTTCGAAAAAAAACAATTTCAATAGATATAGTTAAAATATATATGAAAATCATATAATATATAAAAATAAAATGCACTCAGCTAAAATCACGTATAAAAGAAAAACTCAAATAAAACAATAATTTCGTTTCGAATAATGCTTGCACACTAAGTAGTAAAATAATTGTTTTACATAAAAAAAAATAATCATTTTTCACTTGGGTAATCCGCAAAGTAAACAAAAGAACTTAATCTTCAGCTTAAAAGTGCCAAAGT

AAGTTTGCAATGTGATTAAGGCATGAGGTGGAGACTCACATGTGGTCGACGTGGAACCCGAGCCGATGTGATGGAGTGAGGAGTATATAATCGCCAGTATCCAAAACAGCCTCGATCTCATCACTGCAGCGGAGTGTCTTCATATGCCGACTTACGGGATACGGTAACAGGATGCGGGATGATGCATCGACTGGCGCCACTCGCCGCAGGATATGCACATATGAACGAACGCCCCTCTAAACTGTTCTATATCGATCCAAGTCCAACTGCATCAGCGTCCCACTGCATCGCCAGCGATGATTCCACGGACTTCACTTCGCTTCGCTTATCGATTGCCCTGCGGCAAAATATTGCTGGACATATTGCACTCAAGTATCTTCTTTTTCAGAGTGTCCTTTTCGATTATATATGTATATTTACTTTTTCTTTTTCTCTTTTTTGTTGATTTCTTTGCACACTTTTCAGCGCGCGGATGGAGGCTGGAGGGATAGGAATGTCGGACAATTGGAAAATCGGAAAAGCGCTTATTAACCAGCTCGTGTTGCGTGTGTATTTATCCTTTTGCAACTGCTGCTCGGATGGCCAATAAACGGGAATGTAGGTCAGGGCATGCTCTGCGCATGGCCAGCATCCGCATCCCTATTATTTCATCCGAAGGAATCCCTCTACCCGAGCAACAACAACAAATAGCAGACGCCACTCACCTCTCAAGCTCAAAATAATGCAATCTTCAAGATTGTTTTAAAAAGAAAAAGACTTTTCTTTTCTTCTTTTGTTTGCATTTGTCCAGGAAAAAAGCTCTTTTCGGCGGATGGTTCTGATAAATATTTAACAATATAAAAGTTGCTTTTGCCGGTCCTTCGGCGTTGTTGTTCCCTCACCCACACTTGCTTCGGCACTGAATCCGCTCCGAAAGGCCAGCATCTCTCTCGGCATTCTTGCAATCCAGGGATTTCTACCCACCAAACAAGGTCCTTTGAATCCGTATAGCTCGGCATGCAAATAAATCGGGCGATTGAGCTCA

 

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